Synergistic effect of phosphate solubilization by Burkholderia strains isolated from button mushroom bed

양송이배지로부터 분리한 Burkholderia균의 인산가용화 공조효과

  • Park, Ji-Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Han, Chang-Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 박지훈 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 한창훈 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경화학과)
  • Received : 2017.11.28
  • Accepted : 2017.12.21
  • Published : 2017.12.31


This study investigated the synergistic effect of single inoculation and co-inoculation of phosphate-solubilizing bacteria (PSB) Burkholderia metallica JH-7 and Burkholderia contaminans JH-15. Phosphate-solubilizing abilities of these strains were assessed by measuring phosphorus content in culture media that were singly inoculated or co-inoculated with these strains for 7 days. B. metallica JH-7 was found to release the highest content of soluble phosphorus ($140.80{\mu}g\;mL^{-1}$ ) into the medium, followed by single inoculation of B. contaminans JH-15 ($135.95{\mu}g\;mL^{-1}$ ) and co-inoculation of two strains ($134.84{\mu}g\;mL^{-1}$ ). The highest pH reduction, organic acid production, and glucose consumption were observed in the medium inoculated with B. metallica JH-7 alone compared with that in the medium co-inoculated with both the strains. Results of a plant growth promotion bioassay showed 17.4% and 7.48% higher leaf and root growth, respectively, in romaine lettuce inoculated with B. metallica JH-7 alone than in romaine lettuce inoculated with a control strain. However, no significant difference was observed between single inoculation and co-inoculation of these strains with respect to phosphorus release and plant growth. Although the results of the present study did not show the synergistic effect of phosphate solubilization by the PSB strains examined, these results indicate that treatment with PSB exerts a beneficial effect on crop growth.


Supported by : 충남대학교


  1. Ahuja A, Ghosh SB, D'Souza SF. 2007. Isolation of starch utilizing, phosphate solubilization fungus medium and its characterization. Bioresour Technol. 98:3408-3411.
  2. Bras, RR. and Nahas E. 2012. Synergistic action of both Aspergillus niger and Burkholderia cepacea in co-culture increases phosphate solubilization in growth medium. FEMS Microbiol. Lett. 332:84-90.
  3. Chaiharn M. and Lumyong S. 2009. Phosphate solubilization potential and stress tolerance of Rhizobacteria from rice soil in Nothern Thailand. World J Microbiol. Biotechnol.25:305-314.
  4. Egamberdieva D, Shrivastava S, Varma A. 2015. Plant-growth promoting rhizobacteria (PGPR) and medicinal plants. Soil Biology pp 3-4
  5. EI-Yazeid, AA and Abou-Aly HE. 2011. Enhancing growth, productivity and quality of tomato plants using phosphate solubilizing microorganisms. Australian J Basic Appl Sci. 5:371-379.
  6. Fernandez LA, Zalba P, Gomez MA, Sagardoy MA. 2007. Phosphate solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biol Ferti Soils 43:805-809.
  7. Khalimi, K, Suprapta DN, Nitta Y. 2012. Effect of Pantoea agglomerans on growth promotion and yield of rice. Agri Sci Res J. 2:240-249.
  8. Lee KK, Mok IK, Yoon MH, Kim HJ, Chung DY 2012. Mechanisms of Phosphate Solubilization by PSB (Phosphatesolubilizing Bacteria) in Soil. Korean J. Soil Sci. Fert. 45:169-176
  9. Mundra S, Arora R, Stobdan T. 2011. Solubilization of insoluble inorganic phosphates by a novel temperature, pH, and salt tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India. World J Microbiol Technol. 27:2387-2396.
  10. Park JH, Lee HH, Han CH, Yoo JA, Yoon MH. 2016. Synergistic effect of co-inoculation with phosphate solubilizing bacteria. Korean J. Soil Sci. Fert. 43:401-414
  11. Qureshi MA, Shakir MA, Iqbal A, Akhtar N, Khan K. 2011. Coinoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of mung bean (Vigna radiate L.). J Animal Plant Sci. 21:491-497.
  12. Reyes I, Bernier L, Antoun H. 2002. Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microbial Ecol. 44:39-48.
  13. Rodriguez H and Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv. 17:319-39
  14. Sane SA and Mehta SK. 2015 Isolation and evaluation of rock phosphate solubilizing fungi as potential biofertilizer. J. Ferti. Pestil. 6:2. DOI: 10.4172/2471-2728.1000156
  15. Suh JS and Kwon JS. 2008. Characterization of phosphatesolubilizing microorganisms in upland and plastic film house soils. Korean J Soil Sci Fert. 41:348-353
  16. Walpola BC and Yoon MH. 2013. Phosphate solubilizing bacteria: Isolation and assess their effect on growth promotion and phosphorous uptake of green gram plants (Vigna radiata [L.] R. Wilczek). Chillenean J Agri Res. 73: 275-281
  17. Walpola BC, Jang HJ, Yoon MH. 2013. Synergistic phosphate solubilization by Burkholderia anthina and Aspergillus awamori. Korean J Soil Sci Fert. 46:117-121