Anti-inflammation effect of blueberry (Vaccinium ashei) leaf extract on RAW 264.7 macrophages stimulated by lipopolysaccharide

Lipopolysaccharide에 의해 활성화된 RAW 264.7대식세포에서 블루베리 잎(Vaccinium ashei) 추출물의 항염증 효과

  • Kim, Dong In (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Kim, Hyun Jung (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Yun, Jong Moon (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Lee, Ji Hye (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Han, So Jung (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Kim, Ha Eun (Department of Cosmeceutical sciece, DaeguHanny University) ;
  • Jang, Min Jung (R&D Team, Humancosmetic Co., Ltd.) ;
  • An, Bong Jeun (Department of Cosmeceutical sciece, DaeguHanny University)
  • 김동인 (대구한의대학교 화장품약리학과) ;
  • 김현정 (대구한의대학교 화장품약리학과) ;
  • 윤종문 (대구한의대학교 화장품약리학과) ;
  • 이지혜 (대구한의대학교 화장품약리학과) ;
  • 한소정 (대구한의대학교 화장품약리학과) ;
  • 김하은 (대구한의대학교 화장품약리학과) ;
  • 장민정 (휴먼코스메틱(주)) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2018.01.11
  • Accepted : 2018.02.19
  • Published : 2018.02.28


The aim of this study is to investigate the antioxidant and intracellular anti-inflammatory efficacy of blueberry leaf extracted with hot water (BLW), 70% ethanol (BLE), and 70% acetone (BLA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of blueberry leaf extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measure by Western blotting and real-time PCR methods. i-NOS, COX-2 protein, and mRNA expression showed concentration-dependent decrease. The decreases in the mRNA expression levels of interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and prostaglandin $E_2$ ($PGE_2$) were concentration-dependent. Further, the antioxidant effects of blueberry leaf on total polyphenol contents, electron donating ability and $ABTS^+$ radical scavenging activity were evaluated. The total polyphenol contents of BLW, BLE, and BLA were $217.04{\pm}2.98$, $156.72{\pm}3.90$, and $182.88{\pm}3.02mg\;TAE/g$, respectively, while the electron donating abilities at $1,000{\mu}g/mL$ of BLW, BLE, and BLA were 81.7, 79.6, and 79.3%, respectively. The $ABTS^+$ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at $50{\mu}g/mL$ of BLW, BLE, and BLA were 35.1, 42.4 and 42.7%, respectively. In conclusion, the antioxidant and anti-inflammatory test results indicate that blueberry leaf extracts (BLW, BLE, and BLA) can be used as potential anti-inflammatory agents.


Supported by : 산업통상자원부


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