Radioprotective effect of naringin and naringenin against cellular damage and oxidative stress of γ-irradiated mice

감마선을 조사한 마우스의 세포 손상과 산화적 스트레스에 대한 나린진과 나린제닌의 방사선방호 효과

  • Kang, Jung Ae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Hye Rim (Cancer Research Division, Research Institute, National Cancer Center) ;
  • Yoon, Seon Hye (Department of Radiation Biotechnology and Applied Radioisotope Science, Korea University of Science and Technology) ;
  • Jang, Beom-Su (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Dae Seong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Sang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 강정애 (한국원자력연구원 첨단방사선연구소) ;
  • 김혜림 (국립암센터 암중개연구과) ;
  • 윤선혜 (한국과학기술연합대학원대학교 방사선동위원소 응용 및 생명공학) ;
  • 장범수 (한국원자력연구원 첨단방사선연구소) ;
  • 최대성 (한국원자력연구원 첨단방사선연구소) ;
  • 박상현 (한국원자력연구원 첨단방사선연구소)
  • Received : 2017.06.30
  • Accepted : 2017.08.24
  • Published : 2017.12.31


The present study was designed to evaluate the antioxidant activity and radioprotective effects of Naringin and Naringenin in ${\gamma}$-irradiated mice. The antioxidant activity of Naringin and Naringenin was evaluated by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. Healthy female BALB/c mice were administered Naringin and Naringenin orally ($90{\mu}M/dose$ and $180{\mu}M/dose$) for 7 consecutive days prior to ${\gamma}$-irradiation (6 Gy). Naringenin displayed a much higher antioxidant activity in ABTS and FRAP than naringin. ${\gamma}$-irradiation resulted in cellular damage with decreased spleen and thymus indices and white blood cells (WBC) count. Additionally, ${\gamma}$-irradiation significantly increased lipid peroxidation and decreased the levels of antioxidant enzymes and glutathione (GSH) in the liver tissue. Strikingly, prior administration of Naringenin resulted in considerable prevention of these symptoms. Protection against ${\gamma}$-irradiation-induced cellular damage by Naringenin is likely due to its higher its antioxidant activity. Together, these results confirm that Naringenin is a potent antioxidant and radioprotector.


Supported by : 미래창조과학부


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