Sargassum yezoense Extract Inhibits Carbohydrate Digestive Enzymes In Vitro and Alleviates Postprandial Hyperglycemia in Diabetic Mice.

  • Park, Jae-Eun (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Ji-Hee (Department of Food Science and Nutrition, Pusan National University) ;
  • Han, Ji-Sook (Department of Food Science and Nutrition, Pusan National University)
  • Received : 2017.03.07
  • Accepted : 2017.04.21
  • Published : 2017.09.30


In this study, we investigated whether Sargassum yezoense extract (SYE) could inhibit ${\alpha}-glucosidase$ and ${\alpha}-amylase$ activities, and alleviate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. Freeze-dried S. yezoense was extracted with 80% ethanol and concentrated for use in this study. The hypoglycemic effect was determined by evaluating the inhibitory activities of SYE against ${\alpha}-glucosidase$ and ${\alpha}-amylase$ as well as its ability to decrease postprandial blood glucose levels. The half-maximal inhibitory concentrations of SYE against ${\alpha}-glucosidase$ and ${\alpha}-amylase$ were $0.078{\pm}0.004$ and $0.212{\pm}0.064mg/mL$, respectively. SYE was a more effective inhibitor of ${\alpha}-glucosidase$ and ${\alpha}-amylase$ activities than the positive control, acarbose. The increase in postprandial blood glucose levels was significantly alleviated in the SYE group compared with that in the control group of STZ-induced diabetic mice. Furthermore, the area under the curves significantly decreased with SYE administration in STZ-induced diabetic mice. These results suggest that SYE is a potent inhibitor of ${\alpha}-glucosidase$ and ${\alpha}-amylase$ activities and alleviates postprandial hyperglycemia caused by dietary carbohydrates.


Sargassum yezoense;${\alpha}-glucosidase$;${\alpha}-amylase$;postprandial hyperglycemia;diabetic mice


Supported by : Pusan National University


  1. Zimmet P, Alberti KGMM, Shaw J. 2001. Global and societal implications of the diabetes epidemic. Nature 414: 782-787.
  2. Muoio DM, Newgard CB. 2006. Obesity-related derangements in metabolic regulation. Annu Rev Biochem 75: 367-401.
  3. Baron AD. 1998. Postprandial hyperglycaemia and ${\alpha}$-glucosidase inhibitors. Diabetes Res Clin Pract 40: S51-S55.
  4. Ceriello A. 2005. Postprandial hyperglycemia and diabetes complications: is it time to treat?. Diabetes 54: 1-7.
  5. UK Prospective Diabetes Study (UKPDS) Group. 1998. Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33). Lancet 352: 837-853.
  6. Saito N, Sakai H, Suzuki S, Sekihara H, Yajima Y. 1998. Effect of an ${\alpha}$-glucosidase inhibitor (voglibose), in combination with sulphonylureas, on glycaemic control in type 2 diabetes patients. J Int Med Res 26: 219-232.
  7. Lebovitz HE. 2002. Treating hyperglycemia in type 2 diabetes: new goals and strategies. Cleve Clin J Med 69: 809-820.
  8. Carroll MF, Gutierrez A, Castro M, Tsewang D, Schade DS. 2003. Targeting postprandial hyperglycemia: a comparative study of insulinotropic agents in type 2 diabetes. J Clin Endocrinol Metab 88: 5248-5254.
  9. Fonseca V. 2003. Clinical significance of targeting postprandial and fasting hyperglycemia in managing type 2 diabetes mellitus. Curr Med Res Opin 19: 635-641.
  10. Hanefeld M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J Diabetes Complications 12: 228-237.
  11. Yuan YV, Walsh NA. 2006. Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol 44: 1144-1150.
  12. Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, Hong YK. 2008. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacol 116: 187-190.
  13. Pushpamali WA, Nikapitiya C, De Zoysa M, Whang I, Kim SJ, Lee J. 2008. Isolation and purification of an anticoagulant from fermented red seaweed Lomentaria catenata. Carbohydr Polym 73: 274-279.
  14. Kwon MJ, Nam TJ. 2006. Porphyran induces apoptosis related signal pathway in AGS gastric cancer cell lines. Life Sci 79: 1956-1962.
  15. Hong IS, Kim GA, Park JK, Boo SM. 2008. Morphology and phenology of Sargassum yezoense (Sargassaceae, Phaeophyceae). Korean Journal of Nature Conservation 2: 132-139.
  16. Nakai M, Kageyama, N, Nakahara K, Miki W. 2006. Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum. Mar Biotechnol 8: 409-414.
  17. Reddy P, Urban S. 2009. Meroditerpenoids from the southern Australian marine brown alga Sargassum fallax. Phytochemistry 70: 250-255.
  18. Jung M, Jang KH, Kim B, Lee BH, Choi BW, Oh KB, Shin J. 2008. Meroditerpenoids from the brown alga Sargassum siliquastrum. J Nat Prod 71: 1714-1719.
  19. Seo Y, Park KE, Kim YA, Lee HJ, Yoo JS, Ahn JW, Lee BJ. 2006. Isolation of tetraprenyltoluquinols from the brown alga Sargassum thunbergii. Chem Pharm Bull 54: 1730-1733.
  20. Kim SN, Choi HY, Lee W, Park GM, Shin WS, Kim YK. 2008. Sargaquinoic acid and sargahydroquinoic acid from Sargassum yezoense stimulate adipocyte differentiation through $PPAR{\alpha}$/${\gamma}$ activation in 3T3-L1 cells. FEBS Lett 582: 3465-3472.
  21. Watanabe J, Kawabata J, Kurihara H, Niki R. 1997. Isolation and identification of ${\alpha}$-glucosidase inhibitors from tochucha (Eucommia ulmoides). Biosci Biotechnol Biochem 61: 177-178.
  22. Zheng J, He J, Ji B, Li Y, Zhang X. 2007. Antihyperglycemic activity of Prunella vulgaris L. in streptozotocin-induced diabetic mice. Asia Pac J Clin Nutr 16: 427-431.
  23. Kim JS. 2004. Effect of Rhemanniae Radix on the hyperglycemic mice induced with streptozotocin. J Korean Soc Food Sci Nutr 33: 1133-1138.
  24. Prashanth D, Padmaja R, Samiulla DS. 2001. Effect of certain plant extracts on ${\alpha}$-amylase activity. Fitoterapia 72: 179-181.
  25. Lee EH, Ham J, Ahn HR, Kim MC, Kim CY, Pan CH, Um BH, Jung SH. 2009. Inhibitory effects of the compounds isolated from Sargassum yezoense on ${\alpha}$-glucosidase and oxidative stress. Kor J Pharmacogn 40: 150-154.
  26. Lebovitz HE. 1992. Oral antidiabetic agents: the emergence of ${\alpha}$-glucosidase inhibitors. Drugs 3: 21-28.
  27. Tadera K, Minami Y, Takamatsu K, Matsuoka T. 2006. Inhibition of ${\alpha}$-glucosidase and ${\alpha}$-amylase by flavonoids. J Nutr Sci Vitaminol 52: 149-153.
  28. Koskinen P, Manttari M, Manninen V, Huttunen JK, Heinonen OP, Frick MH. 1992. Coronary heart disease incidence in NIDDM patients in the Helsinki Heart Study. Diabetes Care 15: 820-825.
  29. Ceriello A, Davidson J, Hanefeld M, Leiter L, Monnier L, Owens D, Tajima N, Tuomilehto J; International Prandial Glucose Regulation Study Group. 2006. Postprandial hyperglycaemia and cardiovascular complications of diabetes: an update. Nutr Metab Cardiovasc Dis 16: 453-456.
  30. Inoue I, Takahashi K, Noji S, Awata T, Negishi K, Katayama S. 1997. Acarbose controls postprandial hyperproinsulinemia in non-insulin dependent diabetes mellitus. Diabetes Res Clin Pract 36: 143-151.
  31. Stern JL, Hagerman AE, Steinberg PD, Mason PK. 1996. Phlorotannin-protein interactions. J Chem Ecol 22: 1877-1899.