DOI QR코드

DOI QR Code

Enumeration of Weissella cibaria phage with cytometry, epifluorescence microscopy, and plaque assay

유세포분석기, 형광현미경, 용균반검사 분석을 이용한 Weissella cibaria 박테리오파지 정량분석 및 상관관계분석

  • Received : 2018.02.02
  • Accepted : 2018.02.21
  • Published : 2018.04.30

Abstract

Quantitative analysis for non-host infection bacteriophage was conducted for their enumeration. Flow cytometry and epifluorescence microscopy (EPM) were selected as counting methods. Correlation analysis was performed based on the plaque assay method on the existing host infection and consisted of Pearson correlation statistical analysis, regression analysis, and difference analysis. Analyses of 12 samples with flow cytometry and plaque assay methods showed that there was a correlation of 96.7% with Pearson correlation value r=0.967, $R^2$ 0.9352, and difference value of 1.063. Analyses of 12 samples with EPM and plaque assay methods showed that there was a correlation of 99.0% with Pearson correlation value r=0.990, $R^2$ 0.9811, and difference value of 1.605. Therefore, flow cytometry and epifluorescence microscopy would be effective for enumeration of Weissella cibaria bacteriophage with plaque assay.

Keywords

bacteriophage;flow cytometry;epifluorescence microscopy;W. cibaria bacteriophage;quantitative analysis

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Acknowledgement

Supported by : 한국연구재단