DOI QR코드

DOI QR Code

Effect of microbial inoculants on fermentation quality and aerobic stability of sweet potato vine silage

  • Joo, Young Ho (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Kim, Dong Hyeon (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Paradhipta, Dimas H.V. (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Lee, Hyuk Jun (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University) ;
  • Amanullah, Sardar M. (Biotechnology Division, Bangladesh Livestock Research Institute) ;
  • Kim, Sang Bum (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Chang, Jong Soo (Department of Agricultural Science, Korea National Open University) ;
  • Kim, Sam Churl (Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
  • Received : 2018.04.02
  • Accepted : 2018.06.29
  • Published : 2018.12.01

Abstract

Objective: This study was conducted to evaluate the effect of homo or hetero fermentative inoculants on fermentation quality and aerobic stability of sweet potato vine (SPV) silage containing Italian ryegrass hay as moisture absorbent. Methods: The SPV was harvested at 15% dry matter, mixed with Italian ryegrass hay at 1:1 ratio on a fresh weight basis, and chopped to 3 to 5 cm length. After then, the chopped forage mixture was ensiled into 20-L mini silos in quadruplicate for 7, 48, and 100 days after application of microbial inoculants at $1.2{\times}10^5$ colony forming units (cfu)/g of forage following: no inoculant (CON), Lactobacillus plantarum as a homo fermentative (LP), Lactobacillus buchneri as a hetero fermentative (LB), and mixture of LP and LB at 1:1 ratio as a combo fermentative (MIX). Results: The LP and MIX silages had lowest pH (p<0.001) on 7 and 48 days, while MIX and CON silages had greatest lactate concentrations (p<0.05) on 7 and 48 days, respectively. Acetate concentrations were highest (p<0.01) in LB and MIX silages on 7 days, and in LB silage on 48 days, while lactate to acetate ratios were lowest (p<0.001) in LB silages. The chemical compositions and nutrient digestibility of silage ensiled for 100 days was not affected by inoculants. On 100 days of ensiling, LB silage had lowest (p<0.01) lactate concentration and lactate to acetate ratio, but highest acetate concentration. Aerobic stability was highest (p<0.001) in LB silage followed in MIX silage. On contrast, LB silage had lowest (p<0.05) lactic acid bacteria and mold. Conclusion: The results indicated that application of LB solely had a better effect on aerobic stability than not only LP, but also MIX. However, LP application did not show beneficial effects from the viewpoints of fermentation quality and aerobic stability compared to CON.

Keywords

Aerobic Stability;Microbial Inoculant;Silage;Sweet Potato Vine

Acknowledgement

Supported by : IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries)

References

  1. Kariuki JN, Gachuiri CK, Gitau GK, et al. Effect of feeding napier grass, lucerne, and sweet potato vines as sole diets to dairy heifers on nutrient intake, weight gain, and rumen degradation. Livest Prod Sci 1998;55:13-20. https://doi.org/10.1016/S0301-6226(98)00127-4
  2. Li P, Ji S, Wang Q, et al. Adding sweet potato vines improve the quality of straw silage. Anim Sci J 2017;88:625-32. https://doi.org/10.1111/asj.12690
  3. Aregheore EM. Nutritive value of sweet potato (Ipomea batatas (L) Lam) forage as goat feed: voluntary intake, growth and digestibility of mixed rations of sweet potato and batiki grass (Ischaemum aristatum var. indicum). Small Rumin Res 2004;51:235-41. https://doi.org/10.1016/S0921-4488(03)00198-6
  4. Pedrosa CE, Andrade Júnior VC, Pereira RC, et al. Yield and quality of wilted sweet potato vines and its silages. Hortic Bras 2015;33:283-9. https://doi.org/10.1590/S0102-053620150000300002
  5. Van An L, Lindberg JE. Ensiling of sweet potato leaves (Ipomoea batatas (L.) Lam) and the nutritive value of sweet potato leaf silage for growing pigs. Asian-Australas J Anim Sci 2004;17:497-503. https://doi.org/10.5713/ajas.2004.497
  6. Nguyen THL, Ngoan LD, Bosch G, Verstegen MWA, Hendriks WH. Ileal and total tract apparent crude protein and amino acid digestibility of ensiled and dried cassava leaves and sweet potato vines in growing pigs. Anim Feed Sci Technol 2012;172:171-9. https://doi.org/10.1016/j.anifeedsci.2011.11.009
  7. Khalid AF, Elamin KM, Amin AE, et al. Effect of feeding sweet potato (Ipomoea batatas) vines silage on performance and milk production of Nubian goats. J Vet Adv 2013;3:153-9. https://doi.org/10.5455/jva.0130517045658
  8. McDonald P, Henderson AR, Heron SJE. The biochemistry of silage, 2nd ed. Bucks, UK: Chalcombe Publ.; 1991.
  9. Wang S, Yuan W, Dong Z, et al. Characteristics of isolated lactic acid bacteria and their effects on the silage quality. Asian-Australas J Anim Sci 2017;30:819-27.
  10. Kung LJr, Taylor CC, Lynch MP, Neylon JM. The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows. J Dairy Sci 2003;86:336-43. https://doi.org/10.3168/jds.S0022-0302(03)73611-X
  11. Filya I. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. J Dairy Sci 2003;86:3575-81. https://doi.org/10.3168/jds.S0022-0302(03)73963-0
  12. AOAC. Official methods of analysis. 15th edn, Association of Official Analytical Chemists, Arlington, VA, USA: AOAC International; 1995.
  13. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, natural detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  14. Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crops. Grassl Forage Sci 1963;18:104-11. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  15. Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem 1962;8:130-2.
  16. Muck RE, Dickerson JT. Storage temperature effects on proteolysis in alfalfa silage. Trans ASASE 1988;3:1005-9.
  17. Arriola KG, Kim SC, Staples CR, Adesogan AT. Effect of applying bacterial inoculants containing different types of bacteria to corn silage on the performance of dairy cattle. J Dairy Sci 2011;94:3973-9. https://doi.org/10.3168/jds.2010-4070
  18. SAS Institute Inc. SAS/STAT user's guide: Version 9. Cary, NC, USA: SAS Institute Inc.; 2002.
  19. Elferink SJWH, Krooneman J, Gottschal JC, et al. Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri. Appl Environ Microbiol 2001;67:125-32. https://doi.org/10.1128/AEM.67.1.125-132.2001
  20. Driehuis F, Oude Elferink SJWH, Wikselaar PGV. Fermentation characteristic and aerobic stability of grass silage inoculated with Lactobacillus buchneri, with or without homofermentative lactic acid bacteria. Grass Forage Sci 2001;56:330-43. https://doi.org/10.1046/j.1365-2494.2001.00282.x
  21. Rooke JA, Hatfield RD. Biochemistry of ensiling. In: Buxton DR, Muck RE and Harrison HJ, editors. Silage science and technology (Agronomy Series No. 42). Madison, WI, USA: American Society of Agronomy; 2003. p. 95-139.
  22. Queiroz OCM, Adesogan AT, Arriola KG, Queiroz MFS. Effect of a dual-purpose inoculant on the quality and nutrient losses from corn silage produced in far-scale silos. J Dairy Sci 2012;95:3354-62. https://doi.org/10.3168/jds.2011-5207
  23. Mangwe MC, Rangubhet KT, Mlambo V, Yu B, Chiang HI. Effects of Lactobacillus formoensis $S215^{T}$ and Lactobacillus buchneri on quality and in vitro ruminal biological activity of condensed tannins in sweet potato vines silage. J Appl Microbiol 2016;121:1242-53. https://doi.org/10.1111/jam.13260
  24. Weinberg ZG, Shatz O, Chen Y, et al. Effect of lactic acid bacteria inoculants on in vitro digestibility of wheat and corn silages. J Dairy Sci 2007;90:4754-62. https://doi.org/10.3168/jds.2007-0176
  25. Weinberg ZG, Ashbell G, Hen Y. The effect of Lactobacillus buchneri and L. plantarum, applied at ensiling, on the ensiling fermentation and aerobic stability of wheat and sorghum silages. J Ind Microbiol Biotechnol 1999;23:218-22. https://doi.org/10.1038/sj.jim.2900726
  26. Kim DH, Amanullah SM, Lee HJ, Joo YH, Kim SC. Effect of microbial and chemical combo additives on nutritive value and fermentation characteristics of whole crop barley silage. Asian-Australas J Anim Sci 2015;28:1274-80. https://doi.org/10.5713/ajas.15.0106
  27. Danner H, Holzer M, Mayrhuber E, Braun R. Acetic acid increases stability of silage under aerobic condition. Appl Environ Microbiol 2003;69:562-7. https://doi.org/10.1128/AEM.69.1.562-567.2003
  28. O'Kiely P, Muck RE. Aerobic deterioration of Lucerne (Mediacago sativa) and maize (Zea mays) silage-effects of yeasts. J Sci Food Agric 1992;67:123-32.
  29. Li Y, Wang F, Nishino N. Lactic acid bacteria in total mixed ration silage containing soybean curd residue: Their isolation, identification and ability to inhibit aerobic deterioration. Asian-Australas J Anim Sci 2016;29:516-22. https://doi.org/10.5713/ajas.15.0267
  30. Dalie DKD, Deschamps AM, Richard-Forget F. Lactic acid bacteria-Potential for control of mold growth and mycotoxins: A reviw. Food Control 2010;21:370-80. https://doi.org/10.1016/j.foodcont.2009.07.011