Determination of Glycyrrhizic Acid Content and Anti-Diabetic Effect of Glycyrrhiza uralensis Depending on Cultivation Region

재배지역별 감초의 Glycyrrhizic Acid 함량 분석과 항당뇨 효능 평가

  • Jang, Da Eun (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Song, Jin (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hwang, In Guk (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Sang Hoon (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choe, Jeong-Sook (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hwang, Kyung-A (Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration)
  • 장다은 (국립농업과학원 기능성식품과) ;
  • 송진 (국립농업과학원 기능성식품과) ;
  • 황인국 (국립농업과학원 기능성식품과) ;
  • 이상훈 (국립농업과학원 기능성식품과) ;
  • 최정숙 (국립농업과학원 기능성식품과) ;
  • 황경아 (국립농업과학원 기능성식품과)
  • Received : 2016.08.18
  • Accepted : 2016.09.27
  • Published : 2017.01.31


This study investigated the glycyrrhizic acid content and anti-diabetic activities of Glycyrrhiza uralensis (GU) depending on the cultivation region (Jecheon, Youngju, Gokseong, China, and Uzbekistan). Glycyrrhizic acid accuracy recovery and intra- and inter-day precisions (RSD%) of the method were calculated at 99.10~107.07% and 3.92 and 6.31% for GU samples, respectively, whereas the limits of detection and quantitation were 0.14 and $0.20{\mu}g/mL$. Anti-diabetic activity was measured by ${\alpha}-glucosidase$ and glucose uptake. GU (20 g) was extracted with 70% ethanol at $70^{\circ}C$ for 6 h. The Jecheon and Gokseong GU showed good inhibitory activity compared to the control. The Jecheon, Youngju, and Uzbekistan GU ethanol extracts ($100{\mu}g/mL$) showed glucose uptakes (in $C_2C_{12}$ myotube) of 124.19, 127.18, and 126.92%, respectively, compared to the positive control. In conclusion, these methods were validated for detection of glycyrrhizic acid in GU, and the results indicate that GU might have potential anti-diabetic activities.


Supported by : 국립농업과학원


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