Immune-enhancing effects of polysaccharides with different molecular weights obtained from Gloiopeltis furcata

불등풀가사리 다당류의 분자량에 따른 면역증강 효과

  • Lee, Dae-Hoon (Department of Food Science and Technology, Daegu Catholic University) ;
  • Hong, Joo-Heon (Department of Food Science and Technology, Daegu Catholic University)
  • 이대훈 (대구가톨릭대학교 식품공학전공) ;
  • 홍주헌 (대구가톨릭대학교 식품공학전공)
  • Received : 2018.01.17
  • Accepted : 2018.02.23
  • Published : 2018.02.28


This study investigated the immune-enhancing effects of polysaccharides extracted from Gloiopeltis furcata (red seaweed) with different molecular weights. A crude polysaccharide mixture was hydrolyzed using acid treatment (0.1 N HCl) and three molecular weight fractions were generated and filtered using centrifugation: (${\leq}10kDa$, 10 to 100 kDa, and 100 kDa. Nitric oxide (NO) production in RAW264.7 cells treated with $0.01-0.5{\mu}g/mL$ polysaccharides ${\geq}100kDa$ was $12.28-19.05{\mu}M$. Treatment with polysaccharides ${\geq}100kDa$ increased cytokine levels, including TNF-${\alpha}$ and IL-6 levels, in a dose-dependent manner. Polymerase chain reaction analysis also revealed marked increases in iNOS and COX-2 mRNA expression levels. These findings lead us to conclude that macrophage activation induced by polysaccharides ${\geq}100kDa$ was greater than that induced by polysaccharides ${\leq}10kDa$ or between 10 and 100 kDa. The polysaccharides ${\geq}100kDa$ extracted from Gloiopeltis furcata investigated herein are potentially useful as natural immune-enhancing agents. These findings provided further insights into the potential use of ${\geq}100kDa$ as immunopotentiator or new function food.


  1. Yu KX, Jantan I, Ahmad R, Wong CL (2014) The major bioactive components of seaweeds and their mosquitocidal potential. Parasitol Res, 113, 3121-3141
  2. Cheng YL, Juang YC, Liao GY, Ho SH, Yeh KL, Chen CY, Chang JS, Liu JC, Lee DJ (2010) Dispersed ozone flotation of Chlorella vulgaris. Bioresour Technol, 101, 9092-9096
  3. Hultberg M, Carlsson AS, Gustafsson S (2013) Treatment of drainage solution from hydroponic greenhouse production with microalgae. Bioresour Technol, 136, 401-406
  4. Nader HB, Lopes CC, Rocha HA, Santos EA, Dietrich CP (2004) Heparins and heparinoids: occurrence, structure and mechanism of antithrombotic and hemorrhagic activities. Curr Pharm Des, 10, 951-966
  5. Lahaye M, Robic A (2007) Structure and functional properties of ulvan, a polysaccharide from green seaweeds. Biomacromolecules, 8, 1765-1774
  6. Mao W, Zang X, Li Y, Zhang H (2006) Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity. J Appl phycol, 18, 9-14
  7. Fang Z, Jeong SY, Jung HA, Choi JS, Min BS, Woo MH (2010) Anticholinesterase and antioxidant constituents from Gloiopeltis furcata. Chem Pharm Bull, 58, 1236-1239
  8. Niu R, Fan X, Han L (2003) A screening for the anti-inflammatory effect of algal extracts. Oceanol Limnol Sin, 34, 150-154
  9. Bae SJ, Choi YH (2007) Methanol extract of the seaweed Gloiopeltis furcata induces G2/M arrest and inhibits cyclooxygenase 2 activity in human hepatocarcinoma HepG2 cells. Phytother Res, 21, 52-57
  10. Zhang Q, Yu P, Li Z, Zhang H, Xu Z, Li P (2003) Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis. J Appl phycol, 15, 305-310
  11. Zhou G, Sun Y, Xin H, Zhang Y, Li Z, Xu Z (2004) In vivo antitumor and immunomodulation activities of different molecular weight lambda-carrageenans from Chondrus ocellatus. Pharmacol Res, 50, 47-53
  12. Qi H, Zhang Q, Zhao T, Chen R, Zhang H, Niu X, Li Z (2005) Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int J Biol Macromol, 37, 195-199
  13. Lee DH, Hong JH (2017) Physicochemical properties and anti-wrinkle effect of polysaccharides with different molecular weights from Gloiopeltis furcata. Korean J Food Preserv, 24, 688-696
  14. Li JE, Nie SP, Xie MY, Li C (2014) Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities. J Funct Foods, 6, 410-418
  15. Kim JK, Cho ML, Karnjanapratum S, Shin IS, You SG (2011) In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from Enteromorpha prolifera. Int J Biol Macromol, 49, 1051-1058
  16. Nakagawa T, Yokozawa T (2002) Direct scavenging of nitric oxide and superoxide by green tea. Food Chem Toxicol, 40, 1745-1750
  17. Leiro JM, Castro R, Arranz JA, Lamas J (2007) Immunomodulating activities of acidic sulphated polysaccharides obtained from the seaweed Ulva rigida C. Agardh. Int Immunopharmacol, 7, 879-888
  18. Tabarsa M, You S, Dabaghian EH, Surayot U (2017) Water-soluble polysaccharides from Ulva intestinalis: Molecular properties, structural elucidation and immunomodulatory activities. J Food Drug Anal, 30, 1-10
  19. Peters M, zum Buschenfelde KHM, Rose-John S (1996) The function of the soluble IL-6 receptor in vivo. Immunol Lett, 54, 177-184
  20. Furusawa E, Furusawa S (1985) Anticancer activity of a natural product, viva-natural, extracted from Undaria pinnantifida on intraperitoneally implanted Lewis lung carcinoma. Oncol, 42, 364-369
  21. Gill N, Davies EJ, Ashkar AA (2008) The role of tolllike receptor ligands/agonists in protection against genital HSV 2 infection. Am J Reprod Immunol, 59, 35-43
  22. Choi EM, Kim AJ, Kim YO, Hwang JK (2005) Immunomodulating activity of arabinogalactan and fucoidan in vitro. J Med Food, 8, 446-453
  23. Lee JB, Ohta Y, Hayashi K, Hayashi T (2010) Immunostimulating effects of a sulfated galactan from Codium fragile. Carbohydr Res, 345, 1452-1454