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Scanning electron microscopy of filiform papillae development in Korean native goats (Capra hircus)

  • Lee, Si-Joon (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Cho, Gyu-Hyen (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Mun-Ki (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Chong-Sup (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University) ;
  • Won, Chung-Kil (Institute of Animal Medicine & Department of Veterinary Medicine, Gyeongsang National University)
  • Received : 2018.09.06
  • Accepted : 2018.11.09
  • Published : 2018.12.31

Abstract

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

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Fig. 1. Scanning electron micrographs showing prenatal filiform papillae (FP) of Korean native goats. (A) Undeveloped primordia of FP appeared in 60-day-old fetus (embryonic day [E] 60). (B) High magnification view on the surface of FP in E60. (C) Developing primordia of FP are seen in 90-day-old fetus. (D) FP shaped like a double flower bud in 120-day-old fetus (E120). (E) High magnification view on the surface of FP in E120. (F) FP have empty inside and irregularly arranged saw blade-like processes in neonates. FUP, fungiform papillae; MP, microplicae. Scale bars = 100 μm (A, B, D and F), 1 μm (B and E).

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Fig. 2. Scanning electron micrographs showing postnatal FP from juveniles of Korean native goats. (A) FP have big and long primary papillae with narrow and low secondary papillae in 30-day-old goat. (B) FP of 60-day-old goat and (C) FP of 90-dayold goat showed well-developed secondary papillae. (D) FP of 120-day-old goat and (E) FP of 150-day-old goat had many small peaked papillae processes. (F) FP of 180-day-old goat had completed morphogenesis. Scale bars = 100 μm.

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Fig. 3. High magnification view on the surface of postnatal developing FP in Korean native goats. (A) Many MP are developed on the surface of FP in 30-day-old goat. (B) MP are long and straight shaped like thread while microridge (MR) is observed in 60-day-old goat. (C) MP and MR are well-developed in 90-day-old goat. (D) The surface epithelium of FP has straight MP in 180-day-old goat. Scale bars = 1 μm.

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Fig. 4. Scanning electron microscopy measurements of the length of FP. Each height and oblique length were measured from postnatal day 0 (P0) to 180-day-old Korean native goats (P180). There were no statistically significant differences in size of FP during the next 30 days (p > 0.05).

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