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Enhancing Astaxanthin Accumulation in Haematococcus pluvialis by Coupled Light Intensity and Nitrogen Starvation in Column Photobioreactors

  • Zhang, Wen-wen (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University) ;
  • Zhou, Xue-fei (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University) ;
  • Zhang, Ya-lei (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University) ;
  • Cheng, Peng-fei (Poyang Lake Eco-economy Research Center, Jiujiang University) ;
  • Ma, Rui (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University) ;
  • Cheng, Wen-long (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University) ;
  • Chu, Hua-qiang (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University)
  • Received : 2018.07.09
  • Accepted : 2018.10.24
  • Published : 2018.12.28

Abstract

Natural astaxanthin mainly derives from a microalgae producer, Haematococcus pluvialis. The induction of nitrogen starvation and high light intensity is particularly significant for boosting astaxanthin production. However, the different responses to light intensity and nitrogen starvation needed to be analyzed for biomass growth and astaxanthin accumulation. The results showed that the highest level of astaxanthin production was achieved in nitrogen starvation, and was 1.64 times higher than the control group at 11 days. With regard to the optimization of light intensity utilization, it was at $200{\mu}mo/m^2/s$ under nitrogen starvation that the highest astaxanthin productivity per light intensity was achieved. In addition, both high light intensity and a nitrogen source had significant effects on multiple indicators. For example, high light intensity had a greater significant effect than a nitrogen source on biomass dry weight, astaxanthin yield and astaxanthin productivity; in contrast, nitrogen starvation was more beneficial for enhancing astaxanthin content per dry weight biomass. The data indicate that high light intensity synergizes with nitrogen starvation to stimulate the biosynthesis of astaxanthin.

Acknowledgement

Supported by : National Natural Science Foundation of China

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