DOI QR코드

DOI QR Code

Mycelial growth and wood decaying enzymatic activity analysis by various addition rates of oak powder in the liquid spawn of Lentinula edodes

참나무분 첨가에 따른 표고 액체종균의 균체생산 및 효소 활성

  • Received : 2018.03.19
  • Accepted : 2018.06.25
  • Published : 2018.06.30

Abstract

This study was carried out to establish a suitable method for liquid spawn production from Lentinula edodes. The optimum production of liquid spawn (OLS) was achieved using soybean meal medium (SMM) with 0.3% of 850 um oak powder and 10-day incubation period and 0.6 vvm aeration volume. OLS showed activities of laccase on ABTS agar plate and carboxymethyl cellulase (CM-cellulase) on CMC agar plate. In case of liquid spawn, fruiting-body development period was delayed approximately 1 day compared to that of sawdust spawn, however, the yield of 153 g per 1.2 kg polypropylene bag was similar to that of sawdust spawn.

Keywords

Lentinula edodes;Liquid spawn;Oak powder;Wood decaying enzyme;Mycelia amount

References

  1. Hong SJ, Lee WH, Shin BS, Sung JM. 2003. Production of Pleurotus ostreatus and Flammmmulina velutipes using liquid spawn inoculation system. Kor J Mycol. 31:22-27(in Korean). https://doi.org/10.4489/KJM.2003.31.1.022
  2. Jang MJ, Lee YH, Ju YC, Koo HM. 2010. Cultural characteristics by sawdust and liquid spawn for the cultivation of Neolentinus lepideus. Kor J Mycol. 38:125-129(in Korean). https://doi.org/10.4489/KJM.2010.38.2.125
  3. Joen SM, Ka KH. 2014. Mycelial growth and extracelluar enzyme activities of wood-decaying mushroom strains on solid media. Kor J Mycol. 42:40-49(in Korean). https://doi.org/10.4489/KJM.2014.42.1.40
  4. Kawai G, Kobayashi H, Fukushima Y, Ohsaki K. 1996. Effect of liquid mycelial culture used as a spawn on sawdust cultivation of shiitake (Lentinula edodes). Mycoscience. 37:201-207. https://doi.org/10.1007/BF02461345
  5. Lee BH, Bak WC, Yoon KH. 2006. Comparison of shiitake productivity in sawdust media according to the use of sawdust, plug-shaped and liquid spawn. Kor J Mycol. 34:79-83(in Korean). https://doi.org/10.4489/KJM.2006.34.2.079
  6. Shim KK, Yoo YJ, Koo CD, Kim MK. 2014. Changes of nutrients in media and mycelia on liquid spawn culture of Lentinula edodes. Kor J Mycol. 42:144-149(in Korean). https://doi.org/10.4489/KJM.2014.42.2.144

Acknowledgement

Supported by : 농림수산식품기술기획평가원