Fig. 3. V-shaped necrotic tissues by cause of wound/drop inoculation of (A, E) Type 1, C. fioriniae; (B, F) Type 2, C. nymphaeae; (C, G) Type 3, C. siamense; (D, H) Type 4, C. fructicola on (A, B, C, D) Fuji cultivar and (E, F, G, H) Aori cultivar. Necrotic tissues symptoms were 14 days after inoculation (DAI).
Fig. 4. Maximum parsimony analysis showing phylogenetic relationships among 18 isolates of Colletotrichum from apple and other related Colletotrichum spp. based on combined dataset of internal transcribed spacer (ITS) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene sequences. Numbers beside each branch represent bootstrap values obtained after a bootstrap test with 1,000 replications. Present isolates are shown in boldface. Bar indicates the number of nucleotide substitutions. The tree is rooted with Colletotrichum orbiculare.
Fig. 1. (A) Colony morphology of 4 selected Colletotrichum species on PDA and V8 juice media. (B) Morphological characteristics of conidia and appressoria of Colletotrichum species in Type 1-4. I, Conidia; II-III, Appressoria. Bars = 10 μm.
Fig. 2. (A) Symptoms on apple fruits after wound/drop inoculation with Colletotrichum spp. Symptoms produced by (I, IV) Type 1, C. fioriniae; (II, VII) Type 2, C. nymphaeae; (III, VIII) Type 3, C. siamense; (IV, IX) Type 4, C. fructicola; (V, X) control fruits. Fruits (I-V) on Fuji cultivar and (VI-X) on Aori cultivar. Lesions were 14 days after inoculation (DAI). (B) Comparison of lesion length among four types of Colletotrichum species on Fuji and Aori cultivars.
Table 1. Sources of isolates used in this study and GenBank accession number of Colletotrichum spp. isolated from apple fruits
Table 2. Description of morphological characters of isolated Colletotrichum species based on colony character, conidial shape, and appressoria
Table 3. Pathogenicity and fruits lesion diameter of apple (Fuji and Aori cultivars) fruits caused by four Colletotrichum species 14 days after inoculation with wound/drop method a