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Effect of Ipomoea aquatica extract on anti-melanogenesis and skin barrier function

공심채 추출물의 멜라닌 생성 저해 및 피부장벽 개선 효과

  • Kim, Hyun-Soo (Department of Food Science and Technology, Jungwon University)
  • 김현수 (중원대학교 식품공학과)
  • Received : 2017.04.13
  • Accepted : 2017.05.29
  • Published : 2017.10.31

Abstract

We investigated the applications of functional materials through the examination of a variety of physiological activities of Ipomoea aquatica extract. I. aquatica extract showed low cytotoxicity against murine melanoma B16F10 cells. At concentrations that exerted little or no cytotoxicity to the cells, I. aquatica extract showed high DPPH radical scavenging activity ($ID_{50}$, $7.84{\mu}g/mL$), inhibited tyrosinase activity ($ID_{50}$, $106.56{\mu}g/mL$), and decreased melanin content ($ID_{50}$, $41.75{\mu}g/mL$). The treatment of B16F10 cells with I. aquatica extract suppressed the protein expression of tyrosinase in a dose-dependent manner. These findings suggested that I. aquatica extract inhibited melanin synthesis in murine melanoma B16F10 cells through the suppression of intracellular tyrosinase expression, as well as the simultaneous direct inhibition of tyrosinase activity. Additionally, I. aquatica extract promoted the expression of involucrin, which is related to skin barrier protection. These results indicate that I. aquatica extract may be an appropriate material for the improvement of skin barrier function.

Keywords

Ipomoea aquatica;melanin;tyrosinase;involucrin

References

  1. Elias PM. Stratum corneum defensive functions: An integrated view. J. Invest. Dermatol. 125: 183-200 (2005) https://doi.org/10.1111/j.0022-202X.2005.23668.x
  2. Holleran WM, Takagi Y, Menon GK, Jackson SM, Lee JM, Feingold KR, Elias PM. Permeability barrier requirements regulate epidermal beta-glucocerebrosidase. J. Lipid Res. 35: 905-912 (1994)
  3. Downing DT. Lipid and protein structures in the permeability barrier of mammalian epidermis. J. Lipid Res. 33: 301-313 (1992)
  4. Kuenzli S, Saurat JH. Peroxisome proliferator-activated receptors in cutaneous biology. Br. J. Dermatol. 149: 229-236 (2003) https://doi.org/10.1046/j.1365-2133.2003.05532.x
  5. Briganti S, Camera E, Picardo M. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell. Res. 16: 101-110 (2003) https://doi.org/10.1034/j.1600-0749.2003.00029.x
  6. Lamy E, Schrder J, Paulus S, Brenk P, Stahl T, Mersch-Sundermann V. Antigenotoxic properties of Eruca sativa (rocket plant), erucin and erysolin in human hepatoma (HepG2) cells towards benzo(a)pyrene and their mode of action. Food Chem. Toxicol. 46: 2415-2421 (2008) https://doi.org/10.1016/j.fct.2008.03.022
  7. Dua TK, Dewanjee S, Gangopadhyay M, Khanra R, Zia-Ul-Haq M. Ameliorative effect of water spinach, Ipomoea aquatic (Convolvulaceae), against experimentally induced arsenic toxicity. J Transl. Med. 13: 81-98 (2015) https://doi.org/10.1186/s12967-015-0430-3
  8. Malalavidhane TS, Wickramasinghe SM, Perera MS, Jansz ER. Oral hypoglycaemic activity of Ipomoea aquatic in streptozotocin-induced, diabetic wistar rats and type II diabetics. Phytother. Res. 17: 1098-1100 (2003) https://doi.org/10.1002/ptr.1345
  9. Badruzzaman SM, Husain W. Some aquatic and marshy land medicinal plants from Hardoi district of uttar Pradesh. Fitoterapia 63: 245-247 (1992)
  10. Samuelsson G, Farah MH, Claeson P, Hagos M, Thulin M, Hedberg O, Warfa AM, Hassan AO, Elmi AH, Abdurahman AD. Inventory of plants used in traditional medicine in Somalia. II. Plants of the families combretaceae to labiatae. J. Ethnopharmacol. 37: 47-70 (1992) https://doi.org/10.1016/0378-8741(92)90004-B
  11. Chopra RN, Nayar SL, Chopra IC. Glossary oof indian medicinal plants. Council of Scientific and Industrial Research, New Delhi, India (1956)
  12. Kim B, Choi YE, Kim HS. Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator-activated receptor (PPAR)-${\alpha}$ and suppression of inflammatory cytokines. Phytother. Res. 28: 1359-1366 (2014) https://doi.org/10.1002/ptr.5138
  13. Ranke J, Mlter K, Stock F, Bottin-Weber U, Poczobutt J, Hoffmann J, Ondruschka B, Filser J, Jastorff B. Biological effects of imidazolium ionic liquids with varying chain lengths in acute Vibrio fischeri and WST-1 cell viability assays. Ecotoxicol. Environ. Saf. 58: 396-404 (2004) https://doi.org/10.1016/S0147-6513(03)00105-2
  14. Miliauskas G, Venskutonis PR, van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 85: 231-237 (2004) https://doi.org/10.1016/j.foodchem.2003.05.007
  15. Masamoto Y, Ando H, Murata Y, Shimoishi Y, Tada M, Takahata K. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L. Biosci. Biotechnol. Biochem. 67: 631-634 (2003) https://doi.org/10.1271/bbb.67.631
  16. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478 (1985)
  17. Jeon YS, Jung YJ, Youm JK, Kim YK, Kim SN. Inhibitory effect of Endarachne binghamiae extract on melanin synthesis. Korean J. Plant Res. 26: 526-532 (2013) https://doi.org/10.7732/kjpr.2013.26.5.526
  18. Kim TH, You JK, Kim JM, Baek JM, Kim HS, Park JH, Choe M. Antioxidant and whitening effects of Sorbus commixta HEDL cortex extract. J. Korean Soc. Food Sci. Nutr. 39: 1418-1424 (2010) https://doi.org/10.3746/jkfn.2010.39.10.1418
  19. Song HS, Moon HJ, Park BE, Choi BS, Lee DJ, Lee JY, Kim CJ, Sim SS. Anti-oxidant activity and whitening activity of bamboo extracts. J. Pharm. Soc. 51: 500-507 (2007)
  20. Yoon HS, Yang KW, Kim JE, Kim JM, Lee NH, Hyun CG. Hypopigmenting effects of extracts from bulbs of Lilium otriental hybrid 'Siberia' in murine B16/F10 melanoma cells. J. Korean Soc. Food Sci. Nutr. 43: 705-711 (2014) https://doi.org/10.3746/jkfn.2014.43.5.705
  21. Kim B, Lee SH, Choi KY, Kim HS. N-nicotinoyl tyramine, a novel niacinamide derivative, inhibits melanogenesis by suppressing MITF gene expression. Eur. J. Pharmacol. 764: 1-8 (2015) https://doi.org/10.1016/j.ejphar.2015.05.006