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Mycelial growth and wood decaying enzymatic activity analysis by various addition rates of oak powder in the liquid spawn of Lentinula edodes

참나무분 첨가에 따른 표고 액체종균의 균체생산 및 효소 활성

  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Kang, Young-Ju (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Baek, Il-Sun (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Jeoung, Yun-Kyeoung (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Yong-Seon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Cho, Hae-Seok (Chungwoon Mushroom Farm) ;
  • Lee, Young-Soon (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services)
  • 김정한 (경기도농업기술원 버섯연구소) ;
  • 강영주 (경기도농업기술원 버섯연구소) ;
  • 백일선 (경기도농업기술원 버섯연구소) ;
  • 정윤경 (경기도농업기술원 버섯연구소) ;
  • 이용선 (경기도농업기술원 버섯연구소) ;
  • 조해석 (청운표고(주)) ;
  • 이영순 (경기도농업기술원 버섯연구소)
  • Received : 2018.03.19
  • Accepted : 2018.06.25
  • Published : 2018.06.30

Abstract

This study was carried out to establish a suitable method for liquid spawn production from Lentinula edodes. The optimum production of liquid spawn (OLS) was achieved using soybean meal medium (SMM) with 0.3% of 850 um oak powder and 10-day incubation period and 0.6 vvm aeration volume. OLS showed activities of laccase on ABTS agar plate and carboxymethyl cellulase (CM-cellulase) on CMC agar plate. In case of liquid spawn, fruiting-body development period was delayed approximately 1 day compared to that of sawdust spawn, however, the yield of 153 g per 1.2 kg polypropylene bag was similar to that of sawdust spawn.

Acknowledgement

Supported by : 농림수산식품기술기획평가원

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