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Isolation and characterization of Bacillus subtilis NO12 from button mushroom substrates

양송이 배지로부터 분리된 Bacillus subtilis NO12의 특성

  • Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Park, Hyun Young (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Soo Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 박현영 (경남과학기술대학교 제약공학과) ;
  • 이찬중 (국립원예특작과학원 버섯과) ;
  • 공원식 (국립원예특작과학원 버섯과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Received : 2017.09.10
  • Accepted : 2017.11.27
  • Published : 2017.12.31

Abstract

Twelve strains of bacteria with cellulase and xylanase activities were isolated from spent mushroom substrates collected from button mushroom cultivation farm, Buye, Chungcheongnam-do in Korea. Among them, one strain, designated NO12, with higher cellulase and xylanase activities was selected by agar diffusion method. The strain NO12 was identified to be a Bacillus sp. by biochemical characteristics using Bacillus ID kit and MicroLog system. Comparative 16S rDNA gene sequence analysis showed that strain NO12 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99.2%. Based on its physiological properties, biochemical characteristics, and phylogenetic distinctiveness, strain NO12 was classified within the genus Bacillus, for which the name Bacillus subtilis NO12 was proposed. The cellulase and xylanase activities of B. subtilis NO12 were slightly increased according to bacterial population from exponential phase to stationary phase in the growth curve for B. subtilis NO12. The xylanase activity continuously increased from the beginning of the exponential phase and exhibited maximum activity in the middle of the exponential phase.

Keywords

Bacillus subtilis NO12;Cellulase;Spent mushroom substrates;Xylanase

Acknowledgement

Supported by : 농촌진흥청

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