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Anti-cancer and anti-inflammatory effects of convergence of ginsenoside Rh2, compound K isolated from amplified red ginseng

증폭시킨 홍삼으로부터 분리한 ginsenoside Rh2, compound K의 융복합적 항암 및 항염효과

  • Kim, Young-Ho (Dept. of Naturopathic, Dongbang Culture University) ;
  • Kim, Jong-Du (Dept. of Naturopathic, Dongbang Culture University)
  • 김영호 (동방문화대학원대학교 자연치유학과) ;
  • 김종두 (동방문화대학원대학교 자연치유학과)
  • Received : 2017.08.30
  • Accepted : 2017.11.20
  • Published : 2017.11.28

Abstract

This study aims to provide basic data on useful functional ingredients in red ginseng by studying the anti-inflammatory and anti-cancer effects of convergence of ginsenoside Rh2(Rh2) and compound K(CK) isolated from amplified red ginseng. Therefore we examined cytotoxicity in Hep3B, activity of IL-6 induced STAT3 luciferase and survival concentration of cells in B16F10 and HaCa T. According to the experimental results, when the Rh2 and CK mixture were 10 ug/ml, there was no cytotoxicity in Hep3B cells and the anti-inflammatory effect of IL-6 reduction ratio was 102%. In addition, Rh2 and CK mixture were observed to be toxic in melanoma cell line B16F10 and HaCa T (human keratinocyte) at 50 uM. FACS(fluorescence activated cell sorting) analysis showed that annexin V was not expressed and melanoma cells and keratinocyte were desorbed and killed. It can be assumed that the mechanism of killing through this phenomenon is due to the cell death of anoikis-type, and it is necessary to study the changes of cell adhesion proteins in the future in order to clarify the cell death signal system.

References

  1. W. C. Cho, "Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats." Eur. J. Pharmacol. Vol. 550, No. 1, pp.173-179, 2006. https://doi.org/10.1016/j.ejphar.2006.08.056
  2. J. Y. Cho, "Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation." Planta Med, Vol. 68, No. 6, pp.497-500, 2002. https://doi.org/10.1055/s-2002-32556
  3. A-Young Jang, Yun-Chul Sueng, Joong-gu Ji, "The comparative study on physiological activity of White ginseng, Red ginseng and Black ginseng extract", Journal of Digital Convergence, Vol.14 No.5 pp.459-471, 2016. https://doi.org/10.14400/JDC.2016.14.5.459
  4. Jong Rok Lee, Young Woo Kim , Sung Hui Byun, Sang Chan Kim, Sook Jahr Park, "Anti-inflammatory effects of the fermentation extracts consisting of soybean, red ginseng and Citrus Unshiu Peel", The Korea journal of herbology, Vol.30 No.5, pp.59-65, 2015. https://doi.org/10.6116/KJH.2015.30.5.59.
  5. Byung-Moon Jin1, Min-Kyung Lee, Jun-Seon Lee, Kyung-Yae Hyun, "Anti-inflammatory Effects of Korean red ginseng Extract in formalin-induced Orofacial Pain in Rats", Journal of the Korea Academia-Industrial cooperation Society, Vol.15 No.9, pp.5708-5715, 2014. https://doi.org/10.5762/KAIS.2014.15.9.5708
  6. Seong-Hwa Oh, Soo-Yeon Choi, Nu Rim Lee, Jung No Lee, Dong-Seok Kim, Sang-Hwa Lee, and Sung-Min Park, "Cell migration and Anti-inflammatory Effect of Red Ginseng Extracts Fermented with Laetiporus Sulphureus", Journal of the society of cosmetic scientists of Korea, Vol.40 No.3, pp. 297-305, 2014. https://doi.org/10.15230/SCSK.2014.40.3.297
  7. Ji Su Shin, Jong-Myoung Kim1, Won Gun An, "Anti-inflammatory Effect of Red Ginseng through Regulation of MAPK in Lipopolysaccharidestimulated RAW264.7", Journal of physiology & pathology in Korean Medicine, Vol.26, No.3, pp. 293-300, 2012.
  8. L. P. Christensen, "Ginsenosides: Chemistry, Biosynthesis, Analysis, and Potential Health Effects. Advances in Food and Nutrition Research." Vol.55, pp.1-99, 2008.
  9. Chang-Eui Hong, Su-Yun Lyu, "Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes", Immune network : official journal of the Korean association of immunobiologists, Vol.11 No.1, pp. 42-49, 2011. https://doi.org/10.4110/in.2011.11.1.42
  10. Muhammad Jahangir Hossen, Yong Deog Hong, Kwang-Soo Baek, Sulgi Yoo, Yo Han Hong, Ji Hye Kim, Jeong-Oog Lee, Donghyun Kim, Junseong Park, Jae Youl Cho, "In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng", Journal of ginseng research, Vol.41 No.1, pp. 43-51, 2017. https://doi.org/10.1016/j.jgr.2015.12.009
  11. S. Jung, M.S. Lee, Y. Shin, C.T .Kim, I.H. Kim, Y.S. Kim, Y. Kim, "Anti-obesity and anti-inflammatory effects of high hydrostatic pressure extracts of ginseng in high-fat diet induced obese rats", Journal of functional foods : the official journal of the International Society for Nutraceuticals & Functional Foods, Vol.10 , pp. 169-177, 2014
  12. M Oh, Y H Choi, S Choi, H Chung, K Kim, S I Kim, D K Kim, N D Kim, "Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells", International journal of oncology, Vol.14, No.5, pp. 869-875, 1999.
  13. Ae-Jung Kim, Myung-Rhun Han, Soo-Jeong Lee, "Antioxidative Capacity and Quality Characteristics of Yanggaeng using Fermented Red Ginseng for the Elderly", The Korean journal of food and nutrition, Vol.25, No.1, pp. 83-89, 2012. https://doi.org/10.9799/ksfan.2012.25.1.083
  14. B. M. Kong, "Physico-Chemical Characteristics of White, Fermented and Red Ginseng Extracts." J. Ginseng Res. Vol. 32, No. 3 pp.238-243, 2008. https://doi.org/10.5142/JGR.2008.32.3.238
  15. Hee Kang, Yun-Gu Hwang, Taek-Guen Lee, Cheng-R Jin, Chi Heung Cho, Hee-Yeong Jeong, Dae-Ok Kim, "Use of Gold Nanoparticle Fertilizer Enhances the Ginsenoside Contents and Anti-Inflammatory Effects of Red Ginseng", Journal of microbiology and biotechnology, Vol.26, No.10, pp. 1668-1674, 2016. https://doi.org/10.4014/jmb.1604.04034
  16. Hyun-Joo Jung, Hojin Choi, Hye-Won Lim, Daehyun Shin, Hacksoo Kim, Bin Kwon, Jong Eun Lee, Eun-Hee Park, Chang-Jin Lim, "Enhancement of anti-inflammatory and antinociceptive actions of red ginseng extract by fermentation", Journal of pharmacy and pharmacology, Vol.64, No.5, pp. 756-762, 2012. https://doi.org/10.1111/j.2042-7158.2012.01460.x
  17. Hye-Jin Park, Da-Hye Jung, Haemi Joo, Nam-Sung Kang, Seon-A Jang, Jae Geun Lee, and Eun-Hwa Sohn, "The comparative study of anti-allergic and anti-inflammatory effects by fermented red ginseng and red ginseng" , Korean journal of plant resources, Vol.23, No.5, pp. 415-422, 2010.
  18. Byung-Weon Kim, Yoon-Bok Lee, Jae-Seung Park, Ji-Won Park, Seock-Yeon Hwang, "The Effect of Red Ginseng Extracts on Blood Biochemical Parameters, Bone Density and Bone Inorganic Components in Ovariectomized Rats", The Journal of digital policy & management, Vol.11, No.10, pp. 493-505, 2013.
  19. Byung-Weon Kim, Yoon-Bok Lee, Jae-Seung Park, Ji-Won Park, Seock-Yeon Hwang, "Effect of Red Ginseng Saponin Fraction on the Blood Components of Male Guinea Pigs with Acute Toxicity induced by 2,3,7,8-Tetracholorodibenzo-${\rho}$-dioxin (TCDD)" , The Journal of digital policy & management, Vol.11, No.4, pp. 339-350, 2013.
  20. K. Y. Nam, "The comparative understanding between red ginseng and white ginsengs, processed ginsengs(panax ginseng C. A. Meyer)." J. Ginseng Res, Vol. 29, No. 1, pp.1-18, 2005. https://doi.org/10.5142/JGR.2005.29.1.001
  21. H. Sung, "Beneficial effects of a combination of Korean red ginseng and highly active antiretroviral therapy in human immunodeficiency virus type 1-infected patients." Clin. Vaccine Immunol. Vol. 16, No. 8, pp.1127-1131, 2009. https://doi.org/10.1128/CVI.00013-09
  22. E. A. Bae, "antiallergic and antipsoriatic effects of korean red ginseng." J. Ginseng Res, Vol. 29, No. 2, pp.80-85, 2005. https://doi.org/10.5142/JGR.2005.29.2.080
  23. D. J. Jang, "Red ginseng for treating erectile dysfunction: a systematic review." Br. J. Clin. Pharmacol, Vol. 66, No. 4, pp.444-450, 2008. https://doi.org/10.1111/j.1365-2125.2008.03236.x
  24. Jongsun Park, Jae Youl Cho, "Anti-inflammatory effects of ginsenosides from Panax ginseng and their structural analogs", African journal of biotechnology, Vol.8, No.16, pp. 3682-3690, 2009
  25. Frisch SM, Screaton RA, "Anoikis mechanisms", Current Opinion in Cell Biology, Vol.13, No.5, pp.555-562,2001, http://dx.doi.org/10.1016/S0955-0674(00)00251-9 https://doi.org/10.1016/S0955-0674(00)00251-9