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Polymorphism in the intron 20 of porcine O-linked N-acetylglucosamine transferase

  • Kim, Jong Gug (Department of Animal Sciences, College of Agriculture and Life Science, and Institute of Molecular Biology and Genetics, Chonbuk National University) ;
  • Nonneman, Dan (U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center) ;
  • Kim, Doo-Wan (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Shin, Sangsu (Department of Animal Biotechnology, Kyungpook National University) ;
  • Rohrer, Gary A. (U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center)
  • Received : 2017.03.01
  • Accepted : 2017.05.22
  • Published : 2017.08.01

Abstract

Objective: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of O-GlcNAc and GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling and transcription. Pig OGT is located near the region of chromosome X that affects follicle stimulating hormone level and testes size. The objective of this study was to find the variations of OGT between European and Chinese pigs. Methods: Pigs were tested initially for polymorphism in OGT among European and Chinese pigs by polymerase chain reaction and sequencing at the U.S. Meat Animal Research Center (USMARC). The polymorphism was also determined in an independent population of pigs including European and Chinese Meishan (ME) breeds at the National Institute of Animal Science (NIAS, RDA, Korea). Results: The intron 20 of OGT from European and Chinese pigs was 514 and 233 bp, respectively, in the pigs tested initially. They included 1 White composite (WC) boar and 7 sows ($2Minzu{\times}WC$, $2Duroc\;[DU]{\times}WC$, $2ME{\times}WC$, $1Fengzing{\times}WC$) at USMARC. The 281-bp difference was due to an inserted 276-bp element and GACTT in European pigs. When additional WC and ME boars, the grandparents that were used to generate the $1/2ME{\times}1/2WC$ parents, and the 84 boars of 16 litters from mating of $1/2ME{\times}1/2WC$ parents were analyzed, the breeds of origin of X chromosome quantitative trait locus (QTL) were confirmed. The polymorphism was determined in an independent population of pigs including DU, Landrace, Yorkshire, and ME breeds at NIAS. OGT was placed at position 67 cM on the chromosome X of the USMARC swine linkage map. Conclusion: There was complete concordance with the insertion in European pigs at USMARC and NIAS. This polymorphism could be a useful marker to identify the breed of origin of X chromosome QTL in pigs produced by crossbreeding Chinese and European pigs.

Keywords

Breeds of Origin;Pig;O-linked N-acetylglucosamine Transferase;Polymorphism;X chromosome

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