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RAW 264.7 대식세포와 염증유도 동물모델에서 산겨릅나무 추출물의 항산화 및 항염증 효과

In Vitro and In Vivo Anti-Oxidative and Anti-Inflammatory Activities of Acer tegmentosum Maxim Extracts

  • 이초은 (충남대학교 식품영양학과) ;
  • 정현희 (충남대학교 식품영양학과) ;
  • 조진아 (충남대학교 식품영양학과) ;
  • 이선영 (충남대학교 식품영양학과)
  • Lee, Cho-Eun (Department of Food and Nutrition, Chungnam National University) ;
  • Jeong, Hyeon-Hee (Department of Food and Nutrition, Chungnam National University) ;
  • Cho, Jin-Ah (Department of Food and Nutrition, Chungnam National University) ;
  • Ly, Sun Yung (Department of Food and Nutrition, Chungnam National University)
  • 투고 : 2016.09.22
  • 심사 : 2016.10.28
  • 발행 : 2017.01.31

초록

산겨릅나무 $80^{\circ}C$ 열수 및 95% 에탄올 추출물을 제조하여 항산화 및 항염증 효과를 확인한 결과 추출물은 우수한 항산화 효과를 나타내었고 RAW 264.7 세포에서도 항염증 효과를 보였으며, 그중 95% 에탄올 추출물의 효능이 더 큰 것으로 나타났다. 따라서 효능이 더 뛰어난 산겨릅나무 에탄올 추출물을 이용하여 in vivo 실험에서 추출물의 세포 보호효과 및 항염증 효능을 재확인하였다. ICR mouse에 일주일간 100 mg/kg B.W 산겨릅나무 에탄올 추출물을 경구투여한 후 LPS를 처리하여 염증반응 및 산화적 스트레스를 유도시켜 Comet assay, serum tumor necrosis $factor(TNF)-{\alpha}$$interferon-{\gamma}$, 장점막세포에서의 $TNF-{\alpha}$와 interleukin-6에 대한 생성능을 측정한 결과 산겨릅나무 에탄올 추출물은 DNA 손상을 억제하였으며 사이토카인 생성을 유의적으로 억제하여 항산화와 항염증 효과를 보였다. 따라서 본 연구에서 사용한 산겨릅나무의 에탄올 추출물은 산화적 스트레스 및 염증을 억제하는 효과를 나타내는 세포보호 기능적 소재로서 가능성이 있는 것으로 생각한다.

과제정보

연구 과제 주관 기관 : 충남대학교

참고문헌

  1. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  2. Halliwell B, Gutteridge J. 1999. Free radicals in biology and medicine. Oxford University Press Co., New York, NY, USA. р 968.
  3. Olefsky JM, Glass CK. 2010. Macrophages, inflammation, and insulin resistance. Annu Rev Physiol 72: 219-246. https://doi.org/10.1146/annurev-physiol-021909-135846
  4. Valledor AF, Comalada M, Santamaría-Babi LF, Lloberas J, Celada A. 2010. Macrophage proinflammatory activation and deactivation: a question of balance. Adv Immunol 108: 1-20.
  5. Jung SH, Kim SJ, Jun BG, Lee KT, Hong SP, Oh MS, Jang DS, Choi JH. 2013. $\alpha$-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and $PGE_2$ production through the negative regulation of $NF{\kappa}B$ signalling in RAW 264.7 cells. J Ethnopharmacol 147: 208-214. https://doi.org/10.1016/j.jep.2013.02.034
  6. Yu A, Park HY, Kim YS, Ha SK, Hong HD, Choi HD. 2012. Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J Korean Soc Food Sci Nutr 41: 1671-1676. https://doi.org/10.3746/jkfn.2012.41.12.1671
  7. Shan J, Fu J, Zhao Z, Kong X, Huang H, Luo L, Yin Z. 2009. Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing $NF-{\kappa}B$ and JNK/AP-1 activation. Int Immunopharmacol 9: 1042-1048. https://doi.org/10.1016/j.intimp.2009.04.011
  8. Lee EJ, Kim C, Kim JY, Kim SM, Nam D, Jang HJ, Kim SH, Shim BS, Ahn KS, Choi SH, Jung SH, Ahn KS. 2012. Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of $NF-{\kappa}B$ activation in RAW 264.7 cells. Immunopharmacol Immunotoxicol 34: 282-291. https://doi.org/10.3109/08923973.2011.602412
  9. Kim T. 1996. The woody plants of Korea in color. Kyo-Hak Publishing Co., Seoul, Korea. p 476.
  10. Park KM, Yang MC, Lee KH, Kim KR, Choi SU, Lee KR. 2006. Cytotoxic phenolic constituents of Acer tegmentosum maxim. Arch Pharm Res 29: 1086-1090. https://doi.org/10.1007/BF02969296
  11. Lee S, Moon G. 2010. Effects of mixture with Hovenia dulcis Thunb and Acer tegmentosum Maxim on liver failure induced by D-galactosamine in rats. J Korean Orient Int Med 31: 11-24.
  12. Lee SB, Woo HJ. 2010. A study of the inhibitory effect of Acer tegmentosum Max. on fibrogenesis in hepatic stellate cell line T6. J Korean Orient Int Med 31: 346-355.
  13. Yu T, Lee J, Lee YG, Byeon SE, Kim MH, Sohn EH, Lee YJ, Lee SG, Cho JY. 2010. In vitro and in vivo anti-inflammatory effects of ethanol extract from Acer tegmentosum. J Ethnopharmacol 128: 139-147. https://doi.org/10.1016/j.jep.2009.12.042
  14. Tung NH, Ding Y, Kim SK, Bae K, Kim YH. 2008. Total peroxyl radical-scavenging capacity of the chemical components from the stems of Acer tegmentosum Maxim. J Agric Food Chem 56: 10510-10514. https://doi.org/10.1021/jf8020283
  15. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  16. Pellegrini N, Re R, Yang M, Rice-Evans C. 1999. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay. Methods Enzymol 299: 379-389.
  17. Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239: 70-76. https://doi.org/10.1006/abio.1996.0292
  18. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
  19. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35: 206-221. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
  20. Cho EK, Jung KI, Choi YJ. 2015. Anti-diabetic, alcohol metabolizing enzyme and hepatoprotective activity of Acer tegmentosum Maxim. stem extracts. J Korean Soc Food Sci Nutr 44: 1785-1792. https://doi.org/10.3746/jkfn.2015.44.12.1785
  21. Wang M, Li J, Rangarajan M, Shao Y, LaVoie EJ, Huang TC, Ho CT. 1998. Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric Food Chem 46: 4869-4873. https://doi.org/10.1021/jf980614b
  22. Lee HR, Jung BR, Park JY, Hwang IW, Kim SK, Choi JU, Lee SH, Chung SK. 2008. Antioxidant activity and total phenolic contents of grape juice products in the Korean market. Korean J Food Preserv 15: 445-449.
  23. Arano MB, Cano A, Acosta M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73: 239-244. https://doi.org/10.1016/S0308-8146(00)00324-1
  24. Sanchez-Gonzalez I, Jimenez-Escrig A, Saura-Calixto F. 2005. In vitro antioxidant activity of coffees brewed using different procedures (Italian, espresso and filter). Food Chem 90: 133-139. https://doi.org/10.1016/j.foodchem.2004.03.037
  25. Choi JH, Lee SH, Park YH, Lee SG, Jung YT, Lee IS, Park JH, Kim HJ. 2013. Antioxidant and alcohol degradation activities of extracts from Acer tegmentosum Maxim.. J Korean Soc Food Sci Nutr 42: 378-383. https://doi.org/10.3746/jkfn.2013.42.3.378
  26. Han SS, Lo SC, Choi YH, Kim MJ, Kwak SS. 1999. Antioxidative compounds in extracts of Acer ginnala Max. J Crop Sci Biotechnol 7: 51-57.
  27. Lee HJ, Lee CW, Choi MS, Son DJ, Hong JT. 2006. Effects of esthetic essential oils on LPS-induced nitric oxide generation in murine marcrophage RAW 264.7 cells. J Soc Cosmet Scientists Korea 32: 111-116.
  28. Yoshitake J, Kato K, Yoshioka D, Sueishi Y, Sawa T, Akaike T, Yoshimura T. 2008. Suppression of NO production and 8-nitroguanosine formation by phenol-containing endocrinedisrupting chemicals in LPS-stimulated macrophages: involvement of estrogen receptor-dependent or -independent pathways. Nitric Oxide 18: 223-228. https://doi.org/10.1016/j.niox.2008.01.003
  29. Beutler BA, Milsark IW, Cerami A. 1985. Cachectin/tumor necrosis factor: production, distribution, and metabolic fate in vivo. J Immunol 135: 3972-3977.
  30. Beutler B, Cerami A. 1987. Cachectin: more than a tumor necrosis factor. N Engl J Med 316: 379-385. https://doi.org/10.1056/NEJM198702123160705
  31. Hayward AR, Chmura K, Cosyns M. 2000. Interferon-gamma is required for innate immunity to Cryptosporidium parvum in mice. J Infect Dis 182: 1001-1004. https://doi.org/10.1086/315802
  32. Jin BM, Lee GH, Hyun KY. 2014. Antidepressant effect of Acer tegmentosum Maxim on forced swimming test in the rat. J KAIS 15: 6739-6745.
  33. Vane JR, Mitchell JA, Appleton I, Tomlinson A, Bishop-Bailey D, Croxtall J, Willoughby DA. 1994. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc Natl Acad Sci U S A 91: 2046-2050. https://doi.org/10.1073/pnas.91.6.2046
  34. Ji G, Zhang Y, Yang Q, Cheng S, Hao J, Zhao X, Jiang Z. 2012. Genistein suppresses LPS-induced inflammatory response through inhibiting $NF-{\kappa}B$ following AMP kinase activation in RAW 264.7 macrophages. PLoS ONE 7: e53101. https://doi.org/10.1371/journal.pone.0053101