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Cellular Protective Effect and Active Component Analysis of Lavender (Lavandula angustifolia) Extracts and Fractions

라벤더(Lavandula angustifolia) 추출물 및 분획물의 세포보호효과와 활성 성분 분석

  • Kim, A Young (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Ha, Ji Hoon (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kim, A Rang (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Jeong, Hyo Jin (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Kim, Kyoung Mi (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 김아영 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 하지훈 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 김아랑 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 정효진 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 김경미 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소)
  • Received : 2017.05.25
  • Accepted : 2017.07.05
  • Published : 2017.08.10

Abstract

In this study, antioxidative activities and cellular protective effects of 70% ethanol extracts and fractions from lavender were evaluated. The scavenging activity ($FSC_{50}$) of free radical (1,1-phenyl-2-picrylhydrazyl, DPPH) was 46.6, 45.5 and $477.5{\mu}g/mL$ in the 70% ethanol extract, ethyl acetate fraction and aglycone fraction, respectively. The reactive oxygen species scavenging activities (${OSC_{50}$) of 70% ethanol extract, ethyl acetate fraction and aglycone fraction were 8.1, 3.3 and $17.6{\mu}g/mL$, respectively, and they showed lower antioxidative activity than that of using L-ascorbic acid ($1.5{\mu}g/mL$). However, the aglycone fraction showed higher photohemolysis protective effect than that of using the 70% ethanol extract and ethyl acetate fraction. At $50{\mu}M$ concentration, the cellular protective effect (${\tau}_{50}$) of 70% ethanol extract, ethyl acetate fraction and aglycone fraction from lavender was 70.6, 87.2 and 165.2 min, respectively. In particular, the lavender aglycone fraction showed 3.8 times higher cellular protective effect than that of (+)-${\alpha}$-tocopherol. The lavender fractional components including luteolin 7-O-glucuronide, vitextin, rosmarinic acid, luteolin, and apigenin were identified using TLC and LC-MS. However, the lavender aglycone fraction did not show any significant increase in flavonoids (luteolin and apigenin) compared to that of the ethyl acetate fraction. In conclusion, it is suggested that lavender may be applied as an antioxidant material in cosmetic industries.

Acknowledgement

Supported by : 보건복지부

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