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Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells

LPS로 유발한 RAW264.7 세포 염증반응에 대한 가감통순산(加減通順散)의 억제 효과

  • Received : 2018.03.19
  • Accepted : 2018.04.02
  • Published : 2018.04.30

Abstract

Objectives This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2. Methods Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA). Results The GT does not impair the cell viability in tested concentration $500{\mu}g/ml$ or below. GT significantly reduced the NO production in a dose-dependent manner. GT $500{\mu}g/ml$ also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT $500{\mu}g/ml$ reduced the PGE2 secretion in LPS induced RAW264.7 cells. Conclusions These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT's anti-inflammatory effect objectively.

Keywords

Gagamtongsoon-San(GT);Anti-inflammatory;RAW264.7;LPS

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