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Fungicide selections for control of chili pepper stem rot caused by Sclerotium rolfsii using an agar dilution method

  • Lee, Soo Min (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Min, Jiyoung (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • Received : 2017.06.01
  • Accepted : 2017.06.28
  • Published : 2017.09.30

Abstract

Sclerotium rolfsii causing southern blight on numerous vegetable and fruit crops was isolated from stems of chili peppers showing wilting symptoms. The pathogen was identified by morphological observation and DNA sequencing analysis of ITS region. To select an effective fungicide for control of southern blight, we investigated the inhibition efficacy of thirty fungicides included in nine groups of fungicides with different mechanisms of action. A fungal growth inhibition assay was conducted through an agar dilution method by using mycelial discs and sclerotia of the pathogen as inoculum, respectively. When mycelial discs were used as an inoculum, several fungicides showed good inhibitory activity against the mycelial growth of S. rolfsii 12-6. All DMI fungicides tested had a good inhibition except for prochloraz which had low inhibitory effect. All strobilurin fungicides tested except for kresoxim-methyl and all SDHI fungicides tested except for boscalid and fluopyram, had a good inhibition. Also, fludioxonil, a protective fungicide and fluazinam had a good inhibitory effect. Interestingly, when sclerotia were used as an inoculum, inhibition efficacy was increased for fluopyram, a SDHI fungicide, and for some protective fungicides such as propineb, chlorothalonil, dithianon, and folpet. All the fungicides selected in this study should be tested in the field for their control activities against stem rot for practical use in chili pepper cultivation.

Acknowledgement

Grant : 지역 맞춤형 노지 고추의 주요 병해 종합적 방제 체계 구축

Supported by : 농촌진흥청

References

  1. Boukaew S. Chuenchit S, Petcharat V. 2011. Evaluation of Streptomyces spp. for biological control of Sclerotium root and stem rot and Ralstonia wilt of chili pepper. Biocontrol 56:365-374. https://doi.org/10.1007/s10526-010-9336-4
  2. Duffand JD, Connelly MI. 1993. Effect of solarisation using single and double layers of clear plastic mulch on Pythium, Phytophthora and Sclerotium species in a nursery potting mix. Australasian Plant Pathology 22:28-35. https://doi.org/10.1071/APP9930028
  3. Eslami AA, Khodaparast SA, Mousanejad S, Dehkaei FP. 2015. Evaluation of the virulence of Sclerotium rolfsii isolates on Arachis hypogaea and screening for resistant genotypes in greenhouse conditions. Hellenic Plant Protection Journal 8:1-11. https://doi.org/10.1515/hppj-2015-0001
  4. Fouzia Y, Saleem S. 2006. Effect of fungicides on in vitro growth of Sclerotium rolfsii. Pakistan Journal Botany 38:881-883.
  5. Fouzia Y, Saleem S. 2009. Effect of solar heating by polyethylene mulching on sclerotial viability and pathogenicity of Sclerotium rolfsii on mungbean and sunflower. Pakistan Journal Botany 41:3199-3205.
  6. Ishikawa R, Shirouzu K, Nakashita H, Lee HY, Motoyama T, Yamaguchi I, Teraoka T, Arie T. 2005. Foliar spray of validamycin A or validoxylamine A controls tomato Fusarium wilt. Phytopathology 95:1209-1216. https://doi.org/10.1094/PHYTO-95-1209
  7. Kim AH, Kim SB, Han KD, Kim HT. 2014. Monitoring for the resistance of strobilurin fungicide against Botrytis cinerea causing gray mold disease. The Korean Journal of Pesticide Science 18:161-167. https://doi.org/10.7585/kjps.2014.18.3.161
  8. Kim JJ, Kim JT, Park SW, Park ES, Kim HT. 2003. Development of assay method for the activities of new compounds, and the effect of several fungicides against spore germination, adhesion and mycelial growth of Colletotrichum sp. causing red pepper anthracnose. The Korean Journal of Pesticide Science 7:159-168.
  9. Kwon JH, Kang DW, Lee ST, Choi O, Shen SS. 2011. Stem rot of Stachys sieboldii caused by Sclerotium rolfsii. Research in Plant Disease 17:399-401. [in Korean] https://doi.org/10.5423/RPD.2011.17.3.399
  10. Kwon JH, Kim H, Lee YH, Shim HS. 2012. Sclerotium rot of sponge gourd caused by Sclerotium rolfsii. Research of Plant Disease 18:54-56. [in Korean] https://doi.org/10.5423/RPD.2012.18.1.054
  11. Kwon JH, Park CS 2004. Stem rot of Capsocum annuum caused by Sclerotium rolfsii in Korea. Research of Plant Disease 10:21-24. [in Korean] https://doi.org/10.5423/RPD.2004.10.1.021
  12. Kwon JH, Park CS 2009. Occurrence of fruit rot of watermelon (Citrullus lanatus) caused by Sclerotium rolfsii. Research of Plant Disease 15:51-53. [in Korean] https://doi.org/10.5423/RPD.2009.15.1.051
  13. Leoni C, Braak CJF, Gilsanz JC, Dogliotti S, Rossing WAH, van Bruggen AHC. 2014. Sclerotium rolfsii dynamics in soil as affected by crop sequences. Appled Soil and Ecology 75:95-105. https://doi.org/10.1016/j.apsoil.2013.11.002
  14. McGovern RJ. 2015. Management of tomato diseases caused by Fusarium oxysporum. Crop Protection 73:78-92. https://doi.org/10.1016/j.cropro.2015.02.021
  15. Stevens C, Khan VA, Rodriguez-Kabana R, Ploper LD, Backman PA, Collins DJ, Brown JE, Wilson MA, Igwegbe ECK. 2003. Integration of soil solarization with chemical, biological and cultural control for the management of soilborne diseases of vegetables. Plant and Soil 253:493-506. https://doi.org/10.1023/A:1024895131775