Effects of Pleurotus eryngii extract against inflammation in activated RAW 264.7 cells and UV-induced skin damage in mice

활성 RAW 264.7 세포에서 항염증 및 자외선 유도 마우스 피부손상의 개선에 대한 새송이 추출물의 효과

  • 조병옥 ((주)아토큐앤에이 기업부설연구소) ;
  • 윤홍화 ((주)아토큐앤에이 기업부설연구소) ;
  • 이현서 (전주대학교 보건관리학과) ;
  • 추정임 (전주대학교 농생명치유산업연구소) ;
  • 장선일 ((주)아토큐앤에이 기업부설연구소)
  • Received : 2016.08.22
  • Accepted : 2016.10.07
  • Published : 2017.02.28


The aim of this study was to investigate the anti-inflammatory and ultraviolet (UV)-protective effect of Pleurotus eryngii extract (PEE) in activated RAW 264.7 cells and UV-induced mouse skin damage. The results showed that PEE strongly inhibited the production of inflammatory mediators such as nitric oxide, interleukin $(IL)-1{\beta}$, and IL-6 at high concentrations in LPS-stimulated RAW 264.7 macrophages. In addition, PEE treatment suppressed erythema, melanin index, and epidermal thickness to a greater degree than ascorbic acid (AA) treatment in UV-irradiated mice. Finally, PEE treatment inhibited the infiltration of mast cell to a similar degree of AA treatment. Therefore, these results indicate that PEE could improve inflammation and skin damage in immune cells and UV-irradiated mice. This study may provide positive insights into PEE as a functional food and cosmetic ingredient for treatment of inflammation and skin damage.


Supported by : 한국산업기술진흥원


  1. Melnikova VO, Ananthaswamy HN. Cellular and molecular events leading to the development of skin cancer. Mutat. Res. 571: 91-106 (2005)
  2. Clydesdale GJ, Dandie GW, Muller HK. Ultraviolet light induced injury: Immunological and inflammatory effects. Immunol. Cell Biol. 79: 547-568 (2001)
  3. Chung JH, Seo JY, Lee MK, Eun HC, Lee JH, Kang S, Fisher GJ, Voorhees JJ. Ultravioletmodulation of human macrophage metalloelastase in human skin in vivo. J. Invest. Dermatol. 119: 507-512 (2002)
  4. Weichenthal M, Godorr M, Altenhoff J, Neuber K, Breitbart EW. Effects of whole-body UVB irradiation on cytokine production by peripheral blood mononuclear cells from stage I melanoma patients. Arch. Dermatol. Res. 292: 348-353 (2000)
  5. Lopez-Camarillo C, Ocampo EA, Casamichana ML, Perez-Plasencia C, Alvarez-Sanchez E, Marchat LA. Protein kinases and transcription factors activation in response to UV-radiation of skin: Implications for carcinogenesis. Int. J. Mol. Sci. 13: 142-172 (2012)
  6. Li WH, Pappas A, Zhang L, Ruvolo E, Cavender D. IL-11, IL- 1${\alpha}$, IL-6, and TNF-${\alpha}$ are induced by solar radiation in vitro and may be involved in facial subcutaneous fat loss in vivo. J. Dermatol. Sci. 71: 58-66 (2013)
  7. Hiromi N, Nakano H, Matsuzaki Y, Sawamura D, Hanada K. Immunohistochemical analysis of in vivo UVB-induced secretion of IL-1${\alpha}$ and IL-6 in keratinocytes. Mol. Med. Rep. 4: 611-614 (2011)
  8. Bhm M, Luger TA, Tobin DJ, Garca-Borrn JC. Melanocortin receptor ligands: New horizons for skin biology and clinical dermatology. J. Invest. Dermatol. 126: 1966-1975 (2006)
  9. Zhang X, Xie YL, Yu XT, Su ZQ, Yuan J, Li YC, Su ZR, Zhan JY, Lai XP. Protective effect of super-critical carbon dioxide fluid extract from flowers and buds of Chrysanthemum indicum Linnn against ultraviolet-induced photo-aging in mice. Rejuvenation Res. 18: 437-448 (2015)
  10. Divya SP, Wang X, Pratheeshkumar P, Son YO, Roy RV, Kim D, Dai J, Hitron JA, Wang L, Asha P, Shi X, Zhang Z. Blackberry extract inhibits UVB-induced oxidative damage and inflammation through MAP kinases and NF-${\kappa}B$ signaling pathways in SKH-1 mice skin. Toxicol. Appl. Pharmacol. 284: 92-99 (2015)
  11. Kawai J, Andoh T, Ouchi K, Inatomi S. Pleurotus eryngii ameliorates lipopolysaccharide-induced lung inflammation in mice. Evid. Based Complement. Alternat. Med. 2014: 532389 (2014)
  12. Choi JH, Kim HG, Jin SW, Han EH, Khanal T, Do MT, Hwang YP, Choi JM, Chun SS, Chung YC, Jeong TC, Jeong HG. Topical application of Pleurotus eryngii extracts inhibits 2,4-dinitrochlorobenzene- induced atopic dermatitis in NC/Nga mice by the regulation of Th1/Th2 balance. Food Chem. Toxicol. 53: 38-45 (2013)
  13. Nasti TH, Timares L. Inflammasome activation of IL-1 family mediators in response to cutaneous photodamage. Photochem. Photobiol. 88: 1111-1125 (2012)
  14. Xiang F, Lucas R, de Gruijl F, Norval M. A systematic review of the influence of skin pigmentation on changes in the concentrations of vitamin D and 25-hydroxyvitamin D in plasma/serum following experimental UV irradiation. Photochem. Photobiol. Sci. 14: 2138-2146 (2015)
  15. El-Abaseri TB, Hammiller B, Repertinger SK, Hansen LA. The epidermal growth factor receptor increases cytokine production and cutaneous inflammation in response to ultraviolet irradiation. ISRN Dermatol. 2013: 848705 (2013)
  16. Kwon HJ. Anti-melanogenesis activity of Pleurotus eringii extracts. Kor. J. Aesthet. Cosmetol. 13: 695-700 (2015)
  17. Song JH, Bae EY, Choi G, Hyun JW, Lee MY, Lee HW, Chae S. Protective effect of mango (Mangifera indica L.) against UVBinduced skin aging in hairless mice. Photodermatol. Photoimmunol. Photomed. 29: 84-89 (2013)
  18. Rodriguez Estrada AE, Royse DJ. Yield, size and bacterial blotch resistance of Pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese, copper and whole ground soybean. Bioresour. Technol. 98: 1898-1906 (2007)
  19. Mattila P, KnkK, Eurola M, Pihlava JM, Astola J, Vahteristo L, Hietaniemi V, Kumpulainen J, Valtonen M, Piironen V. Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. J. Agr. Food Chem. 49: 2343-2348 (2001)
  20. Kalyoncu F, Oskay M, Saglam H, Erdogan TF, Tamer AU. Antimicrobial and antioxidant activities of mycelia of 10 wild mushroom species. J. Med. Food. 13: 415-419 (2010)
  21. Han EH, Hwang YP, Kim HG, Choi JH, Im JH, Yang JH, Lee HU, Chun SS, Chung YC, Jeong HG. Inhibitory effect of Pleurotus eryngii extracts on the activities of allergic mediators in antigen- stimulated mast cells. Food Chem. Toxicol. 49: 1416-1425 (2011)
  22. Choi JH, Kim HG, Jin SW, Han EH, Khanal T, Do MT, Hwang YP, Choi JM, Chun SS, Chung YC, Jeong TC, Jeong HG. Topical application of Pleurotus eryngii extracts inhibits 2,4-dinitrochlorobenzene- induced atopic dermatitis in NC/Nga mice by the regulation of Th1/Th2 balance. Food Chem. Toxicol. 53: 38-45 (2013)
  23. Ike K, Kameyama N, Ito A, Imai S. Induction of a T-Helper 1 (Th1) immune response in mice by an extract from the Pleurotus eryngii (Eringi) mushroom. J. Med. Food. 15: 1124-1128 (2012)
  24. Yin HH, Cho BO, Lee HS, Chu JL, Jang SI. Synergistic Effects of grape branch and Pleurotus eryngii extract combination against Inflammation in activated mast cells and atopic dermatitis-like skin lesion in mice. Korean J. Food Sci. Biotechnol. in press (2016)
  25. Lin JT, Liu CW, Chen YC, Hu CC, Juang LD, Shiesh CC, Yang. DJ. Chemical composition, antioxidant and anti-inflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time. LWT-Food Sci. Technol. 55: 374-382 (2014)
  26. Kim HK, Han HS, Lee GD, Kim KH. Physiological activities of Pleurotus eryngii extracts. J. Korean Soc. Food. Sci. Nutr. 34: 439-445 (2005)
  27. Lee C, Park GH, Ahn EM, Kim BA, Park CI, Jang JH. Protective effect of codium fragile against UVB-induced pro-inflammatory and oxidative damages in HaCaT cells and BALB/c mice. Fitoterapia 86: 54-63 (2013)
  28. Rossol M, Heine H, Meusch U, Quandt D, Klein C, Sweet MJ, Hauschildt S. LPS-induced cytokine production in human monocytes and macrophages. Crit. Rev. Immunol. 31: 379-446 (2011)
  29. Pacholczyk M, Czernicki J, Ferenc T. The effect of solar ultraviolet radiation (UVR) on induction of skin cancers. Med. Pr. 67: 255-266 (2016)
  30. Hiramoto K, Kobayashi H, Yamate Y, Ishii M, Sato EF. Intercellular pathway through hyaluronic acid in UVB-induced inflammation. Exp. Dermatol. 21: 911-914 (2012)
  31. Strickland I, Rhodes LE, Flanagan BF, Friedmann PS. TNF-alpha and IL-8 are upregulated in the epidermis of normal human skin after UVB exposure: Correlation with neutrophil accumulation and E-selectin expression. J. Invest. Dermatol. 108: 763-768 (1997)