- Volume 20 Issue 4
DOI QR Code
Quantitative Analyses of the Functional Constituents in SanYangSam and SanYangSanSam
- Shin, Il-Soo (East-West Cancer Center, Daejeon University) ;
- Jo, Eunbi (Division of Bioconvergence Analysis, Korea Basic Science Institute) ;
- Jang, Ik-Soon (Division of Bioconvergence Analysis, Korea Basic Science Institute) ;
- Yoo, Hwa-Seung (East-West Cancer Center, Daejeon University)
- Received : 2017.10.11
- Accepted : 2017.11.24
- Published : 2017.12.31
Objective: SanYangSam and SanYangSanSam are traditional Korea-medical herbs that are grown from Panax ginseng C.A. Meyer. In our previous studies, we found that the functional compounds in SanYangSam and SanYangSanSam were different and depended on the type and the cultivation environment of ginseng. This study aimed to profile the functional constituents in SanYangSam and SanYangSanSam. Methods: To profile the functional aspects of the many compounds that have therapeutic activities in SanYangSam and SanYangSanSam extracts, we used liquid chromatography tandem mass spectrometry and quadrupole orthogonal acceleration time-of-flight mass spectrometry. Results: A total of four major compounds were detected; two of which were the natural flavonoids kaempferol and quercetin. Among others, two polyacetylene compounds, including panaxydol and panaxynol, were detected. Conclusion: In this study, we found that panaxydol, one of the polyacetylene constituents of ginseng, is a candidate anti-cancer agent in SanYangSam and SanYangSanSam pharmacopuncture. In addition, we found that the panaxydol levels in the SanYangSanSam extract were over 30 times those in the SanYangSam extract.
Grant : A study on the programs to support collaborative research among industry, academia, and research institutes
Supported by : Korea Industrial Technology Association (KOITA)
- Park, J.G., et al., Anticancer effect of joboksansam, Korean wild ginseng germinated from bird feces. J Ginseng Res, 2016. 40(3): p. 304-8. https://doi.org/10.1016/j.jgr.2016.02.002
- Kim, H.J., et al., LC-MS-based metabolomic analysis of serum and livers from red ginseng-fed rats. J Ginseng Res, 2013. 37(3): p. 371-8. https://doi.org/10.5142/jgr.2013.37.371
- Kim, D., et al., Perspectives on the Market Globalization of Korean Herbal Manufacturers: A Company-Based Survey. Evid Based Complement Alternat Med, 2015. 2015: p. 515328.
- Wang, C.Z., et al., Red American ginseng: ginsenoside constituents and antiproliferative activities of heat-processed Panax quinquefolius roots. Planta Med, 2007. 73(7): p. 669-74. https://doi.org/10.1055/s-2007-981524
- Kim, Y.J., et al., Ginsenoside profiles and related gene expression during foliation in Panax ginseng Meyer. J Ginseng Res, 2014. 38(1): p. 66-72. https://doi.org/10.1016/j.jgr.2013.11.001
- Lee, Y.S., et al., Integrated Transcriptomic and Metabolomic Analysis of Five Panax ginseng Cultivars Reveals the Dynamics of Ginsenoside Biosynthesis. Front Plant Sci, 2017. 8: p. 1048. https://doi.org/10.3389/fpls.2017.01048
- Wan, J.Y., et al., Determination of American ginseng saponins and their metabolites in human plasma, urine and feces samples by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2016. 1015-1016: p. 62-73. https://doi.org/10.1016/j.jchromb.2016.02.008
- Kakarla, L., S.B. Katragadda, and M. Botlagunta, Morphological and Chemoprofile (Liquid Chromatography-mass Spectroscopy and Gas Chromatography-mass Spectroscopy) Comparisons of Cyperus scariosus R. Br and Cyperus rotundus L. Pharmacogn Mag, 2015. 11(Suppl 3): p. S439-47. https://doi.org/10.4103/0973-1296.168975
- Gu, H., et al., Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage. Anal Chem, 2015. 87(24): p. 12355-62. https://doi.org/10.1021/acs.analchem.5b03812
- Zhu, Z.J., et al., Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database. Nat Protoc, 2013. 8(3): p. 451-60. https://doi.org/10.1038/nprot.2013.004
- Danafar, H. and M. Hamidi, LC-MS Method for Studying the Pharmacokinetics and Bioequivalence of Clonidine Hydrochloride in Healthy Male Volunteers. Avicenna J Med Biotechnol, 2016. 8(2): p. 91-8.
- Dieuleveux, V., et al., Purification and characterization of anti-Listeria compounds produced by Geotrichum candidum. Appl Environ Microbiol, 1998. 64(2): p. 800-3.
- Khaksari, M., et al., Data representing two separate LCMS methods for detection and quantification of water- soluble and fat-soluble vitamins in tears and blood serum. Data Brief, 2017. 11: p. 316-330. https://doi.org/10.1016/j.dib.2017.02.033
- Winther, B., et al., Elucidation of phosphatidylcholine composition in krill oil extracted from Euphausia superba. Lipids, 2011. 46(1): p. 25-36. https://doi.org/10.1007/s11745-010-3472-6
- Li, J.J., et al., Analysis of Therapeutic Effect of Ilex hainanensis Merr. Extract on Nonalcoholic Fatty Liver Disease through Urine Metabolite Profiling by Ultraperformance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry. Evid Based Complement Alternat Med, 2013. 2013: p. 451975.
- Sun, M., et al., Alkaloid profiling of the traditional Chinese medicine Rhizoma corydalis using high performance liquid chromatography-tandem quadrupole time-of- flight mass spectrometry. Acta Pharm Sin B, 2014. 4(3): p. 208-16. https://doi.org/10.1016/j.apsb.2014.04.003
- Lee, J.H., et al., Advanced cancer cases treated with cultivated wild ginseng phamacopuncture. J Acupunct Meridian Stud, 2010. 3(2): p. 119-24. https://doi.org/10.1016/S2005-2901(10)60022-2
- Kwon, K.R., et al., Case series of non-small cell lung cancer treated with mountain Ginseng pharmacopuncture. J Acupunct Meridian Stud, 2011. 4(1): p. 61-8. https://doi.org/10.1016/S2005-2901(11)60008-3
- Yang, M.C., et al., Polyacetylenes from the roots of cultivated-wild ginseng and their cytotoxicity in vitro. Arch Pharm Res, 2008. 31(2): p. 154-9. https://doi.org/10.1007/s12272-001-1134-1
- Kim, H.S., et al., Panaxydol, a component of Panax ginseng, induces apoptosis in cancer cells through EGFR activation and ER stress and inhibits tumor growth in mouse models. Int J Cancer, 2016. 138(6): p. 1432-41. https://doi.org/10.1002/ijc.29879