Effect of inoculants and storage temperature on the microbial, chemical and mycotoxin composition of corn silage

  • Wang, Musen (Department of Grassland Science, China Agricultural University) ;
  • Xu, Shengyang (Department of Grassland Science, China Agricultural University) ;
  • Wang, Tianzheng (Department of Grassland Science, China Agricultural University) ;
  • Jia, Tingting (Department of Grassland Science, China Agricultural University) ;
  • Xu, Zhenzhen (Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science) ;
  • Wang, Xue (Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science) ;
  • Yu, Zhu (Department of Grassland Science, China Agricultural University)
  • Received : 2017.11.03
  • Accepted : 2018.01.26
  • Published : 2018.12.01


Objective: To evaluate the effect of lactic acid bacteria and storage temperature on the microbial, chemical and mycotoxin composition of corn silage. Methods: Corn was harvested at 32.8% dry matter, and chopped to 1 to 2 cm. The chopped material was subjected to three treatments: i) control (distilled water); ii) $1{\times}10^6$ colony forming units (cfu)/g of Lactobacillus plantarum; iii) $1{\times}10^6cfu/g$ of Pediococcus pentosaceus. Treatments in triplicate were ensiled for 55 d at $20^{\circ}C$, $28^{\circ}C$, and $37^{\circ}C$ in 1-L polythene jars following packing to a density of approximately $800kg/m^3$ of fresh matter, respectively. At silo opening, microbial populations, fermentation characteristics, nutritive value and mycotoxins of corn silage were determined. Results: L. plantarum significantly increased yeast number, water soluble carbohydrates, nitrate and deoxynivalenol content, and significantly decreased the ammonia N value in corn silage compared with the control (p<0.05). P. pentosaceus significantly increased lactic acid bacteria and yeast number and content of deoxynivalenol, nivalenol, T-2 toxin and zearalenone, while decreasing mold population and content of nitrate and 3-acetyl-deoxynivalneol in corn silage when stored at $20^{\circ}C$ compared to the control (p<0.05). Storage temperature had a significant effect on deoxynivalenol, nivalenol, ochratoxin A, and zearalenone level in corn silage (p<0.05). Conclusion: Lactobacillus plantarum and Pediococcus pentosaceus did not decrease the contents of mycotoxins or nitrate in corn silage stored at three temperatures.



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