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Evaluation of measurement uncertainty for quantitative determination of chlorite and chlorate in fresh-cut vegetables using ion chromatography

  • Jung, Sungjin ;
  • Kim, Dasom ;
  • Lee, Gunyoung ;
  • Yun, Sang Soon ;
  • Lim, Ho Soo ;
  • Jung, Young Rim ;
  • Kim, Hekap
  • Received : 2017.07.13
  • Accepted : 2017.09.08
  • Published : 2017.12.31

Abstract

This study aimed to evaluate the measurement uncertainty for the quantitative determination of chlorite and chlorate in ready-to-eat fresh-cut vegetables using ion chromatography with a hydroxide-selective column. One gram of the homogenized sample in deionized water was sonicated and centrifuged at 8,500 rpm. The supernatant was purified by passing it through a Sep-Pak tC18 cartridge, followed by chromatographic determination using a Dionex IonPac AS27 column. The linearity of the calibration curves, recovery, repeatability, and reproducibility of the method were satisfactory. The method detection limit was estimated to be approximately 0.5 mg/kg. Each uncertainty component was evaluated separately, and the combined and expanded uncertainty values were calculated at the 95% confidence level. The measured concentrations for 3 mg/kg of chlorite and chlorate standard materials were $3.18{\pm}0.32$ and $3.10{\pm}0.42mg/kg$, respectively. These results confirmed the reliability of the developed method for measuring the two chlorine-based oxyanions in fresh-cut vegetables.

Keywords

chlorite;chlorate;fresh-cut vegetable;ion chromatography;measurement uncertainty

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Acknowledgement

Supported by : Ministry of Food and Drug Safety