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Microbial bioconversion of natural Philippine nut oils into a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid

미생물 생변환을 통한 필리핀 너트유로부터 기능성 지방산 7,10-dihydroxy-8(E)-octadecenoic acid 생산

  • Dasangrandhi, Chakradhar (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Ellamar, Joel B. (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Young Soon (Department of Food and Nutrition, Korea University) ;
  • Kim, In Hwan (Department of Food and Nutrition, Korea University) ;
  • Kim, Hak-Ryul (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2016.09.08
  • Accepted : 2016.10.31
  • Published : 2017.02.28

Abstract

Biocatalytic modification of natural resources can be used to generate novel compounds with specific properties, such as higher viscosity and reactivity. The production of hydroxy fatty acids (HFAs), originally found in low quantities in plants, is a good example of the biocatalytic modification of natural vegetable oils. HFAs show high potential for application in a wide range of industrial products, including resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. In a recent study, Pseudomonas aeruginosa strain PR3 was used to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid. This present study focused primarily on the utilization of three natural nut oils obtained from the Philippines -pili nut oil (PNO), palm oil (PO), and virgin coconut oil (VCO)- to produce DOD by P. aeruginosa strain PR3. Strain PR3 produced DOD from PNO and PO only, with PNO being the more efficient substrate. An optimization study to achieve the maximum DOD yield from PNO revealed the optimal incubation time and medium pH to be 48 h and 8.0, respectively. Among the carbon sources tested, fructose was the most efficiently used, with a maximum DOD production of 130 mg/50 mL culture. Urea was the optimal nitrogen source, with a maximum product yield of 165 mg/50 mL culture. The results from this study demonstrated that PNO could be used as an efficient substrate for DOD production by microbial bioconversion.

Keywords

bioconversion;hydroxy fatty acid;natural nut oil;Pseudomonas aeruginosa

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Chang IA, Kim IH, Kang SC, Hou CT, Kim HR. Production of 7,10-dihydroxy-8(E)-octadecenoic acid from triolein via lipase induction by Pseudomonas aeruginosa PR3. Appl. Microbiol. Biotechnol. 74: 301-306 (2007) https://doi.org/10.1007/s00253-006-0662-5
  2. Bae JH, Suh MJ, Lee NY, Hou CT, Kim HR. Production of a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid, from high oleic safflower oil by Pseudomonas aeruginosa PR3. Biotechnol. Bioproc. Eng. 15: 953-958 (2010) https://doi.org/10.1007/s12257-010-0057-4
  3. Hou CT, Bagby MO, Plattner RD, Koritala S. A novel compound, 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid by bioconversion. J. Am. Oil Chem. Soc. 68: 99-101 (1991) https://doi.org/10.1007/BF02662326
  4. Kim H, Gardner HW, Hou CT. 10(S)-hydroxy-8(E)-octadecenoic acid, an intermediate in the conversion of oleic acid to 7,10-dihydroxy- 8(E)-octadecenoic acid. J. Am. Oil Chem. Soc. 77: 95-99 (2000) https://doi.org/10.1007/s11746-000-0015-7
  5. Suh MJ, Baek KY, Kim BS, Hou CT, Kim HR. Production of 7,10-dihydroxy-8(E)-octadecenoic acid from olive oil by Pseudomonas aeruginosa PR3. Appl. Micrbiol. Biotechnol. 89: 1721-1727 (2011) https://doi.org/10.1007/s00253-010-3040-2
  6. Chang IA, Bae JH, Suh MJ, Kim IH, Hou CT, Kim HR. Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3. Appl. Microbiol. Biotechnol. 78: 581-586 (2008) https://doi.org/10.1007/s00253-007-1342-9
  7. Kim H, Jang YS, Hou CT. Effect of metal ions on the production of isomeric 9,10,13 (9,12,13)-trihydroxy-11E(10E)-octadecenoic acid from linoleic acid by Pseudomonas aeruginosa PR3. Enz. Microbial. Technol. 30: 752-757 (2002) https://doi.org/10.1016/S0141-0229(02)00053-4
  8. Kim H, Gardner HW, Hou CT. Production of isomeric (9,10,13)- trihydroxy-11E(10E)-octadecenoic acid from linoleic acid by Pseudomonas aeruginosa PR3. J. Ind. Microbiol. Biotechnol. 25: 109-115 (2000) https://doi.org/10.1038/sj.jim.7000041
  9. Kato T, Yamaguchi Y, Abe N, Uyehara T, Nakai T, Yamanaka S, Harada N. Unsaturated hydroxy fatty acids, the self-defensive substances in rice plant against rice blast disease. Chem. Lett. 25: 409-412 (1984)
  10. Hou CT, Forman RJ. Growth inhibition of plant pathogenic fungi by hydroxy fatty acids. J. Ind. Microbiol. Biotechnol. 24: 275-276 (2000) https://doi.org/10.1038/sj.jim.2900816
  11. Bajpai V, Shin SY, Kim MJ, Kim HR, Kang SC. Antifungal activity of bioconverted oil extract of linoleic acid and fractionated dilutions against phytopathogens Rhizoctonia solani and Botrytis cinerea. Agric. Chem. Biotechnol. 47: 199-204 (2004)
  12. Shin SY, Kim HR, Kang SC. Antibacterial activity of various hydroxy fatty acids bioconverted by Pseudomonas aeruginosa PR3. Agric. Chem. Biotechnol. 47: 205-208 (2004)
  13. Sohn HR, Bae JH, Hou CT, Kim HR. Antibacterial activity of a 7,10-dihydroxy-8(E)-octadecenoic acid against plant pathogenic bacteria. Enz. Microb. Technol. 53: 152-153 (2013) https://doi.org/10.1016/j.enzmictec.2013.02.009
  14. Sohn HR, Back KY, Hou CT, Kim HR. Antibacterial activity of a 7,10-dihydroxy-8(E)-octadecenoic acid against food-born pathogenic bacteria. Biocatal. Agr. Biotechnol. 2: 85-87 (2013)
  15. Kuo TM, Kim H, Hou CT. Production of a novel compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa PR3. Curr. Microbiol. 43: 198-203 (2001) https://doi.org/10.1007/s002840010287
  16. Ellamar JB, Song KS, Kim HR. One-step production of a biologically active novel furan fatty acid from 7,10-dihydroxy-8(E)-octadecenoic acid. J. Agr. Food Chem. 59:.8175-8179 (2011) https://doi.org/10.1021/jf2015683

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