DOI QR코드

DOI QR Code

Comparison of Index Compound Content and Anti-inflammatory Activity of Eucommiae Cortex by Various Drying Methods

두충의 건조법별 지표성분 및 항염증 활성 비교

  • Received : 2016.12.20
  • Accepted : 2017.01.03
  • Published : 2017.01.31

Abstract

Objectives Eucommiae cortex is one of the frequently used herbs for musculoskeletal disorders, with well-known anti-inflammatory effect. Meanwhile, powdered form of herbal drugs is more advantageous than decoction form, in that storing and taking is more convenient. Thus, the authors aimed to investigate whether the drying method affects the index compound level and anti-inflammatory effect of eucommiae cortex water extract. Methods Eucommiae cortex was extracted in distilled water at $100^{\circ}C$ for 3 hours, filtered, and then evaporated under vacuum. One half of the sample was freeze-dried at $-80^{\circ}C$. Another half of the sample was added with dextrin and then spray-dried at $180^{\circ}C$. To assess the possible change in index compound content, pinoresinol diglucoside was selected as the index marker. The content level of the index compound in various extract sample was quantified through high performance liquid chromatography. In addition, iNOS assay in LPS-induced RAW 264.7 cells was adopted to determine the anti-inflammatory effect of various extract samples. Furthermore, MTT assay was performed to confirm that the result of iNOS assay was not due to cytotoxicity. Results There was no significance difference in index compound content between extract samples obtained through two different drying methods. Anti-inflammatory activity of extract samples were similar at the matching concentration, regardless of the drying methods. Extract samples did not show any significant cytotoxicity. Conclusions Extract samples of eucommiae cortex were obtained through freeze-drying and spray-drying. Neither change in index compound content nor difference in anti-inflammatory activity was observed between drying methods.

Keywords

Eucommiae cortex;Pinoresinol diglucoside;Freeze-drying;Spray-drying;Anti-inflammatory

References

  1. Choi H, Choi J, Lee CH. The chart of herbal medicine in Korean. 1st ed. Paju:Jipmoondang. 2010:191-2.
  2. Jeong YS, Moon BK, Nam HW, Park JS, Eum SY, Choe BM. The Survey on Using Alternative Medicine in General Population with Medical Problems : A Pilot Study. Korean J Psychosomatic Medicine. 1998;6(1):70-8.
  3. Research institute of east oriental medicine. Introduction to east oriental medicine. Seoul:Rao-gang. 1994:333.
  4. Choi HJ, Bang NY, Song BW, Kim NJ, Ryu BH. Survey on the preference for the dosage forms of oriental herbal medicine. J Kyung Hee Univ Med Cent. 2004;20(1):356-67.
  5. Ahn DG. Mysterious treatment of eucommia ulmoides, Paju:Openbooks, 1993.
  6. Research institute of east oriental medicine. Herbology. Seoul:Ryo-gang, 1994.
  7. Chien TH. Pharmacological action of Eucommia ulmoides. Japanese Journal of Pharmacology. 1957;6(2):122-37. https://doi.org/10.1254/jjp.6.122
  8. Kin KC, Ting KS. Studies on drugs for treatment of hypertension: II. Toxicity and experimental therapy of Eucommia ulmoides Oliv. Acta Physiologica Sinica. 1956;20:247-54.
  9. Au C, Yang CP, Luang CJ, Leu HJ. The diuretic and cardiovascular effects of cortex eucommiae. Bull Taipei Med Coll. 1986.
  10. Sih CJ, Ravikumar PR, Huang FC, Buckner C, Whitlock H. Isolation and synthesis of pinoresinol diglucoside a major antihypertensive principle of Tu-Chung(Eucommia ulmoides). Journal of the American Chemical Society. 1976;98(17):5412-5. https://doi.org/10.1021/ja00433a070
  11. Xu SL, Zeng QZ, Huang WG, Yin Q. Effects of Duzhong on plasma cAMP and cGMP levels in mice. Chinese Traditional and Herbal Drugs. 1986;17:204.
  12. Nakamura T, Nakazawa Y, Onizuka S. Antimutagenicity of Tochu tea(an aqueous of Eucomia ulmoides leaves): 1. The clastogen- suppressing effects of Tochu tea in CHO cell and mice. Mutation Research. 1997;388(1);7-20. https://doi.org/10.1016/S1383-5718(96)00096-4
  13. Man SC, Durairajan SSK, Kum WF, Lu JH, Huang JD, Cheng CF, Chung V, Xu M, Li M. Systematic review on the efficacy and safety of herbal medicines for Alzheimer's disease. Journal of Alzheimer's disease. 2008;14(2):209-23. https://doi.org/10.3233/JAD-2008-14209
  14. Park IH, Kim YH, Choi SH, Yu SN, Kim SH, Ahn SC, Cho SI, Lee I. Effect of Preservation Conditions on the Stability of Samul-tang Decoctions. Journal of Life Science. 2015;25(10):1124-31. https://doi.org/10.5352/JLS.2015.25.10.1124
  15. Yoon SJ, Lee SI. Study of Jagyakgamchotang's effect change by time. J of Herbology. 1991;6(1):29-34.
  16. Jin SE, Kim OS, Seo CS, Shin HK, Jeong SJ. Comparative study on stability and efficacy of Banhasasim-tang decoction depending on the preservation temperature and period. Journal of Korean Medicine. 2016;37(1):21-33. https://doi.org/10.13048/jkm.16003
  17. Jin SE, Kim OS, Shin HK, Jeong SJ. Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods. Journal of Korean Medicine. 2014;35(3):60-9.
  18. Tsami E, Krokida MK, Drouzas AE. Effect of drying method on the sorption chracteristics of medel fruit powder. Journal of food engineering. 1998;38(4):381-92. https://doi.org/10.1016/S0260-8774(98)00130-7
  19. Kailasapathy K. Microencapsulation of probiotic bacteria: technology and potential applications. Curr Issues Intest Microbiol. 2002;3(2):39-48.
  20. Cho YH, Shin DS, Park JY. Microencapsulation technology in food industry. Food Science and Industry. 1997;30:98-111.
  21. Sano Y. Gas flow behaviour in spray dryer. Drying Technol. 1993;11(4):697-718. https://doi.org/10.1080/07373939308916859
  22. Frank F. Freezing-drying of bioproducts; putting princicples into practice. European Journal of Pharmaceutics and Biopharmaceutics. 1997;45:221-9.
  23. Jafar F, Farid M. Analysis of Heat and Mass transfer in freeze drying. Drying technology. 2003;21(2):249-63. https://doi.org/10.1081/DRT-120017746
  24. Hammami C, Rene F. Determination of freezing-drying process variables for strawberries. Journal of Food engineering. 1997;32(2)133-54. https://doi.org/10.1016/S0260-8774(97)00023-X
  25. Erbay Z, Icier F. Optimization of hot air drying of olive leaves using response surface methodology. Journal of food engineering. 2009;91(4):533-41. https://doi.org/10.1016/j.jfoodeng.2008.10.004
  26. Seo CS, Kim JH, Shin HK, Kim BS. Quantitative Determination of the Six Marker Compounds in Eucommiae Cortex by Processing Method. Korean Journal of Pharmacognosy. 2015;46(2):123-32.
  27. Chen XY, Luo LL, Ren GC, Qu GY, Dong LS. Determination of pinoresinol diglucoside in processed Eucommia ulmoides and crude Eucommia ulmoides. West China Journal of Pharmaceutical Sciences. 2008;23(5):592-3.
  28. Park SD, Kim GW. Experimental studies of Eucommiae Cortex according to processing. The Journal of Dong Guk Oriental Medicine. 1992;1(1):81-107.
  29. Kim HK. Studies for standardization of processed herbal medicines(I). Seoul:Ministry of Food and Drug Safety. 2003;31-56.
  30. Oh HS, Kim HC, Lee SI, Ahn DK. Effects of Eucommiae Cortex and Folium on the Ovariectomized Rat as the Model of Postmenopaual Osteoporosis. Journal of Herbology. 1995;10(1):59-68.
  31. Lee JA, Noh SH, Ahn DK, Choi HY. Effects of the Eucommiae Cortex and Chanenomelis Fructus on the Aged Ovariectomized Rat of Postmenopausal Osteoporosis. Journal of Herbology. 2001;16(1):201-6.
  32. Ha HY, Ho JY, Shin SM, Kim HJ, Koo SJ, Kim IH, Kim CS. Effects of Eucommiae Cortex on osteoblast-like cell proliferation and osteoclast inhibition. Archives of Pharmacal Research. 2003;26(11):929-36. https://doi.org/10.1007/BF02980202
  33. Jang JB, Kim KJ, Choi GY, Cho JH, Park YK, Lee KS. The Effects of Eucommiae Cortex on Bone Histomorphologic Change in the Ovariectomized Rats. The Journal of oriental obstetrics and gynecology. 2003;16(4):1-14.
  34. Li Y, Kamo S, Metori K, Koike K, Che QM, Takahashi S. The promoting effect of eucommiol from Eucommiae cortex on collagen synthesis. Biological and Pharmaceutical Bulletin. 2000;23(1):54-9. https://doi.org/10.1248/bpb.23.54
  35. Kim UJ, Kim SH. Effect of Eucommia Cortex and Eucommia Cortex Leaf on Immune Function. Journal of Herbology. 1994;9(1):99-113.
  36. Lee CG, Lee BK. Effect of Eucommia Cortex and Eucommia Cortex Leaf on the Development of Experimental Hypercholesterolemia in the Rats. The Journal of East-West Medicines. 1987;12(2):17-31.
  37. Kim JB, Park JR, Jeon JR, Cha MH. Isolation and Identification of Anticancer Compounds from Eucommia ulmoides Leaves. Journal of the Korean Society of Food Science and Nutrition. 2001;30(4):732-8.
  38. Cho JH, Kim KS, Cha JD, Lee HS, Choi H, Jung HS, Sohn NW, Sohn YJ. Effect of Eucommiae Cortex on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats. Korean J. Oriental Physiology & Pathology. 2008;22(6):1454-61.