Antioxidant activities and anti-inflammatory effects of fresh and air-dried Abeliophyllum distichum Nakai leaves

건조방법에 따른 미선나무 잎의 항산화 및 항염증 효과

  • Chang, Seong Jun (Teakyung food and processing R&D center) ;
  • Jeon, Nam Bae (Teakyung food and processing R&D center) ;
  • Park, Joo Won (Teakyung food and processing R&D center) ;
  • Jang, Tae Won (Department of Medicinal Plant Resources, Andong National University) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)
  • 장성준 (태경농산(주) 연구소) ;
  • 전남배 (태경농산(주) 연구소) ;
  • 박주원 (태경농산(주) 연구소) ;
  • 장태원 (안동대학교 생약자원학과) ;
  • 정진부 (안동대학교 생약자원학과) ;
  • 박재호 (중원대학교 생약자원개발학과)
  • Received : 2017.12.06
  • Accepted : 2018.01.26
  • Published : 2018.02.28


In this study, we evaluated the antioxidant activity and anti-inflammatory effects of Abeliophyllum distichum (A. distichum) leaves that were prepared via air-drying. Fresh and air-dried A. distichum leaves were examined via 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay and measurements of the reducing power. The suppression effects on inflammation of the leaves were analyzed by a western blot and RT-PCR on LPS-induced RAW 264.7 cells. As a result, the antioxidant activity of the fresh leaves was found to be more effective than that of the air-dried leaves. Also, the fresh leaves were more effective in suppressing the protein and mRNA levels of iNOS and COX-2 than the air-dried leaves, thereby indicating the better anti-inflammatory effects. In addition, the contents of phenolic compounds and acteoside were analyzed by high-performance liquid chromatography (HPLC). The results showed that the acteoside content decreased with the use of the air-drying method, while there was no change in the content of phenolic compounds. Therefore, this study indicated that fresh A. distichum leaves potential antioxidant and suppression activities of various factors that are involved in the production of NO, which were found to be better than those of air-dried A. distichum leaves. These biological activities were also found to be independent of the content of phonolic compounds and were assumed to be directly or indirectly related to the content of acteoside.


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