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Polymorphism of inter simple sequence repeat markers in Hypsizygus marmoreus

Inter Simple Sequence Repeat(ISSR) 마커를 활용한 느티만가닥버섯(Hypsizigus marmoreus) 종내 다형성 분석

  • Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Nam, Yun-Geul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Min ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Im, Ji-Hoon (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, In-Geol (Department of Biotechnology College of Life Sciences and Biotechnology Korea University)
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 남윤걸 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오민지 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 임지훈 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 최인걸 (고려대학교 생명공학과 대학원 생명공학과&BK21플러스)
  • Received : 2017.09.04
  • Accepted : 2017.11.27
  • Published : 2017.12.31

Abstract

Hypsizygus marmoreus is a mushroom with abundant flavor and medicinal properties. However, its application is limited by problems such as long cultivation period, low biological efficiency, and microbiological contamination; therefore, there is a substantial need for development of new cultivars of this species. In this study, 55 strains of H. marmoreus were subjected to inter simple sequence repeat (ISSR) analysis to identify markers for the selection of mother strains for breeding from the collected germplasm. ISSR 13 and 15 were confirmed as polymorphic markers. The three strains (KMCC03106, KMCC03107, and KMCC03108) with white cap color were found to be genetically closely related upon UPGMA analysis of both ISSR 13 and 15. Based on the PCR analysis results for ISSR 15, the collected germplasm were differentiated into three groups according to the strain collection year. Thus, ISSR 15 could be a marker for determining the phylogeny of cap color and genetic variations according to the strain collection year. These results suggest that ISSR markers can be effective tools for the selection of mother strains for breeding of H. marmoreus.

Keywords

Genetic polymorphism;Hypsizygus marmoreus;ISSR marker;UPGMA

Acknowledgement

Supported by : 농촌진흥청

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