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Antibacterial Activity of Graviola extract to inhibit the Staphylococcus epidermidis

Staphylococcus epidermidis를 억제하는 Graviola 추출물의 항균활성

  • Choi, Jong-Hwa (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University) ;
  • Ohk, Seung-Ho (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University)
  • 최종화 (전남대학교 대학원 향장품학 협동과정) ;
  • 옥승호 (전남대학교 대학원 향장품학 협동과정)
  • Received : 2017.03.16
  • Accepted : 2017.05.12
  • Published : 2017.05.31

Abstract

This study was carried out to verify the antimicrobial effect of Graviola leaves against pathogenic bacteria for the purpose of developing an antibacterial material. The extraction conditions of graviola leaves were $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$, and graviloa leaves werewater extracted at $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$.The extraction yields and extraction conditions were determined. The antimicrobial activity against S. epidermidis, S. aureus and E. coli TOP10 was evaluated by agar diffusion method. The extraction yields were 3.02%, 14.73% and 20.76% at $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$, respectively. The antimicrobial activity against S. epidermidis, S. aureus and E. coli TOP10 was found in S. epidermidis.In the samples extracted at $98^{\circ}C$, a clear zone of 13 mm was observed at 200 mg/mL and of 20 mm at 500 mg/mL. The MIC was 100 mg/mL. The higher the extraction temperature and concentration, the better was the growth inhibition effect. As a result, the natural antimicrobial activity contained in natural materials can solve the problem of resistance to antibiotics. It is considered that antimicrobial activity against S. epidermidis in skin is highly applicable to basic cosmetics and cosmetic materials.

Acknowledgement

Supported by : 한국연구재단

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