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In Vitro and In Vivo Anti-Oxidative and Anti-Inflammatory Activities of Acer tegmentosum Maxim Extracts

RAW 264.7 대식세포와 염증유도 동물모델에서 산겨릅나무 추출물의 항산화 및 항염증 효과

  • Lee, Cho-Eun (Department of Food and Nutrition, Chungnam National University) ;
  • Jeong, Hyeon-Hee (Department of Food and Nutrition, Chungnam National University) ;
  • Cho, Jin-Ah (Department of Food and Nutrition, Chungnam National University) ;
  • Ly, Sun Yung (Department of Food and Nutrition, Chungnam National University)
  • 이초은 (충남대학교 식품영양학과) ;
  • 정현희 (충남대학교 식품영양학과) ;
  • 조진아 (충남대학교 식품영양학과) ;
  • 이선영 (충남대학교 식품영양학과)
  • Received : 2016.09.22
  • Accepted : 2016.10.28
  • Published : 2017.01.31

Abstract

Acer tegmentosum Maxim (ATM) is known as traditional medicine for treatment of hepatic disorders such as hepatitis, related-inflammatory disease, and hepatic cancer. In this study, we evaluated the antioxidant and anti-inflammatory effects of ATM extracted with $80^{\circ}C$ water or 95% ethanol. Antioxidant activities of ATM extracts were measured based on DPPH and ABTS radical scavenging activities, total polyphenolic compound contents, and ferric reducing antioxidant power. The anti-inflammatory effects of ATM extract were assayed on release of nitric oxide, tumor necrosis factor $(TNF)-{\alpha}$, and interferon $(IFN)-{\gamma}$ from lipopolysaccharide (LPS)-induced macrophages. In these experiments, 95% ethanol extract of ATM showed stronger antioxidant and anti-inflammatory effects than water extract. Therefore, we determined the effects of ATM ethanol extract on an animal model of sepsis. Seven days oral gavage of ATM ethanol extract followed by LPS stimulation reduced the protein levels of $TNF-{\alpha}$ and $IFN-{\gamma}$ in serum as well as mRNA levels of $TNF-{\alpha}$ and interleukin-6 in intestinal epithelial cells. In addition, ATM ethanol extract reduced DNA damage in mouse lymphocytes. These results indicate that ATM extract has strong antioxidant and anti-inflammatory in vitro and in vivo effects and may be developed as a potential food material for prevention of inflammatory diseases.

Acknowledgement

Supported by : 충남대학교

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