Effect of Crocus sativus L. stigma (saffron) against subacute effect of diazinon: histopathological, hematological, biochemical and genotoxicity evaluations in rats

  • Hariri, Alireza Timcheh (Medical Toxicology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences) ;
  • Moallem, Seyed Adel (Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences) ;
  • Mahmoudi, Mahmoud (Immunology Research Center, Mashhad University of Medical Sciences) ;
  • Memar, Bahram (Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences) ;
  • Razavi, Bibi Marjan (Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences) ;
  • Hosseinzadeh, Hossein (Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences)
  • Received : 2017.05.10
  • Accepted : 2018.03.30
  • Published : 2018.06.30


Objective: In this study, the effects of saffron stigma against subacute diazinon (DZN) toxicity on enzymes levels, biochemical, hematological, histopathological and genotoxicity indices were studied in rats. Methods: Vitamin E (200 IU/kg) and the aqueous extract of saffron (50, 100 and 200 mg/kg) were injected intraperitoneally three times per week alone or with DZN (20 mg/kg/day, orally) for 4 weeks. The hematological and biochemical parameters were evaluated at the end of 4 weeks. Results: Reticulocytes counts, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine phosphokinase, CPK-MB, gama glutamyl transferase (GGT), uric acid and micronucleus indices were increased significantly but total protein and RBC cholinesterase activity were decreased in the DZN-treated group. Saffron prevented the effect of DZN on GGT (50 mg/kg), LDH, CPK and CPK-MB (100 and 200 mg/kg) levels. An increased uric acid and reduced protein levels by DZN were prevented by vitamin E and some doses of saffron. A significant reduction was observed in platelets, RBC, hemoglobin and hematocrit indices in the DZN group. Saffron and vitamin E prevented this reduction. Vitamin E and saffron did not reduce the effect of DZN on RBC cholinesterase activity. The extract and vitamin E could not prevent DZN genotoxicity in the micronucleus assay. Other biochemical parameters and pathological evaluation did not show any abnormality in tissues of all groups. Conclusion: This study shows that vitamin E and saffron reduce DZN induced hematological and biochemical toxicity. However, they do not prevent the genotoxicity induced by DZN.


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