DOI QR코드

DOI QR Code

Protective effects of red ginseng treated with gold nanoparticles against H2O2-induced oxidative stress in neuronal PC-12 cells

금 나노입자를 처리한 홍삼의 산화스트레스 완화 및 PC-12 신경세포 보호

  • Received : 2016.11.11
  • Accepted : 2017.02.03
  • Published : 2017.04.30

Abstract

Red ginseng prepared from fresh 6-year-old ginseng treated with colloidal gold nanoparticles was extracted using hot water to evaluate its total phenolic and flavonoid contents, antioxidant capacity, and neuroprotective effects. Water extract of red ginseng treated with gold nanoparticles (WERGGN) had total phenolic and total flavonoid contents of 212.2 mg gallic acid equivalents/$^{\circ}Bx$ and 3.5 mg catechin equivalents/$^{\circ}Bx$, respectively. The antioxidant capacities of WERGGN measured using ABTS, DPPH, and ORAC assays were 272.3, 141.2, and 868.4 mg vitamin C equivalents/$^{\circ}Bx$, respectively. The WERGGN showed protective effects on the viability of neuron-like PC-12 cells against oxidative stress induced by hydrogen peroxide in a dose-dependent manner, partly because of a reduction in intracellular oxidative stress. Acetylcholinesterase and butyrylcholinesterase, which degrade the neurotransmitter acetylcholine to terminate neurotransmission, were inhibited by treatment with WERGGN. These results suggest that WERGGN is useful as a functional material to decrease oxidative stress and neuronal damage.

Keywords

acetylcholinesterase;antioxidant capacity;butyrylcholinesterase;colloidal gold nanoparticles;red ginseng

References

  1. Prince M, Bryce R, Albanese E, Wimo A, Ribeiro W, Ferri CP. The global prevalence of dementia: A systematic review and metaanalysis. Alz. Dimentia 9: 63-75 (2013) https://doi.org/10.1016/j.jalz.2012.11.007
  2. Mount C, Downton C. Alzheimer disease: Progress or profit? Nat. Med. 12: 780-784 (2006) https://doi.org/10.1038/nm0706-780
  3. Szwajgier D. Acetylcholinesterase activity of phenolic acids and their derivatives. Z. Naturforsch. 68c: 125-132 (2013)
  4. Lee SM, Bae BS, Park HW, Ahn NG, Cho BG, Cho YL, Kwak YS. Characterization of Korean red ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition. J. Ginseng Res. 39: 384-391 (2015) https://doi.org/10.1016/j.jgr.2015.04.009
  5. Hwang CR, Lee SH, Jang GY, Hwang IG, Kim HY, Woo KS, Lee J, Jeong HS. Changes in ginsenoside compositions and antioxidant activities of hydroponic-cultured ginseng roots and leaves with heating temperature. J. Ginseng Res. 38: 180-186 (2014) https://doi.org/10.1016/j.jgr.2014.02.002
  6. Kim YC, Hong HD, Rho J, Cho CW, Rhee YK, Yim JH. Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times. J. Ginseng Res. 31: 230-236 (2007) https://doi.org/10.5142/JGR.2007.31.4.230
  7. Chung IM, Lim JJ, Ahn MS, Jeong HN, An TJ, Kim SH. Comparative phenolic compound profiles and antioxidative activity of the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) according to cultivation years. J. Ginseng Res. 40: 68-75 (2016) https://doi.org/10.1016/j.jgr.2015.05.006
  8. Lee CH, Kim JH. A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases. J. Ginseng Res. 38: 161-166 (2014) https://doi.org/10.1016/j.jgr.2014.03.001
  9. Kang H, Hwang YG, Lee TG, Jin CR, Cho CH, Jeong HY, Kim D-O. Use of gold nanoparticle fertilizer enhances the ginsenoside contents and anti-inflammatory effects of red ginseng. J. Microbiol. Biotechnol. 26: 1668-1674 (2016) https://doi.org/10.4014/jmb.1604.04034
  10. Liao B, Newmark H, Zhou R. Neuroprotective effects of ginseng total saponin and ginsenosides Rb1 and Rg1 on spinal cord neurons in vitro. Exp. Neurol. 173: 224-234 (2002) https://doi.org/10.1006/exnr.2001.7841
  11. Saxena M, Saxena J, Pradhan A. Flavonoids and phenolic acids as antioxidants in plants and human health. Int. J. Pharm. Sci. Rev. Res. 16: 130-134 (2012)
  12. Kim JY, Kim OY, Lee JH. The effect of Korean Panax red ginseng supplementation on antioxidant enzymes activities and lipid peroxides in Korean men. Korean J. Lipidol. 21: 94-103 (2011)
  13. Mook-Jung IH, Hong H-S, Boo JH, Lee KH, Yun SH, Cheong MY, Joo I, Huh K, Jung MW. Ginsenoside Rb1 and Rg1 improve spatial learning and increase hippocampal synaptophysin level in mice. J. Neurosci. Res. 63: 509-515 (2001) https://doi.org/10.1002/jnr.1045
  14. Mitra A, Chakraborty S, Auddy B, Tripathi P, Sen S, Saha A, Mukherjee B. Evaluation of chemical constituents and free-radical scavenging activity of Swarnabhasma (gold ash), an ayurvedic drug. J. Ethnopharmacol. 80: 147-153 (2002) https://doi.org/10.1016/S0378-8741(02)00008-9
  15. Jo MR, Bae SH, Go MR, Kim HJ, Hwang YG, Choi SJ. Toxicity and biokinetics of colloidal gold nanoparticles. Nanomaterials 5: 835-850 (2015) https://doi.org/10.3390/nano5020835
  16. Zhang XD, Wu HY, Wu D, Wang YY, Chang JH, Zhai ZB, Meng AM, Liu PX, Zhang LA, Fan FY. Toxicologic effects of gold nanoparticles in vivo by different administration routes. Int. J. Nanomed. 5: 771-787 (2010)
  17. Girgis E, Khalil WKB, Emam AN, Mohamed MB, Rao KV. Nanotoxicity of gold and gold-cobalt nanoalloy. Chem. Res. Toxicol. 25: 1086-1098 (2012) https://doi.org/10.1021/tx300053h
  18. Watanabe A, Kajita M, Kim J, Kanayama A, Takahashi K, Mashino T, Miyamoto Y. In vitro free radical scavenging activity of platinum nanoparticles. Nanotechnology 20: 455105 (2009) https://doi.org/10.1088/0957-4484/20/45/455105
  19. Kim YJ, Kim DB, Lee SY, Choi SY, Park JS, Lee SY, Park JW, Kwon HJ. Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells. BMB Rep. 42: 304-309 (2009) https://doi.org/10.5483/BMBRep.2009.42.5.304
  20. Hwang JS, Im S, Lee I, Kim TR, Kim D-O. Effects of Opuntia ficus-indica var. saboten ripe fruits on protection of neuronal PC-12 cells and cholinesterase inhibition. Korean J. Food Sci. Technol. 48: 86-91 (2016) https://doi.org/10.9721/KJFST.2016.48.1.86
  21. Kim D-O, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81: 321-326 (2003) https://doi.org/10.1016/S0308-8146(02)00423-5
  22. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 25-30 (1995) https://doi.org/10.1016/S0023-6438(95)80008-5
  23. Kim D-O, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agr. Food Chem. 50: 3713-3717 (2002) https://doi.org/10.1021/jf020071c
  24. Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agr. Food Chem. 50: 4437-4444 (2002) https://doi.org/10.1021/jf0201529
  25. Heo H-J, Cho H-Y, Hong B, Kim H-K, Kim E-K, Kim B-G, Shin D-H. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against $A{\beta}$-induced oxidative stress in PC12 cells. Amyloid-J. Protein Fold. Disord. 8: 194-201 (2001) https://doi.org/10.3109/13506120109007362
  26. Lee D, Ghafoor K, Moon S, Kim SH, Kim S, Chun H, Park J. Phenolic compounds and antioxidant properties of high hydrostatic pressure and conventionally treated ginseng (Panax ginseng) products. Qual. Assur. Saf. Crop. Food. 7: 493-500 (2015) https://doi.org/10.3920/QAS2014.0416
  27. Kim J-S. Investigation of phenolic, flavonoid, and vitamin contents in different parts of Korean ginseng (Panax ginseng C.A. Meyer). Prev. Nutr. Food Sci. 21: 263-270 (2016) https://doi.org/10.3746/pnf.2016.21.3.263
  28. Jin Y, Kim Y-J, Jeon J-N, Wang C, Min J-W, Jung S-Y, Yang DC. Changes of ginsenosides and physiochemical properties in ginseng by new 9 repetitive steaming and drying process. Korean J. Plant Res. 25: 473-481 (2012) https://doi.org/10.7732/kjpr.2012.25.4.473
  29. Lee JW, Mo EJ, Choi JE, Jo YH, Jang H, Jeong JY, Jin Q, Chung HN, Hwang BY, Lee MK. Effect of Korean red ginseng extraction conditions on antioxidant activity, extraction yield, and ginsenoside Rg1 and phenolic content: Optimization using response surface methodology. J. Ginseng Res. 40: 229-236 (2016) https://doi.org/10.1016/j.jgr.2015.08.001
  30. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 521-525 (2006)
  31. Kim D-O, Lee CY. Comprehensive study of vitamin C equivalent antioxidant capacity (VCEAC) of various polyphenolics in scavenging a free radical and its structural relationship. Crit. Rev. Food Sci. Nutr. 44: 253-273 (2004) https://doi.org/10.1080/10408690490464960
  32. Spencer JPE. The impact of fruit flavonoids on memory and cognition. Br. J. Nutr. 104: S40-S47 (2010) https://doi.org/10.1017/S0007114510003934
  33. Im SE, Yoon H, Nam TG, Heo HJ, Lee CY, Kim DO. Antineurodegenerative effect of phenolic extracts and caffeic acid derivatives in romaine lettuce on neuron-like PC-12 cells. J. Med. Food 13: 779-784 (2010) https://doi.org/10.1089/jmf.2009.1204
  34. Greig NH, Lahiri DK, Sambamurti K. Butyrylcholinesterase: An important new target in Alzheimer's disease therapy. Int. Psychogeriatr. 14 (Suppl 1): 77-91 (2002) https://doi.org/10.1017/S1041610203008676

Acknowledgement

Grant : 특구육성사업(연구소기업 전략육성사업)

Supported by : 연구개발특구진흥재단