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Protective effects of Aruncus dioicus var. kamtschaticus extract against hyperglycemic-induced neurotoxicity

포도당 처리로 유도된 뇌신경세포 독성에 대한 눈개승마 추출물의 보호효과

  • Park, Su Bin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 박수빈 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 이욱 (국립산림과학원 특용자원연구과) ;
  • 강진용 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 박상현 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus).농업생명과학연구원)
  • Received : 2017.07.04
  • Accepted : 2017.07.31
  • Published : 2017.12.31

Abstract

To assess the physiological effects of Aruncus dioicus var. kamtschaticus extract on cytoxicity of a neuronal cell line, antioxidant activity, and neuroprotection against intensive glucose-induced oxidative stress were quantitated. Compared to the other fractions, the ethyl acetate fraction of Aruncus dioicus var. kamtschaticus (EFAD) showed the highest total phenolics and flavonoids. The 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay and malondialdehyde inhibitory effect test confirmed the superior antioxidant activity of EFAD. Moreover, EFAD also decreased the intracellular ROS level and suppressed neuronal cell death against intensive glucose- or $H_2O_2$-induced oxidative stress. Additionally, assessment of ${\alpha}$-glucosidase and acetylcholinesterase inhibitory activities revealed that EFAD was an effective inhibitor of ${\alpha}$-glucosidase and acetylcholinesterase. Finally, high-performance liquid chromatography analysis identified caffeic acid as the main ingredient of EFAD. Overall, these results suggest that the EFAD is a good natural source of biological compounds that counteract hyperglycemic neuronal defects.

Keywords

antioxidant activity;Aruncus dioicus var. kamtschaticus;caffeic acid;hyperglycemic neurotoxicity

Acknowledgement

Supported by : 산림청

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