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Anti-inflammation effect of blueberry (Vaccinium ashei) leaf extract on RAW 264.7 macrophages stimulated by lipopolysaccharide

Lipopolysaccharide에 의해 활성화된 RAW 264.7대식세포에서 블루베리 잎(Vaccinium ashei) 추출물의 항염증 효과

  • Kim, Dong In (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Kim, Hyun Jung (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Yun, Jong Moon (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Lee, Ji Hye (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Han, So Jung (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Kim, Ha Eun (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Jang, Min Jung (R&D Team, Humancosmetic Co., Ltd.) ;
  • An, Bong Jeun (Department of Cosmeceutical sciece, DaeguHanny University)
  • 김동인 (대구한의대학교 화장품약리학과) ;
  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 윤종문 (대구한의대학교 화장품약리학과) ;
  • 이지혜 (대구한의대학교 화장품약리학과) ;
  • 한소정 (대구한의대학교 화장품약리학과) ;
  • 김하은 (대구한의대학교 화장품약리학과) ;
  • 장민정 (휴먼코스메틱(주)) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2018.01.11
  • Accepted : 2018.02.19
  • Published : 2018.02.28

Abstract

The aim of this study is to investigate the antioxidant and intracellular anti-inflammatory efficacy of blueberry leaf extracted with hot water (BLW), 70% ethanol (BLE), and 70% acetone (BLA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of blueberry leaf extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measure by Western blotting and real-time PCR methods. i-NOS, COX-2 protein, and mRNA expression showed concentration-dependent decrease. The decreases in the mRNA expression levels of interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and prostaglandin $E_2$ ($PGE_2$) were concentration-dependent. Further, the antioxidant effects of blueberry leaf on total polyphenol contents, electron donating ability and $ABTS^+$ radical scavenging activity were evaluated. The total polyphenol contents of BLW, BLE, and BLA were $217.04{\pm}2.98$, $156.72{\pm}3.90$, and $182.88{\pm}3.02mg\;TAE/g$, respectively, while the electron donating abilities at $1,000{\mu}g/mL$ of BLW, BLE, and BLA were 81.7, 79.6, and 79.3%, respectively. The $ABTS^+$ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at $50{\mu}g/mL$ of BLW, BLE, and BLA were 35.1, 42.4 and 42.7%, respectively. In conclusion, the antioxidant and anti-inflammatory test results indicate that blueberry leaf extracts (BLW, BLE, and BLA) can be used as potential anti-inflammatory agents.

Acknowledgement

Supported by : 산업통상자원부

References

  1. Haddad JJ (2002) Antioxidnat and prooxidant mechanisms in the regulation of redox (y)-sensitive transcription factors. Cell signalling, 14, 879-897
  2. Beckman KB, ames BN (1998) The free radical theory of aging matures. Physiol Rev, 78, 547-581
  3. Ding C, Cicuttini F, Li J, Jones G (2009) Targeting IL-6 in the treatment of inflammatory and autoimmune diseases. Expert Opin Investig Drugs, 18, 1457-1466
  4. Rankin JA (2004) Biological mediators of acute inflammation. AACN Clin Issues, 15, 3-17
  5. Guzik TJ, Korbut R, Adamek-Guzik T (2003) Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol, 54, 469-487
  6. Goodwin JS, Ceuppens J (1983) Regulation of the immune response by prostaglandins. J Clin Immunol, 3, 295-315
  7. Li X, Xu X (2011) TLR4-mediated activation of macrophages by the polysaccharide fraction from Polyporus umbellatus (pers.) Fries. J Ethnopharmacol, 135, 1-6
  8. Sims JE, Gayle MA, Slack JL, Alderson MR, Bird TA, Giri JG, Colotta F, Re F, Mantovani A, Shanebeck K, Grabstein KH, Dower SK (1993) Interleukin 1 signaling occurs exclusively via the type I receptor. Proc Natl Acad Sci USA, 90, 6155-6159
  9. Grossman RM, Krueger J, Yourish D, Granelli-Piperno A, Murphy DP, May LT, Kupper TS, Sehgal PB, Gottlieb AB (1989) Interleukin 6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes. Proc Natl Acad Sci USA, 86, 6367-6371
  10. Westwood MN (1993) Temperate-zone pornology. Timeber press, Portland, OR, USA, p 100-101
  11. Jeong HR, Jo YN, Jeong JH, Kim HJ, Heo HJ (2012) Nutritional composition and in vitro antioxidant activities of blueberry (Vaccinium ashei) leaf. Korean J Food Preserv, 19, 604-610
  12. Jeong HR (2011) Anti-amnesic effects of blueberry leaf extracts amyloid $\beta$ protein-induced alzheimer's disease model. MS Thesis, Gyungsang National University, Korea, p 1-65
  13. Kim MK, Lee IC (2014) Anti-oxidant Anti-inflammatory Effects of Extracts from Blueberry (Vaccinium ashei) Leaf. J Korea Soc Beauty Art, 15, 109-120
  14. Matsuo Y, Fujita Y, Ohnishi S, Tanaka T, Hirabaru H, Kai T, Sakaida H, Nishizono S, Kouno I (2010) Chemical constituents of the leaves of rabbiteye blueberry (Vaccinium ashei) and characterisation of polymeric proanthocyanidins containing phenylpropanoid units and A-type linkages. Food Chem, 121, 1073-1079
  15. Folin O, Denis W (1912) On phosphotungastic phosphomolybdic compounds as color regents. J Biol Chem, 12, 239-249
  16. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med, 26, 1231-1237
  18. Stirpe F, Della Corte E (1969) The regulation of rat liver xanthine oxidase: conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem, 244, 3855-3863
  19. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res, 47, 936-942
  20. Bartholomew B (1984) A rapid method for the assay of nitrate in urine using the nitrate reductase enzyme of Escherichia coli. Food Chem Toxicol, 22, 541-543
  21. Duthie G, Crozier A (2000) Plant-derived phenolic antioxidants. Curr Opin Clin Nutr Metab Care, 3, 447-451
  22. Ferreres F, Gomes D, Valentao P, Goncalves R, Pio R, Chagas EA, Seabra RM, Andrade PB (2009) Improved loquat (Eriobotrya japonica Lindl.) cultivars: variation of phenolics and antioxidative potential. Food Chem, 114, 1019-1027
  23. Shahidi F, Janitha PK, Wanasundara PD (1992) Phenolic antioxidants. Crit Rev Food Sci and Nutr, 32, 67-103
  24. Jeong CH, Kang ST, Joo OS, Lee SC, Shin YH, Shim KH, Cho SH, Choi SG, Heo HJ (2009) Phenolic content, antioxidant effect and acetylcholinesterase inhibitory activity of korea commercial green, puer, oolong, and black teas. Korean J Food Preserv, 16, 230-237
  25. Aoshima H, Tsunoue H, Koda H, Kiso Y (2004) Aging of whiskey increases 1,1-diphenyl1-2-picrylhydrazyl radical scavenging activity. J Agric Food Chem, 52, 5240-5244
  26. Samak G, Shenoy RP, Manjunatha S, Vinayak K (2009) Superoxide and hydroxyl radical scavenging actions of botanical extracts of Wagatea spicata. Food Chem, 115, 631-634
  27. Gulcin I, Berashvili D, Gepdiremen A (2005) Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. J Ethnopharmacol, 101, 287-293
  28. Gulcin I (2006) Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217, 213-220
  29. Joung YM, Park SJ, Lee KY, Lee JY, Suh JK, Hwang SY, Park KE, Kang MH (2007) Antioxidant and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Korean J Food Sci Technol, 39, 452-457