Influence of oxytetracycline on the fate of Nitrogen species in a recirculating aquaculture system

  • Medriano, Carl A.D. (Program in Environmental Technology and Policy, Korea University) ;
  • Yoon, Hyojik (Program in Environmental Technology and Policy, Korea University) ;
  • Chandran, Kartik (Department of Earth and Environmental Engineering, Columbia University) ;
  • Khanal, Samir.K. (Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa) ;
  • Lee, Jaewoo (Program in Environmental Technology and Policy, Korea University) ;
  • Cho, Yunchul (Department of Environmental Engineering, Daejeon University) ;
  • Kim, Sungpyo (Program in Environmental Technology and Policy, Korea University)
  • Received : 2016.09.05
  • Accepted : 2017.11.20
  • Published : 2018.03.25


Common aquaculture practices include the use of certain pharmaceuticals such as antibiotics in avoiding diseases and promoting a healthier growth of the culture. The aim of this study is to monitor and assess the influence of different low oxytetracycline concentrations on the transformation of nitrogen compounds under aeration condition in a lab-scale recirculating aquaculture system (RAS). Over $1mg\;L^{-1}$ dose of oxytetracycline to aquaculture had induced ammonia($NH_4-N$), nitrate($NO_3-N$), soluble COD accumulation in RAS. In addition, nitrous oxide ($N_2O$) emission from RAS was significantly reduced during the oxytetracycline dose periods. After ceasing the dose of oxytetracycline, ammonia oxidation and nitrous oxide re-emission were observed. This observation indicated that low concentrations of oxytetracycline could affect the nitrogen species in RAS. Also, the emission mechanisms of $N_2O$ may not be only dependent on nitrification process but also dependent on denitrification process in our RAS system.


Supported by : Korea University


  1. Anderson, I.C., Poth, M., Homstead, J. and Burdige, D. (1993), "A comparison of NO and N2O production by the autotrophic nitrifier nitrosomonas europaea and the heterotrophic nitrifer alcaligenes faecalis", Appl. Environ. Microbiol., 59, 3525-3533
  2. Bebak-Williams, J., Bullock, G. and Carson, M.C. (2002), "Oxytetracycline residues in a freshwater recirculating system", Aquacult., 205(3-4), 221-230
  3. Benbrook, C.M., (2002), Antibiotic Drug Use in U.S. Aquaculture, Institute for Agriculture and Trade Policy Report 2.
  4. Bock, E., Schmidt, I., Stuven, R. and Zart, D. (1995), "Nitrogen loss caused by denitrifying Nitrosomonas cells using ammonium or hydrogen as electron donors and nitrite as electron acceptor", Arch. Microbiol., 163(1), 16-20.
  5. Carmignani, G.M. and Bennett, J.P. (1977), "Rapid start-up of a biological filter in a closed aquaculture system", Aquacult., 11(1), 85-88.
  6. Chandran, K. and Love, N.G. (2008), "Physiological state, growth mode, and oxidative stress play a role in Cd (II)-mediated inhibition of Nitrosomonas europaea 19718", Appl. Environ. Microbiol., 74(8), 2447-2453.
  7. Collins, M.T., Gratzek, J.B., Shotts, E.B., Dawe, D.L., Campbell, L.M. and Senn, D.R. (1975), "Nitrification in an aquatic recirculating system", J. Fish. Board Can., 32(11), 2025-2031.
  8. Datta, A., Nayak, D.R., Sinhababu, D.P. and Adhya, T.K. (2009), "Methane and nitrous oxide emissions from an integrated rainfed rice-fish farming system of Eastern India", Agric. Ecosyst. Environ., 129(1-3), 228-237.
  9. Ebeling, J.M. and Timmons, M.B. (2012), "Recirculating aquaculture systems", Aquacult. Prod. Syst., 245-277.
  10. Eichner, M.J. (1989), "Nitrous oxide emissions from fertilized soils: Summary of available data", J. Ennviron. Qual., 19(2), 272-280
  11. Elia, A.C., Ciccotelli, V., Pacini, N., Dorr, A.J.M., Gili, M., Natali, M., Gasco, L., Prearo, M. and Abete, M.C. (2014), "Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant system in liver and kidney", Fish Physiol. Biochem., 40(4), 1055-1068.
  12. FAO (2012), The State of the World Fisheries and Aquaculture, Food and Agriculture Organization of the United Nations, Rome, Italy.
  13. Hargreaves, J.A. (1998), "Nitrogen biogeochemistry of aquaculture ponds", Aquacult., 166(3-4), 181-212.
  14. Hyman, M.R. and Arp, D.J. (1992), "14C2H2-and14CO2-labeling studies of the de novo synthesis of polypeptides by Nitrosomonas europaea during recovery from acetylene and light inactivation of ammonia monooxygenase", J. Biol. Chem., 267, 1534-1545.
  15. Klaver, A.L. and Matthews, R.A. (1994), "Effects of oxytetracycline on nitrification in a model aquatic system", Aquacult., 123(3-4), 237-247.
  16. Kox, M.A. and Jetten, M.S. (2015), The Nitrogen Cycle, Principles of Plant-Microbe Interactions, Springer, 205-214.
  17. Lalumera, G.M., Calamari, D., Galli, P., Castiglioni, S., Crosa, G. and Fanelli, R. (2004), "Preliminary investigation on the environmental occurrence and effects of antibiotics used in aquaculture in Italy", Chemosphere, 54(5), 661-668.
  18. Lawson, T.B. (1995), Fundamentals of Aquacultural Engineering, Springer Science & Business Media, Boston, Massachusetts, U.S.A., 12-39.
  19. Lee, H.J., Lee, T.S., Son, K.T., Kim, P.H., Jo, M.R., Park, M.J. and Yi, Y.H. (2005), "Analysis of tetracyclines using highperformance liquid chromatography for fishery products", J. Kor. Fish. Soc., 38, 372-378.
  20. Levy-Booth, D.J., Prescott, C.E. and Grayston, S.J. (2014), "Microbial functional genes involved in nitrogen fixation, nitrification and denitrification in forest ecosystems", Soil Biol. Biochem., 75, 11-25.
  21. Liu, W., Pan, N., Chen, W., Jiao, W. and Wang, M. (2012), "Effect of veterinary oxytetracycline on functional diversity of soil microbial community", Agricult., 58, 295-301
  22. Losordo, T. and Masser, M. (1998), Recirculating Aquaculture Tank Production Systems: An Overview of Critical Considerations, Southern Regional Aquaculture Center, SRAC Publication, 451.
  23. Metcalf Eddy, Burton, F.L., Stensel, H.D. and Tchobanoglous, G. (1979), Wastewater Engineering: Treatment and Reuse, McGraw-Hill Book Company, New York, U. S.A.
  24. Moyer, C.L., Dobbs, F.C. and Karl, D.M. (1994), "Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system Loihi Seamount, Hawaii", Appl. Environ. Microbiol., 60(3), 871-879.
  25. Muyzer, G., De Waal, E.C. and Uitterlinden, A.G. (1993), "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA", Appl. Environ. Microbiol., 59(3), 695-700.
  26. Nakano, T., Hayashi, S. and Nagamine, N. (2015), "Effect of excessive doses of oxytetracycline on stress-related biomarker expression in coho salmon", Environ. Sci. Pollut. Res., 1-8.
  27. Nazar, A.A., Jayakumar, R. and Tamilmani, G. (2013), Recirculating Aquaculture Systems.
  28. Okabe, S., Oshiki, M., Takahashi, Y. and Satoh, H. (2011), "N2O emmision from a partial nitrification-anammox process and identification of a key biological process of N2O emmision from anamox granules", Water Res., 45(19), 6461-6470
  29. Pilegaard, K. (2013), "Processes regulating nitric oxide emissions from soils", Phil. Trans. R. Soc. B, 368(1621), 20130126.
  30. Randall, D.J. and Tsui, T.K.N. (2002), "Ammonia toxicity in fish", Mar. Pollut. Bull., 45(1-12), 17-23.
  31. Rigos, G. and Smith, P. (2015), "A critical approach on pharmacokinetics, pharmacodynamics, dose optimisation and withdrawal times of oxytetracycline in aquaculture", Rev. Aquacult., 7(2), 77-106.
  32. Rodrigues, R.V., Schwarz, M.H. and Delbos, B.C. (2007), "Acute toxicity and sublethal effects of ammonia and nitrite for juvenile cobia Rachycentron canadum", Aquacult., 271(1-4), 553-557.
  33. Rotthauwe, J.H., Witzel, K.P. and Liesack, W. (1997), "The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammoniaoxidizing populations", Appl. Environ. Microbiol., 63(12), 4704-4712.
  34. Suga, N., Ogo, M. and Suzuki, S. (2013), "Risk assessment of oxytetracycline in water phase to major sediment bacterial community: A water-sediment microcosm study", Environ. Toxicol. Pharmaco., 36(1), 142-148.
  35. Svobodova, Z., Machova, J., Poleszczuk, G., Huda, J., Hamackova, J. and Kroupova, H. (2005), "Nitrite poisoning of fish in aquaculture facilities with water-recirculating systems", Acta Vet. Brno., 74(1), 129-137.
  36. USEPA (2009), Draft 2009 Update: Aquatic Life Ambient Water Quality Criteria for Ammonia-Freshwater, EPA-82-D-09-0012009.
  37. Ward, B.B., Arp, D.J. and Klotz, M.G. (2011), Nitrification, American Society for Microbiology Press, Washington, D.C., U.S.A.
  38. Williams, J. and Crutzen, P. (2010), "Nitrous oxide from aquaculture", Nat. Geosci., 3(3), 143-143.