Serologic monitoring of animal welfare-oriented laying-hen farms in South Korea

  • So, Hyunhee (College of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Seolryung (College of Veterinary Medicine, Chungbuk National University) ;
  • Mo, Jongsuk (College of Veterinary Medicine, Chungbuk National University) ;
  • Min, Kyungchul (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Jongnyeo (College of Veterinary Medicine, Chungbuk National University) ;
  • Mo, In-Pil (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2018.09.21
  • Accepted : 2018.12.19
  • Published : 2018.12.31


As animal welfare issue becomes important, the European Union bans conventional cages for laying hens from 2012. So the alternative housing systems like floor pens, aviaries or free range systems have been suggested. From 2011 to 2014, we monitored 20 welfare-oriented laying hen farms in South Korea to figure out serological status of major viral diseases. During this period, total 3,219 blood samples were collected from the randomly selected chickens to test and evaluate the hemagglutination inhibition titers for low pathogenic avian influenza, Newcastle disease and egg drop syndrome '76. A total of 2,926 blood samples were tested through enzyme linked immunosorbent assay (ELISA) to assess the serological status of infectious bronchitis (IB). The distribution of ELISA titers for IB was various from almost 0 to 20,000 through the all weeks of age. Also, the antibody coefficient of variation for most of the diseases in this study was higher than those of typical cage layers. As this study was the first surveillance for major avian viral diseases of the animal welfare-oriented farms in South Korea, the results obtained from this study will help to determine what information and resources are needed to maintain better biosecurity and to improve the health and welfare of laying hen flocks.

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Fig. 1. Distribution of ELISA titers of the specific antibodies against IB virus in individual birds of the layer flocks in the animal wel-fare-oriented farms from 2011 to 2014.

Table 2. Number of blood samples tested for the main viral avian diseases from year 2011 to 2014

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Table 1. The vaccination program for the animal-welfare layer farms in this study

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Table 3. Comparison of hemagglutination inhibition (HI) titer against ND virus monitored for the layer flocks in the animal welfare-oriented farms from year 2011 to 2014

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Table 4. Reference table from a study on serological results against major viral diseases for caged layer flocks [15]

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Table 5. Comparison of HI titer against H9N2 avian influenza virus monitored for the layer flocks in the animal welfare-oriented farms from year 2011 to 2014

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Table 6. Comparison of HI antibody titer against EDS’76 virus monitored for the layer flocks in the animal welfare-oriented farms from year 2011 to 2014

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Table 7. Comparison of enzyme-linked immunosorbent assay (ELISA) antibody titer against IB virus monitored for the layer flocks in the animal welfare-oriented farms from year 2011 to 2014

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Table 8. Comparison of maternal antibodies level of AI (H9N2) and ND monitored for day-old flocks in the animal welfare-oriented farms collected from year 2011 to 2014

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Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)


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