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Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells

  • Guo, Zhixin (College of Life Science, Inner Mongolia University) ;
  • Wang, Yanfeng (College of Life Science, Inner Mongolia University) ;
  • Feng, Xue (College of Life Science, Inner Mongolia University) ;
  • Bao, Chaogetu (College of Life Science, Inner Mongolia University) ;
  • He, Qiburi (College of Life Science, Inner Mongolia University) ;
  • Bao, Lili (College of Basic Medical Science, Inner Mongolia Medical University) ;
  • Hao, Huifang (College of Life Science, Inner Mongolia University) ;
  • Wang, Zhigang (College of Life Science, Inner Mongolia University)
  • Received : 2015.08.08
  • Accepted : 2016.01.05
  • Published : 2016.11.01

Abstract

Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene and the product of which was thought to be associated with elongation of carbon (C) chain in fatty acids. In the present study, we examined the effects of rapamycin, a specific inhibitor of mTORC1, on ELOVL1 expression and docosahexaenoic acid (DHA, C22:6 n-3) synthesis in bovine mammary epithelial cells (BMECs). We found that rapamycin decreased the relative abundance of ELOVL1 mRNA, ELOVL1 expression and the level of DHA in a time-dependent manner. These data indicate that ELOVL1 expression and DHA synthesis are regulated by mTORC1 in BMECs.

Keywords

Bovine Mammary Epithelial Cells [BMECs];Mammalian Target of Rapamycin Complex 1 [mTORC1];Elongation of Very-long-chain Fatty Acids 1 [ELOVL1];DHA

Acknowledgement

Supported by : Natural Sciences Foundation of China, Natural Science Foundation of Inner Mongolia Autonomous Region of china, innovation team from Inner Mongolia

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