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Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis

젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과

  • Kim, Danil (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Eun-Kyung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Seong, Won-Jin (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ro, Younghye (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ko, Dae-Sung (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Nam-Hyung (Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Jae-Hong (Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Kwon, Hyuk-Joon (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
  • 김단일 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 김은경 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 성원진 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 노영혜 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 고대성 (서울대학교 수의과대학 산업동물의학연구실) ;
  • 김남형 (서울대학교 수의과대학 조류질병학연구실) ;
  • 김재홍 (서울대학교 수의과대학 조류질병학연구실) ;
  • 권혁준 (서울대학교 수의과대학 산업동물의학연구실)
  • Received : 2017.02.16
  • Accepted : 2017.05.11
  • Published : 2017.06.30

Abstract

Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.

Acknowledgement

Supported by : 농촌진흥청

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