Mycelial growth and wood decaying enzymatic activity analysis by various addition rates of oak powder in the liquid spawn of Lentinula edodes

참나무분 첨가에 따른 표고 액체종균의 균체생산 및 효소 활성

  • Received : 2018.03.19
  • Accepted : 2018.06.25
  • Published : 2018.06.30


This study was carried out to establish a suitable method for liquid spawn production from Lentinula edodes. The optimum production of liquid spawn (OLS) was achieved using soybean meal medium (SMM) with 0.3% of 850 um oak powder and 10-day incubation period and 0.6 vvm aeration volume. OLS showed activities of laccase on ABTS agar plate and carboxymethyl cellulase (CM-cellulase) on CMC agar plate. In case of liquid spawn, fruiting-body development period was delayed approximately 1 day compared to that of sawdust spawn, however, the yield of 153 g per 1.2 kg polypropylene bag was similar to that of sawdust spawn.


Lentinula edodes;Liquid spawn;Oak powder;Wood decaying enzyme;Mycelia amount


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Supported by : 농림수산식품기술기획평가원