DOI QR코드

DOI QR Code

Effects of Bia-hwan (Féiér-wán) on the Ovariectomized Rat Model of Osteoporosis

비아환(肥兒丸)이 난소적출로 유발된 흰쥐의 골다공증 모델에 미치는 영향

  • Received : 2017.03.23
  • Accepted : 2017.04.10
  • Published : 2017.04.30

Abstract

Objectives Osteoporosis is a common disease in adults with prevalence rate of more than 40%, and occurs more to female than to male. The purpose of this study is to identify the effect of Bia-hwan ($F{\acute{e}}i{\acute{e}}r$-$w{\acute{a}}n$) on the osteoporosis of ovariectomized rats. Methods 24 female rats were randomly assigned to a SHAM group, a control group, and a BAH group. Ovaries of the control group and the BAH group were extracted. After than, general animal feed were given to the SHAM group and the control group, animal feed contained BAH were given to the BAH group through mouths of them. After 8 weeks, rats were sacrificed and their weight, calcium, phosphorus, estradiol, total-cholesterol, triglyceride, ALP, albumin, AST, ALT, the weight of femur and tibia ash per body ratio, both area and thickness of trabecular bone, the area of osteoblast and the number of osteoclast were measured. Results The level of calcium, phosphorus, AST in the serum of the BAH group increased significantly compared to the control group. There was no significant difference between the control group and the BAH group in the level of estradiol, total-cholesterol, triglyceride, ALP, albumin, and ALT in the serum. There was no significant difference between the control group and the BAH group in the weight of femur and tibia ash per body ratio. The thickness of trabecular of the BAH group increased significantly compared to the control group, but there was no significant difference in the trabecular area. The area of osteoblast and the number of osteoclast of the BAH group decreased significantly compared to the control group. Conclusions From the results of the above study, oral intake of BAH can inhibit the decrease of calcium, phosphorus in blood and prevent the thinning process of the trabecular by suppressing activation of osteoclast. Thus, BAH may have effects on the treatment and prevention of osteoporosis.

Keywords

Bia-hwan ($F{\acute{e}}i{\acute{e}}r$-$w{\acute{a}}n$);Ovariectomy;Osteoporosis;Osteoclast;Trabecular thickness

References

  1. Essentials of nutrition. Kyomunsa:Paju. 2011:280.
  2. The Society of Korean Medicine Rehabilitation. Korea Rehabilitation Medicine. Seoul:Koonja. 2012:9-10, 165-9, 350-63.
  3. Beatrice C. Lupsa, Karl Insogna. Bone Health and Osteoporosis. Endocrinology and Metabolism Clinics of North America. 2016;44(3):517-30.
  4. Kim JG, Moon YW. Diagnosis of Osteoporosis. Hip and Pelvis. 2011;23(2):108-15.
  5. Yoon HS. Factors Associated with Decreased Bone Mineral Density in Korean Adults. Chosun University. 2011.
  6. 今釋黃帝內經 素問. 裵秉哲. Seoul:Sungbosa. 1994:292-5, 383-7.
  7. Oriental medical Prescription. Seoul:Younglim publishing company. 2012:576.
  8. 東醫寶鑑編修刊行委員會. 東醫寶鑑. Seoul:民衆書閣. 1993:649-53.
  9. Ko SC, Won JH, Jung KM. Studies on the Anti-allergic Effect of Biahwan and Sutodan in Rats and Mice. The Journal of Korean Oriental Pediatrics. 1991;5(1):1-14.
  10. Lee HY, Park SH, Chae SW, Soung NK, Oh MJ, Kim JS, Kim YO, Chae HJ. Aqueous ginseng extract has a preventive role in RANKL-induced osteoclast differentiation and estrogen deficiency-induced osteoporosis. Journal of functional foods. 2015;13:192-203. https://doi.org/10.1016/j.jff.2014.12.039
  11. Park ST, Lee MS, Jeon BH, Park KI, Oh JM. Effect of Atractylodis Rhizoma Alba on Osteoclast Formation. The Korean Journal of Oriental Physiology & Pathology. 2011.2;25(1):109-14.
  12. Cheon YH, Kwack SC, Oh JM, Choi MK, Kim JJ, Kwak HB, Lee MS, Jeon BH, Moon SY. Effect of Hoelen in RANKL-induced Osteoclast Differentiation. The Korean Journal of Oriental Physiology & Pathology. 2012.6;26(3):320-4.
  13. Mathavan N1, Turunen MJ, Tagil M, Isaksson H. Characterising bone material composition and structure in the ovariectomized (OVX) rat model of osteoporosis. Calcif Tissue Int. 2015 Aug;97(2):134-44. https://doi.org/10.1007/s00223-015-9991-7
  14. Choi JK, Yoo DY. The Effect of Kamijoaguiem on Osteoporosis Induced by Ovariectomy in Rats. The Journal of Oriental Obstetrics and Gynecology. 2006;19(2):107-26.
  15. Hong-sik Choi. The Efficacy of Cynomorii Herba and Eucommiae Cortex on Treatment of Osteoporosis in Ovariectomized Rats. Kor. J. Herbology 2008;23(2):19-24.
  16. Sun-Hye Lim, Tae-Youl Ha, Sung-Ran Kim, Jiyun Ahn, Hyun Jin Park and Suna Kim. Ethanol extract of Psoralea corylifolia L. and its main constituent, bakuchiol, reduce bone loss in ovariectomised Sprague-Dawley rats. British Journal of Nutrition 2009;101:1031-9. https://doi.org/10.1017/S0007114508066750
  17. Choi HS. The Efficacy of Cynomorii Herba and Eucommiae Cortex on Treatment of Osteoporosis in Ovariectomized Rats. The Korean Journal of Herbology. 2008;23(2):19-24.
  18. Tianxiu Wu, Lin Zou, Honghua He, Kefeng Wu. Prevention of Radix Rehmanniae Preparata on Glucocorticoid-Induced Osteoporosis in Rats. Chemical engineering transactions. 2015;46:67-72.
  19. 脾系內科學. 柳基遠. Seoul:Dongwoo publishing company. 2000:3-11.
  20. Korean Society for Laboratory Medicine. Laboratory Medicine. Seoul:E-public. 2009:291-8, 307-14, 337-44, 374, 385-91.
  21. Silver DS. Calcium and vitamin D controversies. Rheum Dis Clin North Am. 2011 Aug;37(3):351-63. https://doi.org/10.1016/j.rdc.2011.07.005
  22. Park Y, Moon HJ, Paik DJ, Kim DY. Effect of dietary legumes on bone-specific gene expression in ovariectomized rats. Nutr Res Pract. 2013 Jun;7(3):185-91. https://doi.org/10.4162/nrp.2013.7.3.185
  23. Wei J, Wang J, Gong Y, Zeng R. Effectiveness of combined salmon calcitonin and aspirin therapy for osteoporosis in ovariectomized rats. Mol Med Rep. 2015 Aug;12(2):1717-26. https://doi.org/10.3892/mmr.2015.3637
  24. Pickar JH, Bon C, Amadio JM, Mirkin S, Bernick B. Pharmacokinetics of the first combination $17{\beta}$-estradiol/progesterone capsule in clinical development for menopausal hormone therapy. Menopause. 2015 Dec;22(12):1308-16. https://doi.org/10.1097/GME.0000000000000467
  25. Carroll M, Kit B, Lacher D. Trends in elevated triglyceride in adults: United States, 2001-2012. NCHS Data Brief. 2015 May;(198):198.
  26. Hlaing TT, Compston JE. Biochemical markers of bone turnover - uses and limitations. Ann Clin Biochem. 2014 Mar;51(Pt 2):189-202. https://doi.org/10.1177/0004563213515190
  27. Chiang SS, Chang SP, Pan TM. Osteoprotective effect of Monascus-fermented dioscorea in ovariectomized rat model of postmenopausal osteoporosis. J Agric Food Chem. 2011 Sep 14;59(17):9150-7. https://doi.org/10.1021/jf201640j
  28. Goorden SM, Buffart TE, Bakker A, Buijs MM. [Liver disorders in adults: ALT and AST]. Ned Tijdschr Geneeskd. 2013;157(43):A6443.