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The Anti-obesity Effect of Seungyangjeseup-tang for High Fat Diet Induced Obese Mice

고지방식이 유도 비만 생쥐에 대한 승양제습탕의 항비만 효과

  • Kim, Jung-Min (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Hanny University) ;
  • Choi, Soo-Min (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Hanny University) ;
  • Woo, Chang-Hoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Hanny University) ;
  • Ahn, Hee-Duk (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Hanny University)
  • 김정민 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 최수민 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2018.06.08
  • Accepted : 2018.07.05
  • Published : 2018.07.31

Abstract

Objectives This study was designed to evaluate the efficacy of Seungyangjeseup-tang on obesity by using 3T3-L1 cells and high fat diet mice. Methods In vitro, Seungyangjeseup-tang extract (SYJST) (10, 50, 100, 200, 400, $800{\mu}g/mL$) ware added in 3T3-L1 cells. SYJST cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assasy. Adipocyte differentiation was measured by Oil Red-O staining. In vivo, the experimental animals were divided into five groups: normal diet-fed normal group (N), high-fat diet (HFD)-fed control group (Con), HFD+SYJST 100 mg/kg group (SY100), HFD+SYJST 200 mg/kg group (SY200), and HFD+olistat 60 mg/kg as a positive drug control group (Orli). Markers of obesity, such as body weight, liver weight, food intake, serum total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), liver tissue TC, TG and fecal TC, TG were measured. Results In vitro, cytotoxicity was not significant compared with the control group. 3T3-L1 cell's differentiation was significantly decreased in Oil Red-O staining. In vivo, compared with controls, mice treated with SYJST demonstrate lower body and liver weight, and reduced food intake. In addition, SYJST increased TC, TG in the serum but not significance. And SYJST showed decreasing tendency TC, TG in the liver tissue. Furthermore, SYJST increased TC, TG in the facal but not significance. Conclusions Based on the results above, Seungyangjeseup-tang may reduce adipocyte differentiation, body fat, food intake, liver weight in obesity. This suggests that Seungyangjeseup-tang may be clinically useful in obesity treatment.

Keywords

Seungyangjeseup-tang;Obesity;3T3-L1 cell

References

  1. Korean Society for the Study of Obesity. Obesity treatment guidelines. Seoul:Korean Society for the Study of Obesity. 2012:14.
  2. Powell AG, Apovian CM, Aronne LJ. New drug targets for the treatment of obesity. Clin Pharmacol Ther. 2011;90(1):40-51. https://doi.org/10.1038/clpt.2011.82
  3. Padwal RS, Majumdar SR. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet. 2007;369(9555):71-7. https://doi.org/10.1016/S0140-6736(07)60033-6
  4. Yin J, Zhang H, Ye J. Traditional chinese medicine in treatment of metabolic syndrome. Endocr Metab Immune Disord Drug Targets. 2008;8(2):99-111. https://doi.org/10.2174/187153008784534330
  5. Kim DH, Lee JH. Hwangjenaegyeong somun. 1st ed. Seoul:Euiseongdang. 2002:652, 974.
  6. The Society of Korean Medicine Rehabilitation. Korean Rehabilitation Medicine. 4th ed. Paju:Koonja Publishing. 2015:298-317.
  7. Jeong JW. Trend research of the human body-oriented obesity studies on oriental medicine. [dissertation]. Busan (KR): Dong-Eui University; 2016.
  8. Heo J. Donguibogam. 4th ed. Gyeongnam: Donguibogam Publisher. 2010:387.
  9. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957;226(1):497-509.
  10. Korea Centers for Disease Control and Prevention. 2009 Korea national health and nutrition and examination survey. Cheongju:Ministry of Health and Welfare. 2010:1-14.
  11. Suh YS. Condiderable causes of obesity. Journal of the Korean Society of Biological Therapies in Psychiatry. 2002;8(2):218-24.
  12. Song DK, Sung YA. Obesity: introduction. Korean J Med. 2013;84(5):619-23. https://doi.org/10.3904/kjm.2013.84.5.619
  13. Kim KS, Park SW. Drug therapy for obesity. Korean J Obes. 2012;21(4):197-202. https://doi.org/10.7570/kjo.2012.21.4.197
  14. Woo HC, Shin BK, Cho IJ, Koo HN, Kim MH, Han JH. Anti-obesity effect of carbon dioxide supercritical fluid extracts of panax ginseng C. A. Meyer. J Korean Soc Appl Biol Chem. 2011;54(5):738-43. https://doi.org/10.1007/BF03253153
  15. Shin IS, Choi HM, Ku SK, Kim MR. The effect of natural mixture supplementation on histopathological and histomorphometrical aspects in high fat diet-induced obese mice. Kor J Herbaology. 2012;27(4):53-8.
  16. Lee JS, Lee SH. The reductive effects of oriental medicine on the body fat and abdominal obesity. J Korean Med Obes Res. 2001;1(1):35-49.
  17. Park BJ. Herbal diet of obese with osteoarthristis. J Korean Med Obes Res. 2004;4(1):201-11.
  18. A herbal medicine compilation committee of a college of Korean medicine. Herbal medicine. 1st ed. Seoul: Yeonglimsa. 2007:159, 163, 186, 188, 331, 347-8, 389, 412-3, 583.
  19. Youh EJ, Seo BK, Nam SS, Kang SK. Anti-obesity effect of rhizoma atractylodis herbal acupuncture in high fat diet-induced obese ICR Mouse. The Journal of Korean Acupuncture and Moxibustion Society. 2010;27(6):31-42.
  20. Youn DH, Kang JD, Joo JS, Chae WS, Na CS. Effects of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) in obese rats induced by high fat diet. Journal of Korean Institute of Herbal-Acupuncture. 2004;7(1): 87-100.
  21. Joo JS, Youn DH, Na CS, Cho MR, Chae WS. Effects of herbal acupuncture (Atratylodes japonica Koizumi and Coix lachrymajobi var. mayeun Stapf) at Pungnyung(ST 40) and Umnungchon(SP9) in obese rats induced by high fat diet. The Journal of Korean Acupuncture and Moxibustion Society. 2004;21(2):131-54.
  22. Paik SH, Han SR, Kwon OJ, Ahn YM, Ahn SY, Lee BC. The effects in metabolism and adipose tissue inflammation induced by the Massa Medicata Fermentata on obese type 2 diagetes mouse model. J Korean Oriental Med. 2012;33(3):33-45.
  23. Jeong HS. Efficacy of Alismatis Orientale Rhizoma on obesity induced by high fat diet. Kor J Herbology. 2013;28(3):95-106. https://doi.org/10.6116/kjh.2013.28.3.95
  24. Jo HK, Han MH, Hong SH, Choi YH, Park C. Ethanol extracts of citrus peel inhibits adipogenesis through AMPK signaling pathway in 3T3-L1 preadipocytes. J Life Sci. 2015;25(3);285-92. https://doi.org/10.5352/JLS.2015.25.3.285
  25. Park CH, Jung HK, Jeong YS, Hong JH, Lee GD, Park CD. Effects of citrus peel ethanol extract on the serum lipid and body fat of high-fat-diet-fed rats. Korean J Food Preserv. 2011;18(4):567-74. https://doi.org/10.11002/kjfp.2011.18.4.567
  26. Lee JE, Cho MR, Ryu CR. Original Article: The effects of herbal acupuncture (Fructus Hordei Germiniatus) at acupoing Jung-wan(CV12) on the obese rats induced by high fat diet. The Journal of Korean Acupuncture and Moxibustion Society. 2008;25(2):211-26.
  27. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Genes Dev. 2000;14(11):1293-307.
  28. Gregorie FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol Rev. 1998;78(3):783-809. https://doi.org/10.1152/physrev.1998.78.3.783
  29. DiGirolamo M, Fine JB, Tagra K, Rossmanith R. Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum. Am J Physiol. 1998;274(5 Pt 2):R1460-7.
  30. Gerhard M, William HS. Principles of medical biochemistry. 2nd ed. Seoul:Jeongmungag. 2005:407-67.
  31. Arther CC, John EH. Textbook of medical physiology. 1st ed. Seoul:Jeongdam. 2002;915-6.
  32. Min HG. Endocrinology. 1st ed. Seoul:Korea Medical Book Publishing Company. 1999:553-4, 1149, 1203, 1206-8, 1212.
  33. Lee SY, Jeong YS, Gwon OH, Song GS. Clinical pathology test method. 7th ed. Seoul:Yonsei University Publishing. 2000:212-23, 224-8, 242-5, 262-6, 303-6, 307-8, 313-5, 371-2.
  34. The Korean Society of Pathologists. Clinical Pathology. 3rd ed. Seoul:Korea Medical Book Publishing Company. 2001:63-4.
  35. Han LK, Xu BJ, Kimura Y, Zheng Yn, Okuda H. Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. J Nutr. 2000;130(11):2760-4. https://doi.org/10.1093/jn/130.11.2760
  36. Lim DY. Lippincott's illustrated review: pharmacology. 4th ed. Seoul:Shinilbooks. 2009:345-6.
  37. Crenier L, Sternon J. Orlistat(Xenical). Rev Med Brux. 1999;20(3):159-63.
  38. Wong NN, Cheng-Lai A. Orlistat. Heart Dis. 2000;2(2):174-81.