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Effects of dietary energy and lipase levels on nutrient digestibility, digestive physiology and noxious gas emission in weaning pigs

  • Liu, J.B. (School of Life Science and Engineering, Southwest University of Science and Technology) ;
  • Cao, S.C. (School of Life Science and Engineering, Southwest University of Science and Technology) ;
  • Liu, J. (School of Life Science and Engineering, Southwest University of Science and Technology) ;
  • Pu, J. (School of Life Science and Engineering, Southwest University of Science and Technology) ;
  • Chen, L. (State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) ;
  • Zhang, H.F. (State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences)
  • Received : 2018.01.26
  • Accepted : 2018.05.10
  • Published : 2018.12.01

Abstract

Objective: This study was conducted to evaluate the effect of dietary energy and lipase supplementation on growth performance, nutrient digestibility, serum profiles, intestinal morphology, small intestinal digestive enzyme activities, biochemical index of intestinal development and noxious gas emission in weaning pigs. Methods: A total of 240 weaning pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc) with an average body weight (BW) of $7.3{\pm}0.12kg$ were used in this 28-d experiment. Weaning pigs were randomly allocated to 4 dietary treatments in a $2{\times}2$ factorial arrangement with 2 levels of energy (net energy = 2,470 kcal/kg for low energy diet and 2,545 kcal/kg for basal diet) and 2 levels of lipase (0 and 1.5 U/g of lipase) according to BW and sex. There were 6 replications (pens) per treatment and 10 pigs per pen (5 barrows and 5 gilts). Results: Weaning pigs fed the low energy diet had lower (p<0.05) gain-to-feed ratio (G:F) throughout the experiment, apparent digestibility of dry matter, nitrogen, ether extract, and gross energy during d 0 to 14, average daily gain during d 15 to 28, lipase activity in duodenum and ileum and protein/DNA in jejunum (p<0.05), respectively. Lipase supplementation had no effect on growth performance but affected apparent nutrient digestibility (p<0.05) on d 14 and enhanced lipase activity in the duodenum and ileum and protease activity in duodenum and jejunum of pigs (p<0.05) fed the low energy diet. Lipase reduced serum low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG), $NH_3$ production (p<0.05) from the feces. Conclusion: The low energy diet decreased G:F throughout the experiment and nutrient digestibility during d 0 to 14 as well as lipase activity in duodenum and ileum. Lipase supplementation increased nutrient digestibility during d 0 to 14 and exerted beneficial effects on lipase activity in duodenum and ileum as well as protease activity in duodenum and jejunum, while reduced serum LDL-C, TG and fecal $NH_3$.

Keywords

Digestibility;Enzyme Activity;Growth Performance;Lipase;Weaning Pigs

References

  1. Zhao PY, Li HL, Hossain MM, Kim IH. Effect of emulsifier (lysophospholipids) on growth performance, nutrient digestibility and blood profile in weanling pigs. Anim Feed Sci Technol 2015;207:190-5. https://doi.org/10.1016/j.anifeedsci.2015.06.007
  2. Cera KR, Mahan DC, Reinhart GA. Apparent fat digestibilities and performance responses of postweaning swine fed diets supplemented with coconut oil, corn oil or tallow. J Anim Sci 1989;67:2040-7. https://doi.org/10.2527/jas1989.6782040x
  3. Leonard SG, Sweeney T, Bahar B, Lynch BP, O'Doherty JV. Effects of dietary seaweed extract supplementation in sows and post-weaned pigs on performance, intestinal morphology, intestinal microflora and immune status. Br J Nutr 2011;106:688-99. https://doi.org/10.1017/S0007114511000997
  4. Azain MJ. Fat in swine nutrition. In: Lewis AJ, Southern LL, editors. Swine nutrition. Boca Raton, FL, USA: CRC Press; 2001. p. 95-105.
  5. Tokach MD, Pettigrew JE, Johnston LJ, et al. Effect of adding fat and (or) milk products to the weanling pig diet on performance in the nursery and subsequent grow-finish stages. J Anim Sci 1995;73:3358-68. https://doi.org/10.2527/1995.73113358x
  6. Adeola O, Cowieson AJ. Board-invited review: opportunities and challenges in using exogenous enzymes to improve nonruminant animal production. J Anim Sci 2011;89:3189-218. https://doi.org/10.2527/jas.2010-3715
  7. Dierick NA, Decuypere JA, Molly K, Van Beek E, Vanderbeke E. The combined use of triacylglycerols (TAGs) containing medium chain fatty acids (MCFAs) and exogenous lipolytic enzymes as an alternative to nutritional antibiotics in piglet nutrition. Livest Prod Sci 2002;76:1-16. https://doi.org/10.1016/S0301-6226(01)00331-1
  8. Zhang LN, Chen DW, Yu B, et al. Effects of lipase supplementation in different energy level diets on growth performance, nutrient apparent digestibility and serum biochemical indexes of weaned pigs. Chin J Anim Nutr 2015;27:3854-60.
  9. Francesch M, Geraert PA. Enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybeanbased diets. Poult Sci 2009;88:1915-24. https://doi.org/10.3382/ps.2009-00073
  10. Zhou Y, Jiang Z, Lv D, Wang T. Improved energy-utilizing efficiency by enzyme preparation supplement in broiler diets with different metabolizable energy levels. Poult Sci 2009;88:316-22. https://doi.org/10.3382/ps.2008-00231
  11. National Research Council. Nutrient requirements of swine. 11th ed. Washington, DC, USA: National Academy Press; 2012.
  12. Ball RO, Aherne FX. Effect of diet complexity and feed restriction on the incidence and severity of diarrhea in early-weaned pigs. Can J Anim Sci 1982;62:907-13. https://doi.org/10.4141/cjas82-109
  13. Song M, Liu Y, Soares JA, et al. Dietary clays alleviate diarrhea of weaned pigs. J Anim Sci 2012;90:345-60. https://doi.org/10.2527/jas.2010-3662
  14. AOAC International. Official methods of analysis of AOAC international. 18th ed. Gaithersburg, MD, USA: AOAC International; 2007.
  15. Lu P, Li D, Yin J, Zhang L, Wang Z. Flavour differences of cooked longissimus muscle from Chinese indigenous pig breeds and hybrid pig breed ($Duroc{\times}Landrace{\times}Large\;White$). Food Chem 2008;107:1529-37. https://doi.org/10.1016/j.foodchem.2007.10.010
  16. Williams CH, David DJ, Iismaa O. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. J Agric Sci 1962;59:381-5. https://doi.org/10.1017/S002185960001546X
  17. Kong C, Adeola O. Evaluation of amino acid and energy utilization in feedstuff for swine and poultry diets. Asian-Australas J Anim Sci 2014;27:917-25. https://doi.org/10.5713/ajas.2014.r.02
  18. Brown DC, Maxwell CV, Erf GF, et al. The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post-weaning pigs. Vet Immunol Immunopathol 2006;111:187-98. https://doi.org/10.1016/j.vetimm.2005.12.006
  19. Nabuurs MJA, Hoogendoorn A, Van Der Molen EJ, Van Osta ALM. Villus height and crypt depth in weaned and unweaned pigs, reared under various circumstances in the Netherlands. Res Vet Sci 1993;55:78-84. https://doi.org/10.1016/0034-5288(93)90038-H
  20. Hu C, Song J, You Z, Luan Z, Li W. Zinc oxide-montmorillonite hybrid influences diarrhea, intestinal mucosal integrity, and digestive enzyme activity in weaned pigs. Biol Trace Elem Res 2012;149:190-6. https://doi.org/10.1007/s12011-012-9422-9
  21. Somogyi M. Modifications of two methods for the assay of amylase. Clin Chem 1960;6:23-35.
  22. Tietz NW, Fiereck EA. A specific method for serum lipase determination. Clin Chim Acta 1966;13:352-8. https://doi.org/10.1016/0009-8981(66)90215-4
  23. Lemmens JWTJ. Nucleic acid levels, cellular activity and growth during the puerulus stage of the Western Rock Lobster (Panulirus cygnus (George); Decapoda: Palinuridae). J Exp Mar Biol Ecol 1995;194:143-56. https://doi.org/10.1016/0022-0981(95)00087-9
  24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. https://doi.org/10.1016/0003-2697(76)90527-3
  25. Zhang ZF, Lee JM, Kim IH. Effects of Enterococcus faecium DSM 7134 on weanling pigs were influenced by dietary energy and crude protein density. Livest Sci 2014;169:106-11. https://doi.org/10.1016/j.livsci.2014.09.022
  26. SAS Institute. SAS user's guide: statistics. Version 9.0. Cary, NC, USA: SAS Institute, Inc; 2003.
  27. Cera KR, Mahan DC, Reinhart GA. Weekly digestibilities of diets supplemented with corn oil, lard or tallow by weanling swine. J Anim Sci 1988;66:1430-7. https://doi.org/10.2527/jas1988.6661430x
  28. Jorgensen JN, Laguna JS, Millan C, Casabuena O, Gracia MI. Effects of a Bacillus-based probiotic and dietary energy content on the performance and nutrient digestibility of wean to finish pigs. Anim Feed Sci Technol 2016;221:54-61. https://doi.org/10.1016/j.anifeedsci.2016.08.008
  29. Xing JJ, van Heugten E, Li DF, et al. Effects of emulsification, fat encapsulation, and pelleting on weanling pig performance and nutrient digestibility. J Anim Sci 2004;82:2601-9. https://doi.org/10.2527/2004.8292601x
  30. Li DF, Thaler RC, Nelssen JL, et al. Effect of fat sources and combinations on starter pig performance, nutrient digestibility and intestinal morphology. J Anim Sci 1990;68:3694-704. https://doi.org/10.2527/1990.68113694x
  31. Bee G, Gebert S, Messikommer R. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig. J Anim Sci 2002;80:1564-74. https://doi.org/10.2527/2002.8061564x
  32. Maiorka A, Dahlke F, Santin E, Kessler A, Penz Jr. A. Effect of energy levels of diets formulated on total or digestible amino acid basis on broiler performance. Rev Bras Cienc Avic 2004;6:87-91. https://doi.org/10.1590/S1516-635X2004000200003
  33. Nunez MC, Bueno JD, Ayudarte MV, et al. Dietary restriction induces biochemical and morphometric changes in the small intestine of nursing piglets. J Nutr 1996;126:933-44. https://doi.org/10.1093/jn/126.4.933
  34. Pluske JR, Williams IH, Aherne FX. Villous height and crypt depth in piglets in response to increases in the intake of cows' milk after weaning. Anim Sci 1996;62:145-58. https://doi.org/10.1017/S1357729800014429
  35. Fasina Y, Moran E, Ashwell C, et al. Effect of dietary gelatin supplementation on the expression of selected enterocyte genes, intestinal development and early chick performance. Int J Poult Sci 2007;6:944-51. https://doi.org/10.3923/ijps.2007.944.951
  36. Ferket PR, van Heugten E, van Kempen TATG, Angel R. Nutritional strategies to reduce environmental emissions from nonruminants. J Anim Sci 2002;80(Issue_2):E168-82. https://doi.org/10.2527/animalsci2002.80E-Suppl_2E168x
  37. Ao X, Yan L, Meng QW, et al. Effects of Saururus chinensis extract supplementation on growth performance, meat quality and slurry noxious gas emission in finishing pigs. Livest Sci 2011;138:187-92. https://doi.org/10.1016/j.livsci.2010.12.029
  38. Jo JK, Ingale SL, Kim JS, et al. Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs. J Anim Sci 2012;90:3041-8. https://doi.org/10.2527/jas.2010-3430
  39. Chen SY, Liu ZX, He YD, Chu C, Wang MQ. Effect of coated lipase supplementation on growth, digestion and intestinal morphology in weaning piglets. J Anim Vet Adv 2014;13:1093-7.
  40. Dierick NA, Decuypere JA. Influence of lipase and/or emulsifier addition on the ileal and faecal nutrient digestibility in growing pigs fed diets containing 4% animal fat. J Sci Food Agric 2004;84:1443-50. https://doi.org/10.1002/jsfa.1794
  41. Cho JH, Zhao P, Kim IH. Effects of emulsifier and multi-enzyme in different energy densitydiet on growth performance, blood profiles, and relative organ weight in broiler chickens. J Agric Sci 2012;4:161-8.
  42. Zhang GG, Yang ZB, Wang Y, Yang WR, Zhou HJ. Effects of dietary supplementation of multi-enzyme on growth performance, nutrient digestibility, small intestinal digestive enzyme activities, and large intestinal selected microbiota in weanling pigs. J Anim Sci 2014;92:2063-9. https://doi.org/10.2527/jas.2013-6672
  43. Garry BP, Fogarty M, Curran TP, O'Connell MJ, O'Doherty JV. The effect of cereal type and enzyme addition on pig performance, intestinal microflora, and ammonia and odour emissions. Animal 2007;1:751-7. https://doi.org/10.1017/S1751731107720190
  44. O'Connell JM, Sweeney T, Callan JJ, O'Doherty JV. The effect of cereal type and exogenous enzyme supplementation in pig diets on nutrient digestibility, intestinal microflora, volatile fatty acid concentration and manure ammonia emissions from finisher pigs. Anim Sci 2005;81:357-64. https://doi.org/10.1079/ASC42040357