Optimization of Extraction Process for Antioxidant from Persimmon Leaf and Thistle Using Response Surface Methodology

반응표면분석법을 이용한 감잎과 엉겅퀴로부터 항산화성분의 추출공정 최적화

  • Lee, Seung Bum (Department of Chemical Engineering, Dankook University) ;
  • Jang, Hyun Sick (Department of Chemical Engineering, Dankook University) ;
  • Hong, In Kwon (Department of Chemical Engineering, Dankook University)
  • Received : 2017.04.28
  • Accepted : 2017.06.09
  • Published : 2017.08.10


In this study, we extracted an antioxidant from natural products which are known to have a high antioxidant content and also optimized the extraction process by applying a response surface methodology (RSM). In addition, by measuring the total flavonoids and total polyphenols of the active ingredient extracted persimmon leaf and thistle, the functionality of the active ingredient was evaluated. Both ultrapure water and alcohol were used as extraction solvents and the ratio of ultrapure and alcohol, amount of sample, extraction time, extraction temperature were set as independence variables. Also, the yield, total polyphenols, and flavonoids was set as the response. Optimal extraction conditions were as follows; for persimmon leaf, the extraction time = 3.1 h, ratio of alcohol/ultrapure = 63.4 vol%, and temperature = $54.6^{\circ}C$ while for thistle the extraction time = 2.9 h, ratio of alcohol/ultrapure = 40.7 vol%, and temperature = $68.4^{\circ}C$. Also, the response were as follows; for persimmon leaf, the yield = 27.7%, total polyphenols = 33.2 mg GAE/g, and total flavonoids = 47.8 mg QE/mg dw, whereas for thistle the yield = 27.0%, total polyphenols = 17.9 mg GAE/g, and total flavonoids = 28.8 mg QE/mg dw at the optimal conditions. The overall satisfaction level was 71.7%.


Supported by : 단국대학교


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