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Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis

  • Yang, Dong-Kun (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Kim, Ha-Hyun (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Yoo, Jae-Young (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Lim, Suk-Kyung (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Yoon, Soon-Seek (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs) ;
  • Cho, In-Soo (Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs)
  • Received : 2017.07.03
  • Accepted : 2017.08.10
  • Published : 2017.09.30

Abstract

A Gram-negative, catalase- and oxidase-positive, coccus-shaped bacterium was isolated from a rabbit with keratoconjunctivitis. Colonies of the isolate were round, smooth, and exhibited hemolytic activity on 5% sheep blood agar. Scanning electron microscopy revealed 0.4 to $0.5{\mu}m$ diameter oval cocci. Partial 16S rRNA gene (1446 bp) sequence analysis demonstrated the isolate had significant homology with the Moraxella cuniculi CCUG2154 strain isolated from a rabbit in Germany in 1973. Our isolate was designated as APQAB1701. Antibiotic susceptibility tests demonstrated that APQAB1701 was sensitive to 24 antibiotics; 3 of the antibiotics (nalidixic acid, spectinomycin, and colistin) had minimal inhibitory concentrations ${\geq}32{\mu}g/mL$ against the isolate.

Acknowledgement

Supported by : Animal and Plant Quarantine Agency

References

  1. Farajzadah Sheikh A, Saki N, Roointan M, Ranjbar R, Yadyad MJ, Kaydani A, Aslani S, Babaei M, Goodarzi H. Identification of Alloiococcus otitidis, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in children with otitis media with effusion. Jundishapur J Microbiol 2015, 8, e17985.
  2. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23, 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  3. Maayan H, Cohen-Poradosu R, Halperin E, Rudensky B, Schlesinger Y, Yinnon AM, Raveh D. Infective endocarditis due to Moraxella lacunata: report of 4 patients and review of published cases of Moraxella endocarditis. Scand J Infect Dis 2004, 36, 878-881. https://doi.org/10.1080/00365540410024925
  4. Pugh GW Jr, McDonald TJ. Identification of bovine carriers of Moraxella bovis by comparative cultural examinations of ocular and nasal secretions. Am J Vet Res 1986, 47, 2343-2345.
  5. Sosa V, Umpierrez A, Acquistapace S, Zunino P. Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases. J Infect Dev Ctries 2015, 9, 1028-1032. https://doi.org/10.3855/jidc.6222
  6. Sung JY, Hong SK, Kim EC. The first Korean case of Moraxella osloensis bacteremia in a patient with acute myeloid leukemia. Ann Lab Med 2014, 34, 256-258. https://doi.org/10.3343/alm.2014.34.3.256
  7. Tamang MD, Dey S, Makaju RK, Jha BK, Shivananda PG, Bhramadatan KN. Prevalence of Moraxella catarrhalis infections of the lower respiratory tract in elderly patients. Kathmandu Univ Med J (KUMJ) 2005, 3, 39-44.
  8. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  9. Vela AI, Arroyo E, Aragon V, Sanchez-Porro C, Latre MV, Cerda-Cuellar M, Ventosa A, Dominguez L, Fernandez-Garayzabal JF. Moraxella pluranimalium sp. nov., isolated from animal specimens. Int J Syst Evol Microbiol 2009, 59, 671-674. https://doi.org/10.1099/ijs.0.006205-0
  10. Yeruham I, Perl S, Elad D. Infectious bovine keratoconjunctivitis and lymphofollicular hyperplasia of the third eyelid in heifers. J Vet Med B Infect Dis Vet Public Health 2001, 48, 137-141. https://doi.org/10.1046/j.1439-0450.2001.00428.x