Study on microbiological safety of simple processed agricultural products

유통 단순가공 농산물의 위생지표세균 및 식중독균 오염실태 조사연구

  • Sung, Gyunghye (Busan Metropolitan City Institute of Health & Environment) ;
  • Hwang, Inyeong (Busan Metropolitan City Institute of Health & Environment) ;
  • Park, So Hyun (Busan Metropolitan City Institute of Health & Environment) ;
  • Park, Sunhee (Busan Metropolitan City Institute of Health & Environment) ;
  • Kim, Byung Jun (Busan Metropolitan City Institute of Health & Environment) ;
  • Lee, Ju Hyun (Busan Metropolitan City Institute of Health & Environment) ;
  • Min, Sang Kee (Busan Metropolitan City Institute of Health & Environment)
  • 성경혜 (부산광역시 보건환경연구원) ;
  • 황인영 (부산광역시 보건환경연구원) ;
  • 박소현 (부산광역시 보건환경연구원) ;
  • 박선희 (부산광역시 보건환경연구원) ;
  • 김병준 (부산광역시 보건환경연구원) ;
  • 이주현 (부산광역시 보건환경연구원) ;
  • 민상기 (부산광역시 보건환경연구원)
  • Received : 2017.07.13
  • Accepted : 2017.09.08
  • Published : 2017.12.31


The purpose of this study was to explore methods for efficient management of the quality and safety of simple processed agricultural products in Busan. We tested 258 samples of simple processed agricultural products for distribution of aerobic bacteria and coliforms, and identified food-borne pathogens. The average aerobic bacterial and coliforms counts were 7.1 and 4.1 log CFU/g in simple processed vegetables, 6.8 and 3.5 log CFU/g in dried vegetables, and 6.2 and 2.9 log CFU/g in simple processed fruits. Additionally Staphylococcus aureus, Salmonella spp., Campylobacter jejuni/coli and Listeria monocytogenes were not detected in any samples. However, Bacillus cereus, Clostridium perfringens and E. coli were detected in 41 samples (16.3%), 2 samples (0.8%), and 4 samples (1.6%), respectively. This analysis revealed that none of C. perfringens and E. coli isolates harbored pathogenic toxic genes. However, all of B. cereus isolates carried at least 1 toxin gene.


simple processed agricultural product;microbiological safety;aerobic bacteria;coliforms;Bacillus cereus


  1. Kim SW, Park SJ, Byun SY. A study on efficient management for quality and safety of simple processed agricultual products. Korea Rural Economic Institute, Naju, Korea (2014)
  2. Lee SJ, Lee SM. The study on the quality of pre-processed vegetables in school and institutional food-service. Korean J. Food Sci. Technol. 38: 628-634 (2006)
  3. MFDS. Korean Food Standards Codex. Ministry of Food and Drug Safety. Cheongju, Korea (2016)
  4. Flank C, Werber D, Cramer JP, Askar M, Faver M, Heiden M, Bernard H, Fruth A, Prager R, Spode A, Wald M, Zoufaly A, Jordan S, Kemper MJ, Follin P, Muller L, King LA, Rosner B, Buchholz U, Stark K, Krause G. Epidemic profile of shiga-toxinproducing Escherichia coli O104:H4 outbreak in Germany. New Engl. J. Med. 365: 1771-1780 (2011)
  5. Lomonaco S, Verghese B, Cerner-Smith P, Tarr C, Gladney L, Joseph L, Katz L, Turnsek M, Knabel S. Novel epidemic clones of Listeria monocytogenes, United States. Emerg. Infect. Dis. 19: 147-150 (2013)
  6. Kim JS, Bang OK, Ghang HC. Examination of microbiological contamination of ready-to-eat vegetable salad. J. Food Hyg. Saf. 19: 60-65 (2004)
  7. KCDC. Guideline for water & Foodborne Diseases Prevension and Control. Korea Centers for Disease Control and Prevention. Cheongju, Korea (2015)
  8. Kim TS, Kim MJ, Kang YM, Oh GN, Choi SY, Oh MS, Yang YS, Seo JM, Ryu MG, Kim ES, Ha DR, Cho BS. Molecular characterization and toxin profile of Bacillus cereus strain isolated from ready-to-eat foods. Korean J. Food Sci. Technol. 46: 334-340 (2014)
  9. Yang IC, Shih DY, Huang TP, Wabg JYm Pan TM. Establishment of a novel multiplex PCR assay and detection of toxigenic strains of the species in the Bacillus cereus group. J. Food Protect. 68: 2123-2130 (2005)
  10. Sun SH, Kim SJ, Kim GC, Kim HR, Yoon KS. Changes in Quality characteristics of Fresh-cut Produce during Refrigerated Storage. Korean J. Food Sci. Technol. 43: 495-503 (2011)
  11. Bae YM, Hong YJ, Kang DH, Heu SG, Lee SY. Microbial and pathogenic contamination of ready-to-eat fresh vegetables in Korea. Korean J. Food Sci. Technol. 43: 161-168 (2011)
  12. Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim KS, Ha SD. Microbioal contamination levels of fresh vegetables distributed in markets. J. Food Hyg. Saf. 20: 43-47 (2005)
  13. Choi YD, Lee CW, Kim JS, Chung DH, Shim WB. Investigation of hazards from onions and their cultivation areas to establish a good agricultural practices (GAP) model. Korean J. Food Sci. Technol. 45: 785-790 (2013)
  14. Kim BY, Weon HY, Park IC, Lee SY, Kim WG, Song JK. Microbial diversity and community analysis in lettuce or cucumber. Korean J. Soil Sci. Fert. 44:1169-1175 (2011)
  15. Shim WB, Kim KY, Yoon YH, Kim JE, Shin SI, Kim YS, Chung DH. Microbiological hazard analysis for strawberry farms at the harvest stage to establish good agricultural practices (GAP) model based on principle of HACCP. Korean J. Food Sci. Technol. 45: 104-110 (2013)
  16. Park WJ, Ryu HY, Lim GY, Lee YD, Park JH. Microbial Prevalence and Quality of Organic Farm Produce from Various Productio Sites. Korean J. Food Sci. Technol. 46: 262-267 (2011)
  17. Seo Y, Jang JH, Moon KD. Microbial evaluation of minimally processed vegetables and sprouts produced in seoul, Korea. Food Sci. Biotechnol. 19: 1283-1288 (2010)
  18. Jo MJ, Jeong AR, Kim HJ, Lee N, Oh SW, Kim YJ, Chun HS, Koo MS. Microbiological quality of fresh-cut produce and organic vegetables. Korean J. Food Sci. Technol. 43: 91-97 (2011)
  19. Hausler WJ. Topley and Wilson's Microbiology and Microbial Infection 9th edition. vol. III, pp. 539-565. Hodder Arnold Pub., London, UK (1998)
  20. EFSA. Opinion of the Scientific Panel on Biological Hazards on Bacillus cereus and other Bacillus spp. In foodstuffs. European Food Safety Authority. Parma, Italy. pp. 1-48 (2005)
  21. Granum PE, Lund T. Bacillus cereus and its food poisoning toxins. FEMS microbiol. lett. 157: 223-228 (1997)
  22. Park SH, Park YK, Hwang IY, Park HY, Sung GH, Jo HC. Study on the characteristics of food-born pathogenes isolated from students' mobile phones in Busan. Envrion Health Sci. 42: 255-265 (2016)
  23. Ehling-Schulz M, Svensson B, Guinebretiere MH, Lindbck T, Andersson M, Schulz A, Fricher M, Christiansson A, Granum PE, Mrtibauer E, Nguyen-the C, Salkinoja-Salonen M, Scherer S. Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology 151: 183-197 (2005)
  24. Kim JH, Lim EG, Jang HC, Park JY, Lee SJ, Park MS, Choi GB, Lee BK. A case of emetic toxin producing Bacillus cereus strains isolated from outbreak. Korean J. Clin. Microbiol. 12: 48-52 (2009)
  25. Kim SK. A study of emetic toxin and enterotoxin producing B. cereus strains isolated from outbreak of Jeonbuk province. Chonbuk National University, Jeonju, Korea (2013)
  26. Kim SR, Lee JY, Lee SH, Ryu KY, Park KH, Kim BS, Yoon YH, Shim WB, Kim KY, Ha SD, Yun JC, Chung DH. Profiles of toxin genes and antibiotic susceptibility of Bacillus cereus isolated from perilla leaf and cultivation areas. Korean J. Food Sci. Technol. 43: 134-141 (2011)