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Studies on Anti-Inflammatory and Anti-Melanogenic Effect of Grape Fruit Stem Extract

포도송이가지 추출물의 항염증 및 미백효능에 대한 연구

  • 최안나 (전주대학교 의과학대학) ;
  • 이현서 (전주대학교 의과학대학) ;
  • 김장호 (전주대학교 의과학대학) ;
  • 조병옥 (전주대학교 의과학대학) ;
  • 신재영 (전주대학교 의과학대학) ;
  • 정승일 ((재)전주농생명소재연구원) ;
  • 장선일 (전주대학교 의과학대학)
  • Received : 2017.03.22
  • Accepted : 2017.05.20
  • Published : 2017.05.30

Abstract

Objectives : The various grape extracts derived from grape pulp, seed and skin, containing various types of polyphenols and flavonoids, have been known to have anti-inflammatory, antioxidant and improve cardiovascular condition as well as sun's damaging effects. However, there have been rare reports of various beneficial effects of grape fruit stem extract (GFSE), one of the waste products of grapes. We investigated anti-inflammatory and melanogenesis inhibitory effects of GFSE. Methods : One-hundred gram of grape fruit stem was extracted with 80% ethanol at room temperature for 3 days. After filtration, the ethanol was removed using vacuum evaporator, then lyophilized to obtain the dry extract which was stored at $-20^{\circ}C$ until used. NO levels were measured by using Greiss reagent. Prostaglandin $E_2$ ($PGE_2$) production was measured by ELISA assay. The expression levels of iNOS, COX-2, TRP-1 and TRP-2 were evaluated by western blot analysis. Results : GFSE reduced the level of nitric oxide and prostaglandin $E_2$ ($PGE_2$) production in a dose-dependent manner, compared to control. Expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein were also effectively inhibited by the GFSE. In a tyrosinase inhibitory activity, GFSE significantly reduced the tyrosinase activity and melanin content in a dose dependent manner, compared to control. GFSE also decreased the expression of tyrosinase related protein-1 (TRP-1) and tyrosinase related protein-2 (TRP-2), known as a melanocyte-specific gene product involved in melanin synthesis. Conclusions : Therefore, these results indicated that GFSE had powerful anti-inflammatory and anti-melanogenic effects.

Keywords

grape fruit stem;antioxidant;anti-inflammation;melanogenesis inhibition

Acknowledgement

Grant : 고욤나무와 울금 유래 바이오 활성물질을 활용한 코스메슈티컬 제품개발, 닥나무 소재의 생리활성 물질을 활용한 코스메슈티컬 시트(부직포), 액상소재 및 제품개발, 공간조성

Supported by : 중소기업청

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