Effects of Bangkibokryeong-tang (Fangjifuling-tang) on the Reduction of Blood Glucose and Body Fat in High Fat Diet Induced Obese Mice

방기복령탕(防己茯嶺湯)이 비만유도생쥐의 혈당 및 체지방개선에 미치는 영향

  • Received : 2016.12.27
  • Accepted : 2017.01.16
  • Published : 2017.01.31


Objectives This study aimed to investigate the effect of Bangkibokryeong-tang (BBT, Fangjifuling-tang) on blood glucose and body fat in high-fat diet-induced obese mice. Methods The experimental animals were divided into five groups- normal diet-fed control (ND), high-fat diet-fed control (HFD), HFD+BBT 75, HFD+BBT 150, and HFD+olistat as a positive drug control group. Markers of obesity, such as body weight, organ weight, diet efficiency, and serum levels of total cholesterol, triglycerides, lipid content, leptin, adiponectin, glutamic oxaloacetic transaminase (GOT)/glutamic pyruvic transferase (GPT)/lactate dehydrogenase (LDH), blood glucose, and insulin, were measured. Furthermore, results of the oral glucose tolerance test and ${\alpha}-glucosidase$ inhibition activity were examined in obese mice. Results Mice treated with BBT demonstrate lower body and organ weight, and reduced weight gain and food efficiency than that in the HFD-only control group. In addition, BBT decreased lipid accumulation in the liver and the levels of enzymes such as GOT, GPT, and LDH in the serum. Furthermore, the levels of triglycerides, total cholesterol, low density lipoprotein (LDL), and leptin were decreased in the serum but the levels of high density lipoprotein (HDL) and adiponectin were increased in the BBT-treated group compared with the control group. The BBT-treated group also demonstrated decreased blood glucose and insulin concentrations induced by feeding on a high-fat diet and improved glucose tolerance. Conclusions Based on the results above, BBT may reduce body fat and hyperglycemia in HFD-induced obesity. This suggests that BBT may be clinically useful in the treatment of obesity.


Bangkibokryeong-tang;Obesity;High fat diet;Hyperglycemia


  1. The Society of Korean Medicine Rehabilitation. Korean medicine rehabilitation. 3rd ed. Seoul : Koonja Publishing Inc. 2011:350.
  2. Korean society for the study of obesity. Clinical bariatrics. Seoul : Korean medicine. 2011 : 1-26.
  3. Korea National Health and Nutrition Examination Survey. 2014.
  4. Press release of National health insurance 2014. Retrieved Dec. 14, 2015. http : //
  5. Bjorntorp P. The associations between obesity, adipose tissue distribution and disease. Acta Med Scand Suppl. 1988;723:121-34.
  6. Visscher TL. The public health impact of obesity. Annu Rev Pub Health. 2001;22:355-75.
  7. Lee HK. Diseases associated with obesity. J Nut Health. 1990;23(5):341-6.
  8. Yang KM. Relationship between the assessment of obesity and cardiovascular diseases. JKFN. 2002;7(2):1-8.
  9. Yoon YS, Keum SO, Lee SW, Kim IH, Lee HI, Song YS. Effects of Cheongshimyeonja-tang on body weight and serum lipid levels in high fat diet induced obese mice. Herb Formula Sci. 2014;22(1):123-39.
  10. Korea Institute of Oriental Medicine. Korean Medicine Clinical Practice Guideline - Obesity. Seoul : Elsevier Korea LLC. 2016 : 35-6.
  11. The Society of Korean Medicine Rehabilitation. Korean medicine rehabilitation. 4th ed. Seoul : Koonja Publishing Inc. 2015:298-317.
  12. Jeong JW. Trend research of the human body-oriented obesity studies on oriental medicine. [Master's thesis]. Busan : Dong-Eui University. 2016. Korean Language.
  13. 李尙仁. 方劑學. Seoul : Younglim Publishing Inc. 1990:287-9,291-2.
  14. 張仲景. 金櫃要略方論. 臺北 : 臺聯國風出版社. 1995:70.
  15. Ghee SS, Lee YJ. The therapeutic effects of Bangkeehwangkee-tang and Bangkeebokryeong-tang on the hyperlipidemia in rats. Kor. J. Herbol. 2005;20(2):149-57.
  16. Ghee SS. The study on uses of Bangkee in Bangkeehwangkee-tang and Bangkeebokryeong-tang. [Doctorate thesis]. Seongnam : Gachon University. 2003. Korean Language.
  17. Im LH. The Effects of Bangiboklyengtang and Worlbikachultang on rats with nephrosis induced by puromycin amininucleoside. [Doctorate thesis]. Seoul : Kyunghee University. 2001. Korean Language.
  18. Song JB, Ryu DG, Lee UJ. The Effect of Banggibokryeongtang Water Extract on Kidney function in Rat. HFS. 1990;1(1):147-62.
  19. Folch J, Lees M, Sloane-Stanley G. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957;226(1):497-509.
  20. Frings CS, Dunn RT. A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. Am J Clin Pathol. 1970;53(1):89-91.
  21. Zobel EH, Hansen TW, Rossing P, von Scholten BJ. Global Changes in Food Supply and the Obesity Epidemic. Curr Obes Rep. 2016. 30. [Epub ahead of print]
  22. Press release of National health insurance 2014. Retrieved Dec. 14, 2015. http : //
  23. Kim YS. Clinical stroke. Seoul : Seowondang. 1997 : 347-438.
  24. Press release of World Health Organization. Retrieved Dec. 14, 2015. from : http : // /fs311/en/.
  25. Kim KS, Park SW. Drug therapy for obesity. Korean J Obes. 2012;21(4):197-202.
  26. Kim SG, An GH, Yoon SW, Lee YC, Ha SD. A study on dietary supplement to reduce obesity by the mechanism of decreasing lipid and carbohydrate absorption. Korean J Food Sci Technol. 2003;35(3):519-26.
  27. Jin KS. Analysis of the tendency to research for obesity. MS Thesis. Daejeon: Graduate School of Daejeon University. 2004.
  28. Gang SA, Jang GH, Park SG, Lim JP, Jeon H, Choi H, et al. Effects of herbal compositionon obese a high fat diet. Kor J Herbol. 2003;18(4):59-64.
  29. Song YS, Lee MJ, Jeong SH, Lee JS, Kim SS, Shin HD. Effects of Bangkihwangkitang, Bangkihwangkitang with Lycii fructus and Lycii fructus on the weight of obese rats. J Orient Rehab Med. 1991;1(1):25-43.
  30. Lee ES, Kim SS, Jeong SH, Lee JS, Shin HD. Effects of Bangkihwangkitang on adipose tissue of liver and epididymis and serum lipid. J Orient Rehab Med. 1995; 5(1):1-10.
  31. Hwang MJ, Shin HS, Song MY. Review of literature on herbal medicines for the treatment of obesity in Korea: mainly papers since 2000. Orient Rehab Med. 2006;16(3):65-81.
  32. 黃度淵. 新增 方藥合編. Seoul : Younglim Publishing Inc. 2011:377-80,485-6,497-8,532-3.
  33. KIM HS, Zhang YH, Fang LH, Yun YP, Lee HK. Effects of tetrandrine and fangchinoline on human platelet aggregation and thromboxane B 2 formation. Journal of Ethnopharmacology. 1999;66(2):241-6.
  34. Huang YT, Chang FC, Chen KJ, Hong CY. Acute hemodynamic effects of tetramethylpyrazine and tetrandrine on cirrhotic rats. Planta Medica. 1999;65(2):130-4.
  35. WK Seow, A Ferrante, DB Goh, AH Chalmers, SY Li, YH Thong. In vitro immunosuppressive properties of the plant alkaloid tetrandrine. International archives of allergy and applied immunology. 1988;85(4):410-5.
  36. Kim H M, Moon PD, Chae HJ, Kim HR, Chung JG, Kim JJ, Lee EJ. The stem of Sinomenium acutum inhibits mast cell-mediated anaphylactic reactions and tumor necrosis factor-${\alpha}$ production from rat peritoneal mast cells. Journal of Ethnopharmacology. 2000;70(2):135-41.
  37. JH Sampson, JD Phillipson, NG Bowery, MJ O'Neill, JG Houston, JA Lewis. Ethnomedicinally selected plants as sources of potential analgesic compounds: indication of in vitro biological activity in receptor binding assays. Phytotherapy Research. 2000;14(1):24-9.<24::AID-PTR537>3.0.CO;2-9
  38. D Candinas, W Mark, V Kaever, T Miyatake, N Koyamada, P Hechenleitner, WW Hancock. Immunomodulatory effects of the alkaloid sinomenine in the high responder ACI-to-Lewis cardiac allograft model. TRANSPLANTATION. 1996;62(12):1855-915.
  39. L Liu, J Riese, K Resch, V Kaever. Impairment of macrophage eicosanoid and nitric oxide production by an alkaloid from Sinomenium acutum. Arzneimittelforschung. 1994;44(11):1223-9.
  40. Jeong CG. Study on the Effects of Astragali Radix, Cinnamomi Ramulus and Carthami Flos on Thrombosis and Elevated Blood Viscosity. [Doctorate thesis]. Seoul : Kyunghee University. 2001. Korean Language.
  41. M Sato, T Tai, Y Nunoura, Y Yajima, S Kawashima, K Tanaka. Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin. Biological and Pharmaceutical Bulletin. 2002;25(1):81-6.
  42. T Kaminaga, K Yasukawa, H Kanno, T Tai, Y Nunoura, M Takido. Inhibitory effects of lanostane-type triterpene acids, the components of Poria cocos, on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin. ONCOLOGY. 1996;53(5):382-7.
  43. H Nukaya, H Yamashiro, H Fukazawa, H Ishida, K Tsuji. Isolation of Inhibitors of TPA-Induced Mouse Ear Edema from Hoelen, Poria cocos. Chemical and Pharmaceutical Bulletin. 1996;44(4):847-9.
  44. Wang CY, James KL. A Mouse Model of Diet-Induced Obesity and Insulin Resistance. Methods Mol Biol. 2012;821:421-33.
  45. Kareem Hassan, Varun Bhalla, Mohammed Ezz El Regal, H Hesham A-Kader. Nonalcoholic fatty liver disease: A comprehensive review of a growing epidemic. World J Gastroenterol. 2014;220:12082-101.
  46. Stranges S, Dorn JM, Muti P, Freudenheim JL, Farinaro E, Russell M, Nochajski TH, Trevisan M. Body fat distribution, relative weight, and liver enzyme levels: a population-based study. Hepatology. 2004;39:754-63.
  47. Kannel WB, Wilson PWF. An update on coronary risk factors. Med Clin North Am. 1995;79:951-6.
  48. Tomkin GH. Atherosclerosis, diabetes and lipoproteins. Expert Rev Cardiovasc Ther. 2010;8:1015-29.
  49. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE, Tataranni PA. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab. 2001;86:1930-5.
  50. Fasshauer M, Paschke R. Regulation of adipokines and insulin resistance. Diabetologia. 2003;46:1594-603.
  51. Martyn JA, Kaneki M, Yasuhara S. Obesity-induced insulin resistance and hyperglycemia: etiologic factors and molecular mechanisms. Anesthesiology. 2008;109:137-48.
  52. Matsuzawa Y, Fanahashi T, Nakamura T. Moleculare mechanism of metabolic syndrome X: contribution of adiposcyte-derived bioactive substances. Annals of the New York Academy of Sciences. 1999;892:146-54.


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