Antioxidant activities of phenolic compounds from Angelica gigas Nakai extract using subcritical water

아임계수 추출 기술을 이용한 당귀 추출물의 유효성분 및 산화방지 평가

  • Ko, Min-Jung (Department of Food Science and Engineering, Ewha Womans University) ;
  • Lee, Jeong-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Nam, Hwa-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University)
  • 고민정 (이화여자대학교 식품공학과) ;
  • 이정현 (이화여자대학교 식품공학과) ;
  • 남화현 (이화여자대학교 식품공학과) ;
  • 정명수 (이화여자대학교 식품공학과)
  • Received : 2016.07.08
  • Accepted : 2016.09.02
  • Published : 2017.02.28


Subcritical water extraction can be used to selectively extraction compounds by varying the temperature-dependent dielectric constant of water. This study investigated subcritical water extraction of decursin and nodakenin yields from Angelica gigas Nakai (AN) quantitatively and qualitatively by HPLC, and HPLC-ESI/MS. Total phenolics, total flavonoid contents, and antioxidant activity were determined by DPPH and ABTS radical scavenging activity, including the effects of varying the extraction conditions of temperature ($110-200^{\circ}C$) and time (1-20 min) under high pressure (10 MPa). By subcritical water extraction under operating conditions of $120-130^{\circ}C$, the maximum yields of decursin ($6.64{\pm}0.42%$ in the dried material) and nodakenin ($3.71{\pm}0.28%$ in the dried material) were obtained. From $190-200^{\circ}C$ the maximum yields of total phenolics ($75.97{\pm}1.64mg$ gallic acid equivalent/g AN), flavonoids ($8.56{\pm}1.10mg$ quercetin equivalent/g AN), DPPH ($63.07{\pm}1.71%$), and ABTS ($72.32{\pm}2.82%$) were obtained.


subcritical water extraction;Angelica gigas Nakai;decursin;nodakenin;antioxidant activity


Supported by : 한국연구재단, 농림수산식품기술기획평가원


  1. Ko MJ, Cheigh CI, Cho SW, Chung MS. Subcritical water extraction of flavonol quercetin from onion skin. J. Food Eng. 102: 327-333 (2011)
  2. Ko MJ, Cheigh CI, Chung MS. Relationship analysis between flavonoids structure and subcritical water extraction (SWE). Food Chem. 143: 147-155 (2014)
  3. Ko MJ, Cheigh CI, Chung MS. Optimization of subcritical water extraction of flavanols from green tea leaves. J. Agr. Food Chem. 65: 6828-6833 (2014)
  4. Ko MJ, Kwon HL, Chung MS. Optimum conditions for extracting flavanones from grapefruit peels and encapsulation of extracts. Korean J. Food Sci. Technol. 46: 465-469 (2014)
  5. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agr. Food Chem. 51: 2144-2155 (2003)
  6. Hwang JB, Yang MO. Comparison of chemical components of Angelica gigas Nakai and Angelica acutiloba Kitagawa. Korean J. Food Sci. Technol. 29: 1113-1118 (1997)
  7. Um YR, Lee JH, Ma JY. Quantitative analysis of marker substances in solid fermented Angelicae gigantis radix by HPLC. Korean J. Oriental Med. 16: 173-178 (2010)
  8. Park KW, Choi SR, Shon MY, Jeong IY, Kang KS, Lee ST, Shim KH, Seo KI. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J Korean Soc. Food Sci. Nutr. 36: 1385-1390 (2007)
  9. Cha, JY, Kim HW, Heo JS, Ahn HY, Eom KE, Heo SJ, Cho YS. Ingredients analysis and biological activity of fermented Angelica gigas Nakai by mold. J. Life Sci. 20: 1385-1393 (2010)
  10. Heo JS, Cha JY, Kim HW, Ahn HY, Eom KE, Heo SJ, Cho YS. Bioactive materials and biological activity in the extracts of leaf, stem mixture and root from Angelica gigas Nakai. J. Life Sci. 20: 750-759 (2010)
  11. Kang SA, Han JA, Jang KH, Choue RW. DPPH radical scavenger activity and antioxidant effects of chan-dang-gui (Angelica gigas). J. Korean Soc. Food Sci. Nutr. 33: 1112-1118 (2004)
  12. Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem. 112: 303-309 (2009)
  13. Cicco N, Lanorte MT, Paraggio M, Viggiano M, Lattanzio V. A reproducible, rapid and inexpensive Folin-Ciocalteu micromethod in determining phenolics of plant methanol extracts. Microchem. J. 91: 107-110 (2009)
  14. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999)
  15. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958)
  16. Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic cotribution to total antioxidant activity. Food Chem. 73: 239-244 (2001)
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999)
  18. Ahn MJ, Lee MK, Kim YC, Sung SH. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J. Pharmaceut. Biomed. 46: 258-266 (2008)
  19. Park JH, Jung JW, Kweon KT, Seo MJ, Seo EK, Park YK, Lee JH. Nodakenin and decursin contents of fermented Angelicae gigantis radix by 4 species strain. Korean J. Herbol. 25: 7-10 (2010)
  20. Li HB, Wong CC, Cheong KW, Chen F. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Sci. Technol. 41: 385-390 (2008)
  21. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Food Drug Anal. 22: 296-302 (2014)