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Antioxidant activities of phenolic compounds from Angelica gigas Nakai extract using subcritical water

아임계수 추출 기술을 이용한 당귀 추출물의 유효성분 및 산화방지 평가

  • Ko, Min-Jung (Department of Food Science and Engineering, Ewha Womans University) ;
  • Lee, Jeong-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Nam, Hwa-Hyun (Department of Food Science and Engineering, Ewha Womans University) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University)
  • 고민정 (이화여자대학교 식품공학과) ;
  • 이정현 (이화여자대학교 식품공학과) ;
  • 남화현 (이화여자대학교 식품공학과) ;
  • 정명수 (이화여자대학교 식품공학과)
  • Received : 2016.07.08
  • Accepted : 2016.09.02
  • Published : 2017.02.28

Abstract

Subcritical water extraction can be used to selectively extraction compounds by varying the temperature-dependent dielectric constant of water. This study investigated subcritical water extraction of decursin and nodakenin yields from Angelica gigas Nakai (AN) quantitatively and qualitatively by HPLC, and HPLC-ESI/MS. Total phenolics, total flavonoid contents, and antioxidant activity were determined by DPPH and ABTS radical scavenging activity, including the effects of varying the extraction conditions of temperature ($110-200^{\circ}C$) and time (1-20 min) under high pressure (10 MPa). By subcritical water extraction under operating conditions of $120-130^{\circ}C$, the maximum yields of decursin ($6.64{\pm}0.42%$ in the dried material) and nodakenin ($3.71{\pm}0.28%$ in the dried material) were obtained. From $190-200^{\circ}C$ the maximum yields of total phenolics ($75.97{\pm}1.64mg$ gallic acid equivalent/g AN), flavonoids ($8.56{\pm}1.10mg$ quercetin equivalent/g AN), DPPH ($63.07{\pm}1.71%$), and ABTS ($72.32{\pm}2.82%$) were obtained.

Keywords

subcritical water extraction;Angelica gigas Nakai;decursin;nodakenin;antioxidant activity

Acknowledgement

Supported by : 한국연구재단, 농림수산식품기술기획평가원

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