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Optimization of Extraction Process for Antioxidant from Persimmon Leaf and Thistle Using Response Surface Methodology

반응표면분석법을 이용한 감잎과 엉겅퀴로부터 항산화성분의 추출공정 최적화

  • Lee, Seung Bum (Department of Chemical Engineering, Dankook University) ;
  • Jang, Hyun Sick (Department of Chemical Engineering, Dankook University) ;
  • Hong, In Kwon (Department of Chemical Engineering, Dankook University)
  • Received : 2017.04.28
  • Accepted : 2017.06.09
  • Published : 2017.08.10

Abstract

In this study, we extracted an antioxidant from natural products which are known to have a high antioxidant content and also optimized the extraction process by applying a response surface methodology (RSM). In addition, by measuring the total flavonoids and total polyphenols of the active ingredient extracted persimmon leaf and thistle, the functionality of the active ingredient was evaluated. Both ultrapure water and alcohol were used as extraction solvents and the ratio of ultrapure and alcohol, amount of sample, extraction time, extraction temperature were set as independence variables. Also, the yield, total polyphenols, and flavonoids was set as the response. Optimal extraction conditions were as follows; for persimmon leaf, the extraction time = 3.1 h, ratio of alcohol/ultrapure = 63.4 vol%, and temperature = $54.6^{\circ}C$ while for thistle the extraction time = 2.9 h, ratio of alcohol/ultrapure = 40.7 vol%, and temperature = $68.4^{\circ}C$. Also, the response were as follows; for persimmon leaf, the yield = 27.7%, total polyphenols = 33.2 mg GAE/g, and total flavonoids = 47.8 mg QE/mg dw, whereas for thistle the yield = 27.0%, total polyphenols = 17.9 mg GAE/g, and total flavonoids = 28.8 mg QE/mg dw at the optimal conditions. The overall satisfaction level was 71.7%.

Acknowledgement

Supported by : 단국대학교

References

  1. M. Y. Lee, M. S. Yoo, Y. J. Whang, Y. J. Jin, M. H. Hong, and Y. H. Pyo, Vitamin C, total polyphenol, flavonoid contents and antioxidant capacity of several fruit peels, Korean J. Food Sci. Technol., 44(5), 540-544 (2012). https://doi.org/10.9721/KJFST.2012.44.5.540
  2. L. Van-Gaal, I. Mertens, and C. De-Block, Mechanisms linking obesity with cardiovascular disease, Nature, 444, 875-880 (2006). https://doi.org/10.1038/nature05487
  3. S. A. Park, J. H. Ha, and S. N. Park, Antioxidative activity and component analysis of Broussonetia kazinoki SIEB extracts, Appl. Chem. Eng., 24(2), 177-183 (2013).
  4. H. S. Jeong and J.-H. Lee, Effects of dietary fiber from mozuku (cladosiphon novae-caledoniae kylin) residue on antioxidant activity and anticancer in HT-29 human colon cancer cells according to extraction condition, Appl. Chem. Eng., 25(4), 363-367 (2014). https://doi.org/10.14478/ace.2014.1039
  5. A. H. Clifford and S. L. Cuppett, Anthocyanins-nature, occurrence and dietary burden, J. Sci. Food Agric., 80, 1063-1072 (2000). https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1063::AID-JSFA605>3.0.CO;2-Q
  6. T. Belwal, P. Dhyani, I. D. Bhatt, R. S. Rawal, and V. Pande, Optimization extraction conditions for improving phenolic content and antioxidant activity in Berberis asiatica fruits using response surface methodology (RSM), Food Chem., 207, 115-124 (2016). https://doi.org/10.1016/j.foodchem.2016.03.081
  7. A. Alberti, A. A. F. Zelinski, D. M. Zardo, I. M. Demiate, A. Nogueira, and L. I. Mafra, Extraction of flavonoids from bitter melon, Food Chem., 149, 151-158 (2014). https://doi.org/10.1016/j.foodchem.2013.10.086
  8. N. Ilaiyaraja, K. R. Likhith, G. R. Sharath Babu, and F. Khanum, Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM), Food Chem., 173, 348-354 (2015). https://doi.org/10.1016/j.foodchem.2014.10.035
  9. A. A. D'Archivio and M. A. Maggi, Investigation by response surface methodology of the combined effect of pH and composition of water-methanol mixtures on the stability of curcuminoids., Food Chem., 219, 414-418 (2017). https://doi.org/10.1016/j.foodchem.2016.09.167
  10. M. S. Parco, Y. Wang, and E. A. Stephen, Apoptotic signaling induced by $H_2O_2$-mediated oxidative stress in differentiated $C_2C_{12}$ myotubes, Life Sci., 84(13-14), 468-481 (2009). https://doi.org/10.1016/j.lfs.2009.01.014
  11. A. H. Clifford and S. L. Cuppett, Anthocyanins-nature, occurrence and dietary burden, J. Sci. Food Agric., 80, 1063-1072 (2000). https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1063::AID-JSFA605>3.0.CO;2-Q
  12. N. C. Cook and S. Samman, Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources, J. Nutr. Biochem., 7, 66-76 (1996). https://doi.org/10.1016/0955-2863(95)00168-9
  13. S. Beck and J. Stengel, Mass spectrometric imaging of flavonoid glycosides and biflavonoids in Ginkgo biloba L., Phytochemistry, 130, 201-206 (2016). https://doi.org/10.1016/j.phytochem.2016.05.005
  14. R. F. Yanga, L. L. Genga, H. Q. Lub, and X. D. Fanc, Ultrasound-synergized electrostatic field extraction of total flavonoids from Hemerocallis citrina baroni, Ultrason. Sonochem., 34, 571-579 (2017). https://doi.org/10.1016/j.ultsonch.2016.06.037
  15. I. K. Hong, B. R. Park, G. S. Jeon, and S. B. Lee, Extraction of flavonoid components from persimmon leaf, thistle and new green, Appl. Chem. Eng., 27(3), 276-279 (2016). https://doi.org/10.14478/ace.2016.1027