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Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers

  • Peng, Dong Qiao (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Jung, U Suk (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, Jae Sung (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, Won Seob (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Jo, Yong Ho (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, Min Jeong (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Oh, Young Kun (Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA) ;
  • Baek, Youl Chang (Animal Nutrition and Physiology team, Department of Animal Biotechnology and Environment, National Institute of Animal Science RDA) ;
  • Hwang, Seong Gu (Department of Animal Life and Environment Science, Hankyong National University) ;
  • Lee, Hong Gu (Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University)
  • Received : 2016.09.19
  • Accepted : 2017.01.11
  • Published : 2017.08.01

Abstract

Objective: This work was to find the correlation of alcohol dehydrogenase 1C (ADH1C) genotype with vitamin A reduction and carcass traits during the vitamin A restriction period. Methods: In study 1, 60 Korean native steers were fed a diet (890 IU/kg) with 8,000 IU and 0 IU of supplemental premix vitamin A/kg of dry matter (DM) for control and treatment group, respectively. The levels of serum vitamin A were analyzed through high preparative performance liquid chromatography, and the ADH1C genotype was analyzed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP; 78.1% TT type, 21.9% TC type); however, CC type was not found. Then, the interaction between ADH1C and carcass traits on the vitamin A restriction was investigated in study 2. A total of 136 Korean native steers were fed a diet that included 930 IU/kg vitamin A of DM. Results: Serum vitamin A in treatment was reduced to 112.4 IU/dL in steers with TT type of ADH1C, while for steers with TC type the concentration of serum vitamin A was dropped to 79.5 IU/dL (p<0.1) in study 1. This showed that TC type had the potential to lower serum vitamin A concentration during vitamin A restriction compared to TT type. In study 2 we found that eye muscle area, marbling and carcass weight in Korean native steers with TC type were higher than in steers with TT type (p<0.05). Conclusion: The interaction between vitamin A restriction and TC type of ADH1C gene could have the potential of increasing the marbling in Korean native steers. These results indicated that steers with TC type of the ADH1C gene were more sensitive to the change of serum vitamin A than TT types. Furthermore, this finding has the potential to enable a higher marbling score under the condition of vitamin A restriction in Korean native steers.

Keywords

Vitamin A Restriction;Alcohol Dehydrogenase 1C (ADH1C);Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP);Marbling;Korean Native Steers

Acknowledgement

Supported by : Rural Development Administration

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