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Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs

  • Lee, Jae-Bong (Korea Zoonosis Research Institute (KoZRI), Chonbuk National University) ;
  • Park, Hee-Bok (Subtropical Livestock Research Institute, National Institute of Animal Science) ;
  • Yoo, Chae-Kyoung (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hee-Sung (Division of Applied Life Science (BK21 plus), Gyeongsang National University) ;
  • Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science) ;
  • Lim, Hyun-Tae (Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2016.05.30
  • Accepted : 2016.12.16
  • Published : 2017.08.01

Abstract

Objective: Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 (SW1482-SW963) in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 (GRIP1) as a positional candidate gene underlying the QTL affecting BFT traits. Methods: Genotype and phenotype analyses were performed using the 1,105 $F_2$ progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the $F_2$ intercross population. Results: Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in GRIP1 with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits. Conclusion: The SNPs and haplotypes of the GRIP1 gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.

Keywords

Glutamate Receptor-interacting Protein 1 (GRIP1);Backfat;Quantitative Trait Loci (QTL);Landrace;Korean Native Pigs

Acknowledgement

Supported by : Rural Development Administration

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