Antioxidant effect and inhibitory activities of ethyl acetate fraction from Gardenia jasminoides extract on nitric oxide production and pancreatic cancer cell proliferation

치자 에틸아세테이트 분획의 산화방지, 산화질소 제거 및 암세포증식 억제 활성

  • Park, Min Kyung (Department of Natural Medicine Resources, Semyung University) ;
  • Yoon, Hye Ji (Department of Natural Medicine Resources, Semyung University) ;
  • Lee, Hwa Jin (Department of Natural Medicine Resources, Semyung University)
  • 박민경 (세명대학교 자연약재과학과) ;
  • 윤혜지 (세명대학교 자연약재과학과) ;
  • 이화진 (세명대학교 자연약재과학과)
  • Received : 2017.11.16
  • Accepted : 2018.01.11
  • Published : 2018.04.30


To evaluate the radical scavenging activity of phenolic-rich fractions of Gardenia jasminoides, we first measured the levels of total polyphenols in hexane, ethyl acetate, and butanol fractions from the extract of G. jasminoides. The ethyl acetate fraction of G. jasminoides extract (GJ-EA) showed high level of phenolics, potent reducing power, and 2,2-diphenyl-1-picrylhydrazyl/2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid radical scavenging effect. In addition, GJ-EA inhibited the overproduction of nitric oxide in lipopolysaccharide-activated BV-2 microglia. Furthermore, we found that GJ-EA suppressed $H_2O_2$-induced PANC-1 pancreatic cancer cell proliferation in a concentration-dependent manner and also reduced their migratory ability. These results suggest that GJ-EA may be a good source for functional foods with antioxidant and chemo-preventive activities.


Supported by : 세명대학교


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