Comparative Study on the Effects of Korean and Chinese Crataegus pinnatifida on Scopolamine-induced Memory Impairment in Mice

Scopolamine 유발 기억 손상 마우스 모델에서 국산 산사와 중국산 산사의 항건망 효과 비교

  • 이지혜 (대구한의대학교 약리학연구실) ;
  • 김혜정 (대구한의대학교 약리학연구실) ;
  • 이찬희 (대구한의대학교 약리학연구실) ;
  • 박상혁 (대구한의대학교 약리학연구실) ;
  • 정철종 ((주)옥천당) ;
  • 백경연 ((주)옥천당) ;
  • 신진기 ((주)옥천당) ;
  • 정지욱 (대구한의대학교 약리학연구실)
  • Received : 2018.09.18
  • Accepted : 2018.11.28
  • Published : 2018.12.25


This study was conducted to investigate the cognitive improvement and memory recovery effects of Korean and Chinese Crataegus pinnatifida ethanolic extracts on scopolamine-induced memory impairment in mice. In vivo studies were carried out with mice treated with Korean Crataegus pinnatifida extracts (KCF) and Chinese Crataegus pinnatifida extracts (CCF) in doses of 5 and 50 mg/kg (p.o.) and scopolamine was injected 30 min before the behavioral testing. Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and acetylcholinesterase inhibition by Ellman's modified method. The chlorogenic acid and hyperoside as marker compounds of KCF and CCF was quantified by ultra-performance liquid chromatography analysis (UPLC). Results showed that KCF was more contained high content of chlorogenic acid and hyperoside than CCF. In addition, KCF was more exerted free radical (DPPH and ABTS) scavenging activity and blocked AChE activity than CCF. In vivo studies also showed that KCF administration has a further improved the memory of scopolamine-treated mice than CCF in Y-maze test, passive avoidance test and Morris water maze test. These results revealed that KCF more prevents scopolamine-induced memory impairments through antioxidant and acethylcholinesterase inhibition effect compared CCF.



Grant : 산사 추출물을 이용한 기억력 및 인지기능 개선용 개별인정형 건강기능식품 개발

Supported by : 연구개발특구진흥재단


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